MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 240)

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-81

Accession Score Description
1 NDK_PSEFS 74 Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1
Score Mass Matches Sequences emPAI
81.1 NDK_PSEFS 74 14928 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain SBW25) GN=ndk PE=3 SV=1
8 samesets of NDK_PSEFS
NDK_PSEPG 74 14944 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas putida (strain GB-1) GN=ndk PE=3 SV=1
NDK_PSEPW 74 15002 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas putida (strain W619) GN=ndk PE=3 SV=1
NDK_PSESM 74 14942 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas syringae pv. tomato GN=ndk PE=3 SV=1
NDK_PSEU2 74 14942 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ndk PE=3 SV=1
NDK_PSEF5 74 15073 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=ndk PE=3 SV=1
NDK_PSE14 74 14942 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=ndk PE=3 SV=1
NDK_PSEPK 74 15035 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas putida (strain KT2440) GN=ndk PE=3 SV=1
NDK_PSEP1 74 15035 5 (2) 2 (2) 0.34
Nucleoside diphosphate kinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ndk PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
572   788.4299 787.4226 787.4188 4.87 1 58 0.0022 +1Score > 44 indicates identity U K.EAAAGTIR.A
573   394.7186 787.4226 787.4188 4.90 1 36 0.15 +1Score > 44 indicates identity
Score > 40 indicates homology		 U K.EAAAGTIR.A
1693 +1 430.5888 1288.7446 1288.7391 4.27 0 29 0.23 +1Score > 35 indicates identity U R.TFSIIKPDAVAK.N
1694   645.3802 1288.7458 1288.7391 5.26 0 55 0.00046 +1Score > 35 indicates identity
Score > 34 indicates homology		 U R.TFSIIKPDAVAK.N

2 subsets and intersections (40 subset proteins in total)

Score Mass Subset of
NDK_ARCB4 58 14794 81.1
Nucleoside diphosphate kinase OS=Arcobacter butzleri (strain RM4018) GN=ndk PE=3 SV=1
12 samesets of NDK_ARCB4
NDK_CAMFF 58 14952
Nucleoside diphosphate kinase OS=Campylobacter fetus subsp. fetus (strain 82-40) GN=ndk PE=3 SV=1
NDK_CAMLR 58 15075
Nucleoside diphosphate kinase OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=ndk PE=3 SV=1
NDK_CHRSD 58 15208
Nucleoside diphosphate kinase OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=ndk PE=3 SV=1
NDK_DESAA 58 15154
Nucleoside diphosphate kinase OS=Desulfatibacillum alkenivorans (strain AK-01) GN=ndk PE=3 SV=1
NDK_HALHL 58 15607
Nucleoside diphosphate kinase OS=Halorhodospira halophila (strain DSM 244 / SL1) GN=ndk PE=3 SV=1
NDK_LEPBJ 58 15231
Nucleoside diphosphate kinase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) GN=ndk PE=3 SV=1
NDK_LEPBL 58 15231
Nucleoside diphosphate kinase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) GN=ndk PE=3 SV=1
NDK_MARMS 58 14719
Nucleoside diphosphate kinase OS=Marinomonas sp. (strain MWYL1) GN=ndk PE=3 SV=1
NDK_VIBPA 58 15912
Nucleoside diphosphate kinase OS=Vibrio parahaemolyticus GN=ndk PE=3 SV=1
NDK_CAMC5 58 15038
Nucleoside diphosphate kinase OS=Campylobacter curvus (strain 525.92) GN=ndk PE=3 SV=1
NDK_LEPIC 58 15302
Nucleoside diphosphate kinase OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=ndk PE=3 SV=1
NDK_LEPIN 58 15302
Nucleoside diphosphate kinase OS=Leptospira interrogans GN=ndk PE=3 SV=1
NDK_ALTMD 55 15638 81.1
Nucleoside diphosphate kinase OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=ndk PE=3 SV=1
+26 samesets of NDK_ALTMD

+82

Accession Score Description
1 ODBB_PSEPU 72 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

+83

Accession Score Description
1 RRF_ACTSZ 72 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+84

Accession Score Description
1 GLPD_PSETO 72 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

+85

Accession Score Description
1 ATPE_PSEPG 70 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+86

Accession Score Description
1 RL6_PSEP1 69 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+87

Accession Score Description
1 RL20_PSEE4 68 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1

+88

Accession Score Description
1 RL15_PSEE4 67 50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1

+89

Accession Score Description
1 RL10_PSEPG 66 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+90

Accession Score Description
1 GRPE_PSEPK 65 Protein grpE OS=Pseudomonas putida (strain KT2440) GN=grpE PE=3 SV=1
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