MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 240)

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+71

Accession Score Description
1 ASPA_PSEAE 83 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+72

Accession Score Description
1 FTSA_PSEAE 82 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

+73

Accession Score Description
1 SYP_PSEPK 80 Prolyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=proS PE=3 SV=1

+74

Accession Score Description
1 RS9_PSEE4 80 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+75

Accession Score Description
1 SODF_PSEAE 79 Superoxide dismutase [Fe] OS=Pseudomonas aeruginosa GN=sodB PE=3 SV=3

+76

Accession Score Description
1 RL29_PSE14 77 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+77

Accession Score Description
1 ASSY_PSEPG 77 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+78

Accession Score Description
1 RL7_PSEP1 77 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+79

Accession Score Description
1 RL28_PSEE4 77 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1

-80

Accession Score Description
1 PURA_PSEPG 75 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1
Score Mass Matches Sequences emPAI
80.1 PURA_PSEPG 75 47089 4 (2) 4 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1
4 samesets of PURA_PSEPG
PURA_PSEPK 75 47089 4 (2) 4 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain KT2440) GN=purA PE=3 SV=1
PURA_PSEP1 75 47089 4 (2) 4 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purA PE=3 SV=1
PURA_PSEE4 75 47045 4 (2) 4 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas entomophila (strain L48) GN=purA PE=3 SV=1
PURA_PSEPW 75 46962 3 (2) 3 (2) 0.10
Adenylosuccinate synthetase OS=Pseudomonas putida (strain W619) GN=purA PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1746   440.2821 1317.8245 1317.8132 8.52 0 48 0.00049 +1Score > 27 indicates identity U K.TVLHLIPSGILR.E
2141   502.9636 1505.8690 1505.8566 8.24 1 53 5.9e-005 +1Score > 33 indicates identity
Score > 23 indicates homology		 U K.IVDLLTEHAAAVVR.Y
2681   442.4900 1765.9309 1765.9210 5.59 1 4 21 +5Score > 38 indicates identity
Score > 30 indicates homology		 E.LVGAPIDIISTGPDRNE.T
3254   1058.0433 2114.0720 2114.0500 10.4 1 2 5 +9Score > 39 indicates identity
Score > 21 indicates homology		 U R.EGVQCLIGNGVVVAPDALMR.E + Deamidated (NQ); Oxidation (M)

2 subsets and intersections (123 subset proteins in total)

Score Mass Subset of
PURA_PSEF5 53 47146 80.1
Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=purA PE=3 SV=1
2 samesets of PURA_PSEF5
PURA_PSEFS 53 46877
Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain SBW25) GN=purA PE=3 SV=1
PURA_PSEPF 53 46858
Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=purA PE=3 SV=1
PURA2_PHOLL 48 47439 80.1
Adenylosuccinate synthetase 2 OS=Photorhabdus luminescens subsp. laumondii GN=purA2 PE=3 SV=1
+119 samesets of PURA2_PHOLL
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