MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)


Page: 1 2 3 4 5 6  24 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

-8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
Score Mass Matches Sequences emPAI
8.1 OTCC_PSEPK 407 38115 33 (23) 14 (10) 1.56
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-33 peptide matches (22 non-duplicate, 11 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
134   499.3258 498.3185 498.3166 3.94 0 46 0.0034 +1Score > 34 indicates identity U R.IAAPK.A
367   345.6849 689.3552 689.3530 3.20 0 35 0.34 +1Score > 43 indicates identity K.LGMDVR.I
753   436.2320 870.4494 870.4460 3.95 0 36 0.093 +1Score > 39 indicates identity M.AFNIHNR.N
774   879.4984 878.4911 878.4861 5.67 0 40 0.037 +1Score > 38 indicates identity U R.YLLDLSR.D
777   440.2534 878.4922 878.4861 6.95 0 29 0.44 +5Score > 38 indicates identity U R.YLLDLSR.D
864   916.5013 915.4940 915.4913 2.97 1 51 0.0087 +1Score > 43 indicates identity U R.ITLTEDPK.A
865 +1 458.7545 915.4944 915.4913 3.44 1 42 0.016 +1Score > 43 indicates identity
Score > 37 indicates homology
U R.ITLTEDPK.A
1108 +3 1015.6149 1014.6076 1014.5961 11.4 0 21 0.55 +1Score > 37 indicates identity
Score > 31 indicates homology
U K.AILVSTLADL.-
1196   530.7438 1059.4730 1059.4695 3.35 1 41 0.034 +1Score > 39 indicates identity U R.MYDAIEYR.G
1295   1118.6283 1117.6210 1117.6131 7.06 1 73 2.6e-005 +1Score > 39 indicates identity U K.GNNIALIFEK.T
1296 +1 559.8188 1117.6230 1117.6131 8.88 1 54 0.00074 +1Score > 39 indicates identity
Score > 36 indicates homology
U K.GNNIALIFEK.T
1501 +1 402.2043 1203.5911 1203.5884 2.23 1 49 0.00046 +1Score > 42 indicates identity
Score > 28 indicates homology
U K.YTGTEQQHLK.G
1502   602.8032 1203.5918 1203.5884 2.87 1 50 0.00054 +1Score > 42 indicates identity
Score > 30 indicates homology
U K.YTGTEQQHLK.G
1752   330.9321 1319.6993 1319.6946 3.57 1 56 0.00032 +1Score > 40 indicates identity
Score > 34 indicates homology
U R.NLLSLEHHTTR.E
1754 +2 440.9074 1319.7004 1319.6946 4.39 1 49 0.00044 +1Score > 40 indicates identity
Score > 28 indicates homology
U R.NLLSLEHHTTR.E
1755   660.8577 1319.7008 1319.6946 4.75 1 58 0.00029 +1Score > 40 indicates identity
Score > 36 indicates homology
U R.NLLSLEHHTTR.E
1811 +1 672.8756 1343.7366 1343.7231 10.1 0 68 8.7e-006 +1Score > 39 indicates identity
Score > 30 indicates homology
U R.NNMGNSLLLIGAK.L
1817 +2 450.9000 1349.6782 1349.6728 3.99 1 42 0.0016 +1Score > 41 indicates identity
Score > 27 indicates homology
U K.ALWPHDDLVER.C
1819   675.8475 1349.6804 1349.6728 5.67 1 34 0.016 +1Score > 41 indicates identity
Score > 28 indicates homology
U K.ALWPHDDLVER.C
2256   521.2881 1560.8425 1560.8334 5.84 1 25 0.072 +1Score > 39 indicates identity
Score > 27 indicates homology
U K.QLKPYQVNAELMK.S
4369   756.3902 3021.5317 3021.4729 19.5 1 11 0.25 +1Score > 36 indicates identity
Score > 18 indicates homology
U K.FAGVPVFNGLTDEYHPTQMIADVLTMR.E
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_5_20250714121805.raw

Score > 36 indicates identity

Score > 19 indicates homology

4370   1008.5087 3022.5043 3022.4569 15.7 1 58 7.1e-006 -1Score > 36 indicates identity
Score > 19 indicates homology
U K.FAGVPVFNGLTDEYHPTQMIADVLTMR.E + Deamidated (NQ)
15.7 1 58 7.1e-006 1 FAGVPVFNGLTDEYHPTQMLADVLTMR   + Deamidated (NQ)
15.7 1 46 9.9e-005 3 K.FAGVPVFNGLTDEYHPTQMIADVLTMR.E + Deamidated (NQ)
15.7 1 46 9.9e-005 3 FAGVPVFNGLTDEYHPTQMLADVLTMR   + Deamidated (NQ)
10.3 0 3 2.2 5 TLGVVYAPLQYPGGQTQLTNINAIMVQE   + 5 Deamidated (NQ)

9 subsets and intersections (45 subset proteins in total)

Score Mass Subset of
OTCC_PSEAE 201 38255 8.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas aeruginosa GN=arcB PE=1 SV=3
OTCC_PSEME 164 38189 8.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas mendocina GN=arcB PE=3 SV=3
OTCC_BURMA 91 38278 8.1
Ornithine carbamoyltransferase, catabolic OS=Burkholderia mallei GN=arcB PE=3 SV=1
3 samesets of OTCC_BURMA
OTCC_BURPS 91 38278
Ornithine carbamoyltransferase, catabolic OS=Burkholderia pseudomallei GN=arcB PE=3 SV=1
OTCC_HAEGA 91 37705
Ornithine carbamoyltransferase, catabolic OS=Haemophilus gallinarum GN=arcB PE=3 SV=1
OTC_HAEDU 91 37722
Ornithine carbamoyltransferase OS=Haemophilus ducreyi GN=argF PE=3 SV=1
OTCC_HAEIN 91 37908 8.1
Ornithine carbamoyltransferase, catabolic OS=Haemophilus influenzae GN=arcB PE=3 SV=1
1 sameset of OTCC_HAEIN
OTC_HAEIG 91 37881
Ornithine carbamoyltransferase OS=Haemophilus influenzae (strain PittGG) GN=arcB PE=3 SV=1
OTC_MANSM 88 37793 8.1
Ornithine carbamoyltransferase OS=Mannheimia succiniciproducens (strain MBEL55E) GN=argF PE=3 SV=1
OTCC_CHRVO 56 38025 8.1
Ornithine carbamoyltransferase, catabolic OS=Chromobacterium violaceum GN=arcB PE=3 SV=1
AMN1_PICAN 46 0 8.1
description
19 samesets of AMN1_PICAN
OTC_CLOAB 46 0
description
YHC3_SCHPO 46 0
description
SYA_IGNH4 46 0
description
CIPKB_ORYSJ 46 0
description
IML1_NEUCR 46 0
description
CHDM_DROME 46 0
description
FAS_RAT 46 0
description
GB_MCMVS 46 0
description
MURI_LACAC 46 0
description
THEG_HUMAN 46 0
description
BOP1_DICDI 46 0
description
DOP1_EMENI 46 0
description
DNAE2_RALPJ 46 0
description
DNAE2_RALSO 46 0
description
IMPA3_MOUSE 46 0
description
RL3_SPHWW 46 0
description
RS4_DROME 46 0
description
RL32_CARHZ 46 0
description
UNC53_CAEEL 46 0
description
OTCC_RHIET 41 38135 8.1
Ornithine carbamoyltransferase, catabolic OS=Rhizobium etli GN=arcB PE=3 SV=1
12 samesets of OTCC_RHIET
OTC_ACTPJ 41 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=arcB PE=3 SV=1
OTC_NEIGO 41 36823
Ornithine carbamoyltransferase OS=Neisseria gonorrhoeae GN=argF PE=3 SV=1
OTC_NEILA 41 28761
Ornithine carbamoyltransferase (Fragment) OS=Neisseria lactamica GN=argF PE=3 SV=1
OTC_NEIMA 41 36795
Ornithine carbamoyltransferase OS=Neisseria meningitidis serogroup A GN=argF PE=3 SV=1
OTC_NEIMB 41 36807
Ornithine carbamoyltransferase OS=Neisseria meningitidis serogroup B GN=argF PE=3 SV=1
OTC_NEIME 41 28974
Ornithine carbamoyltransferase (Fragment) OS=Neisseria meningitidis GN=argF PE=3 SV=2
OTC_NEIPE 41 25521
Ornithine carbamoyltransferase (Fragment) OS=Neisseria perflava GN=argF PE=3 SV=1
OTC_NEIPO 41 28876
Ornithine carbamoyltransferase (Fragment) OS=Neisseria polysaccharea GN=argF PE=3 SV=1
OTC_NEIG1 41 36768
Ornithine carbamoyltransferase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=argF PE=3 SV=1
OTC_ACTP2 41 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=argF PE=3 SV=1
OTC_ACTP7 41 37600
Ornithine carbamoyltransferase OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=arcB PE=3 SV=1
OTCC_RHIME 41 37978
Ornithine carbamoyltransferase, catabolic OS=Rhizobium meliloti GN=arcB PE=3 SV=1
OTCC_MYCCC 40 35232 8.1
Ornithine carbamoyltransferase, catabolic OS=Mycoplasma capricolum subsp. capripneumoniae GN=arcB PE=3 SV=1
1 sameset of OTCC_MYCCC
THI22_YEAST 40 64521
Thiamine biosynthesis protein THI22 OS=Saccharomyces cerevisiae GN=THI22 PE=2 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
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