MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 240)

Page: Previous 1 …3 4 5 6 7 8 9 10 11 12 13 … 24 Next 

+71

Accession Score Description
1 ASPA_PSEAE 83 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+72

Accession Score Description
1 FTSA_PSEAE 82 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

+73

Accession Score Description
1 SYP_PSEPK 80 Prolyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=proS PE=3 SV=1

+74

Accession Score Description
1 RS9_PSEE4 80 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+75

Accession Score Description
1 SODF_PSEAE 79 Superoxide dismutase [Fe] OS=Pseudomonas aeruginosa GN=sodB PE=3 SV=3

+76

Accession Score Description
1 RL29_PSE14 77 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+77

Accession Score Description
1 ASSY_PSEPG 77 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+78

Accession Score Description
1 RL7_PSEP1 77 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

-79

Accession Score Description
1 RL28_PSEE4 77 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1
Score Mass Matches Sequences emPAI
79.1 RL28_PSEE4 77 8988 2 (2) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1
3 samesets of RL28_PSEE4
RL28_PSEP1 77 8974 2 (2) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1
RL28_PSEPG 77 8974 2 (2) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas putida (strain GB-1) GN=rpmB PE=3 SV=1
RL28_PSEPK 77 8974 2 (2) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1212   535.8195 1069.6244 1069.6132 10.6 0 50 0.0031 +1Score > 38 indicates identity U R.GIDAVLVDIR.R
1968   474.9101 1421.7085 1421.7011 5.17 0 58 2.6e-005 +1Score > 41 indicates identity
Score > 25 indicates homology		 U K.GPVTGNNISHANNK.T

2 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
RL28_AZOVD 58 9074 79.1
50S ribosomal protein L28 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpmB PE=3 SV=1
9 samesets of RL28_AZOVD
RL28_PSE14 58 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmB PE=3 SV=1
RL28_PSEF5 58 9046
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpmB PE=3 SV=1
RL28_PSEFS 58 8902
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain SBW25) GN=rpmB PE=3 SV=1
RL28_PSEMY 58 9060
50S ribosomal protein L28 OS=Pseudomonas mendocina (strain ymp) GN=rpmB PE=3 SV=1
RL28_PSEPF 58 9003
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpmB PE=3 SV=1
RL28_PSEPW 58 9094
50S ribosomal protein L28 OS=Pseudomonas putida (strain W619) GN=rpmB PE=3 SV=1
RL28_PSESM 58 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. tomato GN=rpmB PE=3 SV=1
RL28_PSEU2 58 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpmB PE=3 SV=1
RL28_PSEU5 58 9060
50S ribosomal protein L28 OS=Pseudomonas stutzeri (strain A1501) GN=rpmB PE=3 SV=1
RL28_VIBSL 50 9081 79.1
50S ribosomal protein L28 OS=Vibrio splendidus (strain LGP32) GN=rpmB PE=3 SV=1

+80

Accession Score Description
1 PURA_PSEPG 75 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1
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