MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 240)


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+71

Accession Score Description
1 ASPA_PSEAE 83 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+72

Accession Score Description
1 FTSA_PSEAE 82 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

+73

Accession Score Description
1 SYP_PSEPK 80 Prolyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=proS PE=3 SV=1

-74

Accession Score Description
1 RS9_PSEE4 80 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
Score Mass Matches Sequences emPAI
74.1 RS9_PSEE4 80 14597 5 (4) 4 (3) 0.57
30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
3 samesets of RS9_PSEE4
RS9_PSEP1 80 14583 5 (4) 4 (3) 0.57
30S ribosomal protein S9 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsI PE=3 SV=1
RS9_PSEPG 80 14597 5 (4) 4 (3) 0.57
30S ribosomal protein S9 OS=Pseudomonas putida (strain GB-1) GN=rpsI PE=3 SV=1
RS9_PSEPK 80 14583 5 (4) 4 (3) 0.57
30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
310   333.6832 665.3518 665.3497 3.27 0 31 0.095 +1Score > 39 indicates identity
Score > 34 indicates homology
R.AGYVTR.D
924   470.7516 939.4886 939.4814 7.68 0 35 0.037 +1Score > 39 indicates identity
Score > 33 indicates homology
U R.SLDVFFGR.E
1182   527.7526 1053.4906 1053.4839 6.38 0 63 0.00027 +1Score > 40 indicates identity U M.SATQNYGTGR.R
2479 +1 553.3116 1656.9130 1656.9060 4.22 0 44 0.0011 +1Score > 37 indicates identity
Score > 27 indicates homology
U R.VFLRPGTGNISINNR.S

2 subsets and intersections (14 subset proteins in total)

Score Mass Subset of
RS9_PSE14 77 14593 74.1
30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1
6 samesets of RS9_PSE14
RS9_PSEMY 77 14637
30S ribosomal protein S9 OS=Pseudomonas mendocina (strain ymp) GN=rpsI PE=3 SV=1
RS9_PSEFS 77 14607
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsI PE=3 SV=1
RS9_PSESM 77 14593
30S ribosomal protein S9 OS=Pseudomonas syringae pv. tomato GN=rpsI PE=3 SV=1
RS9_PSEU2 77 14593
30S ribosomal protein S9 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsI PE=3 SV=1
RS9_PSEF5 77 14610
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsI PE=3 SV=1
RS9_PSEPF 77 14597
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsI PE=3 SV=1
RS9_AZOVD 63 14723 74.1
30S ribosomal protein S9 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsI PE=3 SV=1
6 samesets of RS9_AZOVD
RS9_PSEA7 63 14616
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsI PE=3 SV=1
RS9_PSEA8 63 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsI PE=3 SV=1
RS9_PSEAB 63 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsI PE=3 SV=1
RS9_PSEAE 63 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa GN=rpsI PE=3 SV=1
RS9_PSEPW 63 14581
30S ribosomal protein S9 OS=Pseudomonas putida (strain W619) GN=rpsI PE=3 SV=1
RS9_PSEU5 63 14604
30S ribosomal protein S9 OS=Pseudomonas stutzeri (strain A1501) GN=rpsI PE=3 SV=1

+75

Accession Score Description
1 SODF_PSEAE 79 Superoxide dismutase [Fe] OS=Pseudomonas aeruginosa GN=sodB PE=3 SV=3

+76

Accession Score Description
1 RL29_PSE14 77 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+77

Accession Score Description
1 ASSY_PSEPG 77 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+78

Accession Score Description
1 RL7_PSEP1 77 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+79

Accession Score Description
1 RL28_PSEE4 77 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1

+80

Accession Score Description
1 PURA_PSEPG 75 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1
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