MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 96 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 94 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP1_PSEAE | 92 | Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2 |
64| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPD_PSEPG | 89 | ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1 |
65| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACSA1_PSEPK | 87 | Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1 |
66| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KPRS_PSEPK | 86 | Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1 |
67| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CARB_PSEPK | 86 | Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2 |
68| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 85 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
69| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SECB_PSEPG | 85 | Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1 |
70| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 84 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 70.1 | NUOG_PSEPK |
84 | 98547 | 5 (4) | 5 (4) | 0.10 | |
| NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 222 |
616.3431 | 615.3358 | 615.3340 | 2.96 | 1 | 19 | 1.6 | 5Score > 44 indicates identity Score > 33 indicates homology |
E.AGELAR.V | ||
| 1118 |
508.8146 | 1015.6146 | 1015.6066 | 7.94 | 0 | 23 | 0.042 | 1Score > 34 indicates identity Score > 22 indicates homology |
U | R.IPAAYVALAK.S | |
| 2548 |
844.4484 | 1686.8822 | 1686.8630 | 11.4 | 0 | 52 | 0.00011 | 1Score > 40 indicates identity Score > 25 indicates homology |
U | R.LNWFNAIPGAFNPAR.G | |
| 3730 |
788.1302 | 2361.3688 | 2361.3267 | 17.8 | 1 | 42 | 0.0018 | 1Score > 27 indicates identity |
U | K.RPLVVAGTSLADPALIEAAANIAK.A + Deamidated (NQ) | |
| 3994 |
871.4304 | 2611.2694 | 2611.2496 | 7.58 | 1 | 43 | 0.0016 | 1Score > 38 indicates identity Score > 28 indicates homology |
U | R.FFQVFDPQYLDSSIQIHEGWR.W |
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