MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 240)

Page: Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 24 Next 

+61

Accession Score Description
1 ODB2_PSEPU 96 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+62

Accession Score Description
1 ACP_PSEPK 94 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+63

Accession Score Description
1 ODP1_PSEAE 92 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+64

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+65

Accession Score Description
1 ACSA1_PSEPK 87 Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1

+66

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+67

Accession Score Description
1 CARB_PSEPK 86 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

-68

Accession Score Description
1 IF2_PSEE4 85 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1
Score Mass Matches Sequences emPAI
68.1 IF2_PSEE4 85 91973 5 (1) 5 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1
17 samesets of IF2_PSEE4
IF2_CAMHC 85 100767 3 (1) 3 (1) 0.02
Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1
IF2_PSEP1 85 91620 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=infB PE=3 SV=1
IF2_PSEPG 85 91294 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain GB-1) GN=infB PE=3 SV=1
IF2_PSEPK 85 91576 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1
IF2_PSEPW 85 91289 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain W619) GN=infB PE=3 SV=1
IF2_CAMLR 85 96725 3 (1) 3 (1) 0.02
Translation initiation factor IF-2 OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=infB PE=3 SV=1
IF2_SYNE7 85 110858 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus elongatus (strain PCC 7942) GN=infB PE=3 SV=1
IF2_SYNP6 85 110787 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=infB PE=3 SV=1
IF2_MICAN 85 109614 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Microcystis aeruginosa (strain NIES-843) GN=infB PE=3 SV=1
IF2_CYAP7 85 121749 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7424) GN=infB PE=3 SV=1
IF2_SYNP2 85 105884 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=infB PE=3 SV=1
IF2_SYNJA 85 111852 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain JA-3-3Ab) GN=infB PE=3 SV=1
IF2_CYAP4 85 108559 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=infB PE=3 SV=1
IF2_SYNJB 85 113485 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. GN=infB PE=3 SV=1
IF2_SYNPW 85 119022 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain WH7803) GN=infB PE=3 SV=1
IF2_SYNS3 85 118793 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain CC9311) GN=infB PE=3 SV=1
IF2_THEEB 85 104469 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Thermosynechococcus elongatus (strain BP-1) GN=infB PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1032   490.7826 979.5506 979.5338 17.2 0 25 0.37 +1Score > 38 indicates identity
Score > 33 indicates homology		 K.TSLLDYIR.R
1349   569.3572 1136.6998 1136.6917 7.13 0 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology		 K.AAGVPLVVAVNK.I
2007   720.4234 1438.8322 1438.8144 12.4 0 85 5.9e-007 +1Score > 35 indicates identity U R.GPVATILVQDGTLR.Q
2263   522.2737 1563.7993 1563.7906 5.52 0 16 0.52 +1Score > 41 indicates identity
Score > 26 indicates homology		 U R.NQHGFQNPTGPVIR.D
3305   1068.0332 2134.0518 2134.0828 -14.5 1 3 1.1 +2Score > 40 indicates identity
Score > 15 indicates homology		 U R.DVTIGETITVSDLAQQMSVK.G

+69

Accession Score Description
1 SECB_PSEPG 85 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+70

Accession Score Description
1 NUOG_PSEPK 84 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1
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