MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 240)


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+61

Accession Score Description
1 ODB2_PSEPU 96 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+62

Accession Score Description
1 ACP_PSEPK 94 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+63

Accession Score Description
1 ODP1_PSEAE 92 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+64

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+65

Accession Score Description
1 ACSA1_PSEPK 87 Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1

-66

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1
Score Mass Matches Sequences emPAI
66.1 KPRS_PSEPK 86 34456 4 (3) 4 (3) 0.21
Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1
1 sameset of KPRS_PSEPK
KPRS_PSESM 86 34412 4 (3) 4 (3) 0.21
Ribose-phosphate pyrophosphokinase OS=Pseudomonas syringae pv. tomato GN=prs PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1320   565.3323 1128.6500 1128.6390 9.76 0 22 0.072 +1Score > 39 indicates identity
Score > 23 indicates homology
U K.SLGVDLGIIDK.R
1845   454.9333 1361.7781 1361.7667 8.35 0 59 0.00017 +1Score > 35 indicates identity
Score > 34 indicates homology
U R.QLHIPLGDVSVGK.F
1858   685.8868 1369.7590 1369.7394 14.3 0 45 0.0056 +1Score > 39 indicates identity
Score > 35 indicates homology
U R.ITAVIPYFGYAR.Q
1872   690.9056 1379.7966 1379.7773 14.1 1 51 0.0011 +1Score > 34 indicates identity U R.QLDIAPVVAEAVR.R

2 subsets and intersections (30 subset proteins in total)

Score Mass Subset of
KPRS_PSEAE 69 34247 66.1
Ribose-phosphate pyrophosphokinase OS=Pseudomonas aeruginosa GN=prs PE=3 SV=1
KPRS_AGRT5 45 33550 66.1
Ribose-phosphate pyrophosphokinase OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970) GN=prs PE=3 SV=2
+28 samesets of KPRS_AGRT5

+67

Accession Score Description
1 CARB_PSEPK 86 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+68

Accession Score Description
1 IF2_PSEE4 85 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+69

Accession Score Description
1 SECB_PSEPG 85 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+70

Accession Score Description
1 NUOG_PSEPK 84 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1
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