MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 240)

Page: Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 24 Next 

+61

Accession Score Description
1 ODB2_PSEPU 96 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+62

Accession Score Description
1 ACP_PSEPK 94 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+63

Accession Score Description
1 ODP1_PSEAE 92 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+64

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

-65

Accession Score Description
1 ACSA1_PSEPK 87 Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1
Score Mass Matches Sequences emPAI
65.1 ACSA1_PSEPK 87 72208 5 (3) 5 (3) 0.10
Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1192   529.7973 1057.5800 1057.5768 3.09 1 17 1.1 +4Score > 41 indicates identity
Score > 30 indicates homology		 K.VVITADEGVR.G
1375   383.2243 1146.6511 1146.6437 6.41 0 27 0.092 +1Score > 38 indicates identity
Score > 29 indicates homology		 R.IDWIKPFTK.V
1784   666.3959 1330.7772 1330.7609 12.3 0 50 0.00045 +1Score > 34 indicates identity
Score > 29 indicates homology		 U K.VNILYTAPTAIR.A
3050   662.3658 1984.0756 1984.0531 11.3 1 37 0.05 +1Score > 37 indicates identity U R.IGAIHSVVFGGFSPEALAGR.I
3523   1119.0698 2236.1250 2236.0840 18.3 1 71 3.6e-005 +1Score > 39 indicates identity U R.LLGSVGEPINPEAWNWYYK.T + Deamidated (NQ)

3 subsets and intersections (17 subset proteins in total)

Score Mass Subset of
ACSA1_PSEAE 54 72230 65.1
Acetyl-coenzyme A synthetase 1 OS=Pseudomonas aeruginosa GN=acsA1 PE=3 SV=1
4 samesets of ACSA1_PSEAE
ACSA_PSEU5 54 72236
Acetyl-coenzyme A synthetase OS=Pseudomonas stutzeri (strain A1501) GN=acsA PE=3 SV=1
ACSA_PSESM 54 71952
Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. tomato GN=acsA PE=3 SV=1
ACSA_PSEU2 54 71965
Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=acsA PE=3 SV=1
ACSA_PSE14 54 71908
Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=acsA PE=3 SV=1
ACSA_ENTS8 50 72106 65.1
Acetyl-coenzyme A synthetase OS=Enterobacter sakazakii (strain ATCC BAA-894) GN=acs PE=3 SV=1
9 samesets of ACSA_ENTS8
ACSA_MARAV 50 72318
Acetyl-coenzyme A synthetase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=acsA PE=3 SV=1
ACSA_YERPS 50 72425
Acetyl-coenzyme A synthetase OS=Yersinia pseudotuberculosis GN=acs PE=3 SV=1
ACSA_YERP3 50 72425
Acetyl-coenzyme A synthetase OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=acs PE=3 SV=1
ACSA_YERPA 50 72425
Acetyl-coenzyme A synthetase OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=acs PE=3 SV=1
ACSA_YERPE 50 72425
Acetyl-coenzyme A synthetase OS=Yersinia pestis GN=acs PE=3 SV=2
ACSA_YERPN 50 72425
Acetyl-coenzyme A synthetase OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=acs PE=3 SV=1
ACSA_YERPP 50 72425
Acetyl-coenzyme A synthetase OS=Yersinia pestis (strain Pestoides F) GN=acs PE=3 SV=1
ACSA_PSEA6 50 71866
Acetyl-coenzyme A synthetase OS=Pseudoalteromonas atlantica (strain T6c / BAA-1087) GN=acsA PE=3 SV=1
ACSA_VIBCH 50 72248
Acetyl-coenzyme A synthetase OS=Vibrio cholerae GN=acsA PE=3 SV=2
ACSA2_RHIME 37 72604 65.1
Acetyl-coenzyme A synthetase 2 OS=Rhizobium meliloti GN=acsA2 PE=3 SV=1
1 sameset of ACSA2_RHIME
ACSA_RHISN 37 72845
Acetyl-coenzyme A synthetase OS=Rhizobium sp. (strain NGR234) GN=acsA PE=3 SV=1

+66

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+67

Accession Score Description
1 CARB_PSEPK 86 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+68

Accession Score Description
1 IF2_PSEE4 85 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+69

Accession Score Description
1 SECB_PSEPG 85 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+70

Accession Score Description
1 NUOG_PSEPK 84 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1
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