MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 240)


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+61

Accession Score Description
1 ODB2_PSEPU 96 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+62

Accession Score Description
1 ACP_PSEPK 94 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+63

Accession Score Description
1 ODP1_PSEAE 92 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

-64

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1
Score Mass Matches Sequences emPAI
64.1 ATPD_PSEPG 89 19242 4 (2) 4 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1
3 samesets of ATPD_PSEPG
ATPD_PSEPK 89 19242 4 (2) 4 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain KT2440) GN=atpH PE=3 SV=1
ATPD_PSEPW 89 19288 4 (2) 4 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain W619) GN=atpH PE=3 SV=1
ATPD_PSEP1 89 19242 4 (2) 4 (2) 0.26
ATP synthase subunit delta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpH PE=3 SV=1

+4 peptide matches (4 non-duplicate, 0 duplicate)

1 subset or intersection (20 subset proteins in total)

Score Mass Subset of
ATPD_AERS4 52 19175 64.1
ATP synthase subunit delta OS=Aeromonas salmonicida (strain A449) GN=atpH PE=3 SV=1
19 samesets of ATPD_AERS4
ATPD_PSEE4 52 19158
ATP synthase subunit delta OS=Pseudomonas entomophila (strain L48) GN=atpH PE=3 SV=1
ATPD_PSEAB 52 19305
ATP synthase subunit delta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpH PE=3 SV=1
ATPD_PSEAE 52 19305
ATP synthase subunit delta OS=Pseudomonas aeruginosa GN=atpH PE=3 SV=1
ATPD_PSESM 52 19372
ATP synthase subunit delta OS=Pseudomonas syringae pv. tomato GN=atpH PE=3 SV=1
ATPD_SHEON 52 19277
ATP synthase subunit delta OS=Shewanella oneidensis GN=atpH PE=3 SV=1
ATPD_SHESA 52 19251
ATP synthase subunit delta OS=Shewanella sp. (strain ANA-3) GN=atpH PE=3 SV=1
ATPD_SHESM 52 19250
ATP synthase subunit delta OS=Shewanella sp. (strain MR-4) GN=atpH PE=3 SV=1
ATPD_SHESR 52 19251
ATP synthase subunit delta OS=Shewanella sp. (strain MR-7) GN=atpH PE=3 SV=1
ATPD_PSEU2 52 19386
ATP synthase subunit delta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpH PE=3 SV=1
ATPD_PSEA7 52 19305
ATP synthase subunit delta OS=Pseudomonas aeruginosa (strain PA7) GN=atpH PE=3 SV=1
ATPD_PSEA8 52 19305
ATP synthase subunit delta OS=Pseudomonas aeruginosa (strain LESB58) GN=atpH PE=3 SV=1
ATPD_PSE14 52 19386
ATP synthase subunit delta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpH PE=3 SV=1
ATPD_LEGPA 52 19962
ATP synthase subunit delta OS=Legionella pneumophila (strain Paris) GN=atpH PE=3 SV=1
ATPD_LEGPC 52 19962
ATP synthase subunit delta OS=Legionella pneumophila (strain Corby) GN=atpH PE=3 SV=1
ATPD_LEGPH 52 19949
ATP synthase subunit delta OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) GN=atpH PE=3 SV=2
ATPD_LEGPL 52 19962
ATP synthase subunit delta OS=Legionella pneumophila (strain Lens) GN=atpH PE=3 SV=1
ATPD_PROMH 52 19620
ATP synthase subunit delta OS=Proteus mirabilis (strain HI4320) GN=atpH PE=3 SV=1
ATPD_PSEMY 52 19006
ATP synthase subunit delta OS=Pseudomonas mendocina (strain ymp) GN=atpH PE=3 SV=1
ATPD_THISH 52 19139
ATP synthase subunit delta OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=atpH PE=3 SV=1

+65

Accession Score Description
1 ACSA1_PSEPK 87 Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1

+66

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+67

Accession Score Description
1 CARB_PSEPK 86 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+68

Accession Score Description
1 IF2_PSEE4 85 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+69

Accession Score Description
1 SECB_PSEPG 85 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+70

Accession Score Description
1 NUOG_PSEPK 84 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1
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