MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 240)

Page: Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 24 Next 

+61

Accession Score Description
1 ODB2_PSEPU 96 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+62

Accession Score Description
1 ACP_PSEPK 94 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

-63

Accession Score Description
1 ODP1_PSEAE 92 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2
Score Mass Matches Sequences emPAI
63.1 ODP1_PSEAE 92 99843 5 (4) 4 (3) 0.09
Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
291   326.1778 650.3410 650.3387 3.54 1 19 0.61 +1Score > 41 indicates identity
Score > 30 indicates homology		 K.YLAER.R
910   466.2657 930.5168 930.5134 3.66 1 46 0.02 +1Score > 42 indicates identity U K.KVDVDSLR.A
1346   379.8728 1136.5966 1136.5938 2.43 1 36 0.0081 +1Score > 40 indicates identity
Score > 27 indicates homology		 U R.NTIPVTHEAR.M
1347   569.3069 1136.5992 1136.5938 4.78 1 44 0.0034 +1Score > 40 indicates identity
Score > 32 indicates homology		 U R.NTIPVTHEAR.M
1571   616.3085 1230.6024 1230.5993 2.57 0 67 2.9e-005 +1Score > 41 indicates identity
Score > 35 indicates homology		 U R.IGDLAWAAGDSR.A

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
SPRT_VIBSL 46 19585 63.1
Protein sprT OS=Vibrio splendidus (strain LGP32) GN=sprT PE=3 SV=1

+64

Accession Score Description
1 ATPD_PSEPG 89 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+65

Accession Score Description
1 ACSA1_PSEPK 87 Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain KT2440) GN=acsA1 PE=3 SV=1

+66

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+67

Accession Score Description
1 CARB_PSEPK 86 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

+68

Accession Score Description
1 IF2_PSEE4 85 Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1

+69

Accession Score Description
1 SECB_PSEPG 85 Protein-export protein secB OS=Pseudomonas putida (strain GB-1) GN=secB PE=3 SV=1

+70

Accession Score Description
1 NUOG_PSEPK 84 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1
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