MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 240)

Page: Previous 1 2 3 4 5 6 7 8 9 10 11 … 24 Next 

+51

Accession Score Description
1 CLPB_PSEPK 107 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+52

Accession Score Description
1 DHSB_ECOLI 103 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+53

Accession Score Description
1 ODO1_AZOVI 101 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+54

Accession Score Description
1 RS11_AZOVD 101 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+55

Accession Score Description
1 YEAG_ECOLI 100 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+56

Accession Score Description
1 FLAE_VIBAN 100 Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1

+57

Accession Score Description
1 ASPQ_PSEPK 100 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+58

Accession Score Description
1 SYA_PSEPG 98 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+59

Accession Score Description
Family member distances as a dendrogram 1 EFTS_PSEPG 96 Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1
2 CLPX_AZOSB 84 ATP-dependent Clp protease ATP-binding subunit clpX OS=Azoarcus sp. (strain BH72) GN=clpX PE=3 SV=1

-60

Accession Score Description
1 CH10_PSEP1 96 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1
Score Mass Matches Sequences emPAI
60.1 CH10_PSEP1 96 10217 7 (3) 5 (3) 0.90
10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1
7 samesets of CH10_PSEP1
CH10_PSEPK 96 10217 7 (3) 5 (3) 0.90
10 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groS PE=3 SV=1
CH10_PSEST 96 10261 7 (3) 5 (3) 0.90
10 kDa chaperonin OS=Pseudomonas stutzeri GN=groS PE=3 SV=1
CH10_PSEU5 96 10259 7 (3) 5 (3) 0.90
10 kDa chaperonin OS=Pseudomonas stutzeri (strain A1501) GN=groS PE=3 SV=1
CH10_PSE14 96 10261 6 (3) 4 (3) 0.90
10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1
CH10_PSESM 96 10261 6 (3) 4 (3) 0.90
10 kDa chaperonin OS=Pseudomonas syringae pv. tomato GN=groS PE=3 SV=1
CH10_PSEU2 96 10261 6 (3) 4 (3) 0.90
10 kDa chaperonin OS=Pseudomonas syringae pv. syringae (strain B728a) GN=groS PE=3 SV=1
CH10_PSEF5 96 10245 6 (3) 4 (3) 0.90
10 kDa chaperonin OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=groS PE=3 SV=1

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
830 +1 451.2419 900.4692 900.4665 3.06 1 42 0.053 +1Score > 42 indicates identity
Score > 41 indicates homology		 U R.VLDNGEVR.A
841   302.8481 905.5225 905.5195 3.26 0 25 1.1 +6Score > 38 indicates identity K.LRPLHDR.V
842   453.7691 905.5236 905.5195 4.56 0 14 2.7 +4Score > 38 indicates identity
Score > 31 indicates homology		 K.LRPLHDR.V
937   472.7635 943.5124 943.5087 3.96 1 40 0.07 +1Score > 42 indicates identity
Score > 41 indicates homology		 U R.GEVVAVGTGR.V
1831   677.8575 1353.7004 1353.6929 5.59 0 67 9.7e-006 +1Score > 40 indicates identity
Score > 29 indicates homology		 U K.VVFGPYSGSNTVK.V
2449   546.6397 1636.8973 1636.8896 4.66 1 57 0.00024 +1Score > 37 indicates identity
Score > 34 indicates homology		 U K.TAGGIVLPGSAAEKPNR.G

4 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
CH10_PSEE4 92 10245 60.1
10 kDa chaperonin OS=Pseudomonas entomophila (strain L48) GN=groS PE=3 SV=1
3 samesets of CH10_PSEE4
CH10_PSEPG 92 10260
10 kDa chaperonin OS=Pseudomonas putida (strain GB-1) GN=groS PE=3 SV=1
CH10_PSEPW 92 10247
10 kDa chaperonin OS=Pseudomonas putida (strain W619) GN=groS PE=3 SV=1
CH10_PSEPU 92 10274
10 kDa chaperonin OS=Pseudomonas putida GN=groS PE=3 SV=1
CH10_PSEMY 67 10229 60.1
10 kDa chaperonin OS=Pseudomonas mendocina (strain ymp) GN=groS PE=3 SV=1
1 sameset of CH10_PSEMY
CH10_PSEPF 67 10214
10 kDa chaperonin OS=Pseudomonas fluorescens (strain Pf0-1) GN=groS PE=3 SV=1
CH10_PSEA7 61 10260 60.1
10 kDa chaperonin OS=Pseudomonas aeruginosa (strain PA7) GN=groS PE=3 SV=1
3 samesets of CH10_PSEA7
CH10_PSEA8 61 10260
10 kDa chaperonin OS=Pseudomonas aeruginosa (strain LESB58) GN=groS PE=3 SV=1
CH10_PSEAB 61 10260
10 kDa chaperonin OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=groS PE=3 SV=1
CH10_PSEAE 61 10260
10 kDa chaperonin OS=Pseudomonas aeruginosa GN=groS PE=3 SV=1
CH10_AZOVD 57 10305 60.1
10 kDa chaperonin OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=groS PE=3 SV=1
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