MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1313 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 3 | EFTU_CARRP | 105 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1215 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 945 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_PSEU5 | 708 | ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1 | |
| 4 | ATPB_LEGPA | 195 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
| 3 | ATPB_PSE14 | 666 | ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 849 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 2 | CH602_SORC5 | 95 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 777 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 168 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_VEREI | 155 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 648 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 454 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | RL1_PSEE4 |
454 | 24208 | 13 (11) | 6 (6) | 1.08 | |
| 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 | |||||||
| 2 samesets of RL1_PSEE4 | |||||||
| RL1_PSEP1 |
454 | 24236 | 13 (11) | 6 (6) | 1.08 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplA PE=3 SV=1 | |||||||
| RL1_PSEPK |
454 | 24236 | 13 (11) | 6 (6) | 1.08 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 | |||||||
13 peptide matches (10 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1132 |
342.5306 | 1024.5700 | 1024.5665 | 3.35 | 0 | 37 | 0.011 | 1Score > 37 indicates identity Score > 30 indicates homology |
U | K.NGIIHTSVGK.V | |
| 1133 |
513.2931 | 1024.5716 | 1024.5665 | 4.98 | 0 | 62 | 0.00019 | 1Score > 38 indicates identity Score > 37 indicates homology |
U | K.NGIIHTSVGK.V | |
| 1206 |
356.5346 | 1066.5820 | 1066.5771 | 4.55 | 0 | 29 | 0.12 | 1Score > 38 indicates identity Score > 32 indicates homology |
U | R.SATVLPHGTGK.T | |
| 1207 |
534.2983 | 1066.5820 | 1066.5771 | 4.62 | 0 | 54 | 0.0014 | 1Score > 38 indicates identity |
U | R.SATVLPHGTGK.T | |
| 1549 |
1 |
611.8707 | 1221.7268 | 1221.7194 | 6.12 | 0 | 67 | 3.8e-006 | 1Score > 32 indicates identity Score > 25 indicates homology |
U | R.VVGQLGQVLGPR.G |
| 2735 |
897.4382 | 1792.8618 | 1792.8302 | 17.7 | 0 | 63 | 4e-005 | 1Score > 40 indicates identity Score > 31 indicates homology |
U | K.GGDLNYDVVIASPDAMR.V + Deamidated (NQ) | |
| 2737 |
1 |
897.4694 | 1792.9242 | 1792.8996 | 13.8 | 1 | 45 | 0.00074 | 1Score > 40 indicates identity Score > 26 indicates homology |
U | K.FVESYDIAVNLGVDPR.K |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_5_20250714121805.rawScore > 40 indicates identity Score > 19 indicates homology |
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| 2738 |
598.6488 | 1792.9246 | 1792.8996 | 13.9 | 1 | 17 | 0.1 | 1Score > 40 indicates identity Score > 19 indicates homology |
U | K.FVESYDIAVNLGVDPR.K | |
| -12.6 | 1 | 6 | 1.3 | 2 | WLLGQEIVTATAHTPR + Deamidated (NQ) | ||||||
| -6.46 | 1 | 5 | 1.4 | 3 | MISRLTSLTCVPMLR + Oxidation (M) | ||||||
| -4.10 | 0 | 5 | 1.5 | 4 | DLQPGAAASAVLVDGGVPR + Deamidated (NQ) | ||||||
| 12.1 | 0 | 5 | 1.7 | 5 | IGTVNPLASTSLNACFK + Deamidated (NQ) | ||||||
| 13.9 | 1 | 4 | 2 | 6 | FTESFDIAINLGVDPR | ||||||
| 16.2 | 1 | 3 | 2.2 | 7 | DAQAAEIPIPSVNTPNR + Deamidated (NQ) | ||||||
| 5.45 | 0 | 3 | 2.4 | 8 | APNFQIHVLSSLFYR + 2 Deamidated (NQ) | ||||||
| 2.16 | 1 | 2 | 2.9 | 9 | DGVLKVGDAVVASNTYGK + Deamidated (NQ) | ||||||
| 3.66 | 1 | 2 | 3 | 10 | GVDGIVLSNHGGRQLDR + Deamidated (NQ) | ||||||
| 2896 |
629.3364 | 1884.9874 | 1884.9694 | 9.56 | 1 | 70 | 3.6e-006 | 1Score > 39 indicates identity Score > 28 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V | |
| 2899 |
1 |
944.0003 | 1885.9860 | 1885.9534 | 17.3 | 1 | 135 | 2.1e-012 | 1Score > 39 indicates identity Score > 31 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL1_PSEPG |
422 | 24180 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain GB-1) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEF5 |
413 | 24047 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEF5 | |||||
| RL1_PSEPW |
413 | 24112 | |||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain W619) GN=rplA PE=3 SV=1 | |||||
| RL1_PSE14 |
382 | 24120 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplA PE=3 SV=1 | |||||
| 2 samesets of RL1_PSE14 | |||||
| RL1_PSESM |
382 | 24163 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1 | |||||
| RL1_PSEU2 |
382 | 24176 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEFS |
355 | 24061 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain SBW25) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEFS | |||||
| RL1_PSEPF |
355 | 24193 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplA PE=3 SV=1 | |||||
| RL1_AZOVD |
305 | 24230 | 6.1 | ||
| 50S ribosomal protein L1 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEU5 |
153 | 24129 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas stutzeri (strain A1501) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEMY |
121 | 23957 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas mendocina (strain ymp) GN=rplA PE=3 SV=1 | |||||
| RL1_ACTP2 |
84 | 23932 | 6.1 | ||
| 50S ribosomal protein L1 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplA PE=3 SV=1 | |||||
| 58 samesets of RL1_ACTP2 |
|||||
| RL1_PSEA7 |
63 | 24219 | 6.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas aeruginosa (strain PA7) GN=rplA PE=3 SV=1 | |||||
| 3 samesets of RL1_PSEA7 | |||||
| RL1_PSEA8 |
63 | 24219 | |||
| 50S ribosomal protein L1 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEAB |
63 | 24219 | |||
| 50S ribosomal protein L1 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEAE |
63 | 24219 | |||
| 50S ribosomal protein L1 OS=Pseudomonas aeruginosa GN=rplA PE=3 SV=1 | |||||
| RL1_COXB1 |
40 | 24793 | 6.1 | ||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain CbuK_Q154) GN=rplA PE=3 SV=1 | |||||
| 4 samesets of RL1_COXB1 | |||||
| RL1_COXB2 |
40 | 24793 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain CbuG_Q212) GN=rplA PE=3 SV=1 | |||||
| RL1_COXBN |
40 | 24793 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rplA PE=3 SV=1 | |||||
| RL1_COXBR |
40 | 24823 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=rplA PE=3 SV=1 | |||||
| RL1_COXBU |
40 | 24823 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii GN=rplA PE=3 SV=1 | |||||
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 447 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 407 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 398 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 | |
| 6 | RPOB_CHRSD | 115 | DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1 | |
| 3 | RPOB_SACD2 | 141 | DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1 | |
| 5 | FTSZ_PSEPK | 123 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
| 2 | RPOC_PSEE4 | 267 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1 | |
| 7 | RPOC_COXBN | 77 | DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1 | |
| 4 | RPOC_SYNAS | 131 | DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEP1 | 340 | Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1 |
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