MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 107 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSB_ECOLI | 103 | Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO1_AZOVI | 101 | 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_AZOVD | 101 | 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 100 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FLAE_VIBAN | 100 | Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ASPQ_PSEPK | 100 | Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 98 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 58.1 | SYA_PSEPG |
98 | 95050 | 5 (2) | 5 (2) | 0.05 | |
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 | |||||||
| 3 samesets of SYA_PSEPG | |||||||
| SYA_PSEPK |
98 | 95109 | 5 (2) | 5 (2) | 0.05 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1 | |||||||
| SYA_PSEP1 |
98 | 95108 | 5 (2) | 5 (2) | 0.05 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2 | |||||||
| SYA_PSEE4 |
98 | 95309 | 3 (2) | 3 (2) | 0.05 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas entomophila (strain L48) GN=alaS PE=3 SV=1 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 148 |
529.3481 | 528.3408 | 528.3384 | 4.66 | 0 | 6 | 1.1 | 5Score > 41 indicates identity Score > 19 indicates homology |
K.VLAAR.L | ||
| 436 |
360.7378 | 719.4610 | 719.4581 | 4.06 | 0 | 19 | 0.54 | 1Score > 29 indicates identity Score > 28 indicates homology |
K.IIALYK.D | ||
| 1234 |
542.3484 | 1082.6822 | 1082.6699 | 11.4 | 0 | 36 | 0.023 | 1Score > 32 indicates identity |
U | K.ALLALVDQLK.N | |
| 2326 |
794.8985 | 1587.7824 | 1587.7740 | 5.32 | 1 | 92 | 1.9e-008 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | K.AASAAGDDLSNAAVEVK.G | |
| 4225 |
713.8923 | 2851.5401 | 2851.4868 | 18.7 | 1 | 18 | 1 | 1Score > 33 indicates identity Score > 30 indicates homology |
U | R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SYA_PSE14 |
36 | 94784 | 58.1 | ||
| Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=alaS PE=3 SV=1 | |||||
| 10 samesets of SYA_PSE14 | |||||
| SYA_PSEMY |
36 | 94797 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas mendocina (strain ymp) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEAB |
36 | 95112 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=alaS PE=3 SV=1 | |||||
| SYA_PSEAE |
36 | 95099 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=alaS PE=3 SV=1 | |||||
| SYA_PSEPW |
36 | 94991 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas putida (strain W619) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEF5 |
36 | 95022 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=alaS PE=3 SV=1 | |||||
| SYA_PSEU5 |
36 | 95029 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas stutzeri (strain A1501) GN=alaS PE=3 SV=2 | |||||
| SYA_PSEA7 |
36 | 94997 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain PA7) GN=alaS PE=3 SV=1 | |||||
| SYA_PSEPF |
36 | 94840 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=alaS PE=3 SV=1 | |||||
| SYA_PSESM |
36 | 94865 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=alaS PE=3 SV=1 | |||||
| SYA_PSEU2 |
36 | 94751 | |||
| Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=alaS PE=3 SV=2 | |||||
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPG | 96 | Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1 | |
| 2 | CLPX_AZOSB | 84 | ATP-dependent Clp protease ATP-binding subunit clpX OS=Azoarcus sp. (strain BH72) GN=clpX PE=3 SV=1 | |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH10_PSEP1 | 96 | 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1 |
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