MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 240)

Page: Previous 1 2 3 4 5 6 7 8 9 10 11 … 24 Next 

+51

Accession Score Description
1 CLPB_PSEPK 107 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+52

Accession Score Description
1 DHSB_ECOLI 103 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+53

Accession Score Description
1 ODO1_AZOVI 101 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+54

Accession Score Description
1 RS11_AZOVD 101 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+55

Accession Score Description
1 YEAG_ECOLI 100 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+56

Accession Score Description
1 FLAE_VIBAN 100 Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1

-57

Accession Score Description
1 ASPQ_PSEPK 100 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1
Score Mass Matches Sequences emPAI
57.1 ASPQ_PSEPK 100 38584 6 (4) 6 (4) 0.26
Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

-6 peptide matches (6 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
602   400.2443 798.4740 798.4712 3.60 0 6 1.3 +3Score > 38 indicates identity
Score > 19 indicates homology		 U K.NGVQIIR.S
1131   512.8145 1023.6144 1023.6077 6.63 1 23 0.09 +1Score > 34 indicates identity
Score > 25 indicates homology		 R.VVPALQELR.K
1477   595.2972 1188.5798 1188.5775 1.98 1 48 0.013 +1Score > 42 indicates identity U R.HTVNSEFDIK.Q
1980   476.2533 1425.7381 1425.7324 3.95 0 36 0.004 +1Score > 40 indicates identity
Score > 24 indicates homology		 U K.ALIHAGTGNGSVSSR.V
2231   775.9572 1549.8998 1549.8828 11.0 1 59 0.00013 +1Score > 33 indicates identity
Score > 32 indicates homology		 U K.LIAGVPELADIANVR.G
3889   835.4268 2503.2586 2503.2118 18.7 0 43 0.00013 +1Score > 38 indicates identity
Score > 17 indicates homology		 U K.QISSLPQVDIAYGYGNVTDTAYK.A + Deamidated (NQ)

2 subsets and intersections (2 subset proteins in total)

Score Mass Subset of
ASPQ_PSES7 77 36178 57.1
Glutaminase-asparaginase OS=Pseudomonas sp. (strain 7A) GN=ansB PE=1 SV=2
ASPQ_PSEAE 48 38620 57.1
Glutaminase-asparaginase OS=Pseudomonas aeruginosa GN=ansB PE=1 SV=1

+58

Accession Score Description
1 SYA_PSEPG 98 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+59

Accession Score Description
Family member distances as a dendrogram 1 EFTS_PSEPG 96 Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1
2 CLPX_AZOSB 84 ATP-dependent Clp protease ATP-binding subunit clpX OS=Azoarcus sp. (strain BH72) GN=clpX PE=3 SV=1

+60

Accession Score Description
1 CH10_PSEP1 96 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1
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