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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 240)

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+51

Accession Score Description
1 CLPB_PSEPK 107 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+52

Accession Score Description
1 DHSB_ECOLI 103 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+53

Accession Score Description
1 ODO1_AZOVI 101 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+54

Accession Score Description
1 RS11_AZOVD 101 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+55

Accession Score Description
1 YEAG_ECOLI 100 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

-56

Accession Score Description
1 FLAE_VIBAN 100 Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1
Score Mass Matches Sequences emPAI
56.1 FLAE_VIBAN 100 40757 3 (2) 3 (2) 0.12
Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1
15 samesets of FLAE_VIBAN
FLAA_VIBPA 100 39825 5 (2) 4 (2) 0.12
Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
FLAA_VIBAN 100 40087 2 (2) 2 (2) 0.12
Flagellin A OS=Vibrio anguillarum GN=flaA PE=3 SV=1
FLAA_VIBCH 100 40358 2 (2) 2 (2) 0.12
Flagellin A OS=Vibrio cholerae GN=flaA PE=3 SV=1
FLAB_VIBPA 100 40149 2 (2) 2 (2) 0.12
Polar flagellin B/D OS=Vibrio parahaemolyticus GN=flaB PE=3 SV=2
FLAC_VIBPA 100 40787 2 (2) 2 (2) 0.12
Polar flagellin C OS=Vibrio parahaemolyticus GN=flaC PE=3 SV=2
FLAD_VIBAN 100 39617 2 (2) 2 (2) 0.12
Flagellin D OS=Vibrio anguillarum GN=flaD PE=1 SV=3
FLAE_VIBCH 100 41017 2 (2) 2 (2) 0.12
Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1
FLAF_VIBPA 100 40476 2 (2) 2 (2) 0.12
Polar flagellin F OS=Vibrio parahaemolyticus GN=flaF PE=3 SV=2
FLICB_PSEAE 100 49213 2 (2) 2 (2) 0.10
B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2
FLAA_VIBC3 100 40358 2 (2) 2 (2) 0.12
Flagellin A OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaA PE=3 SV=1
FLAE_VIBC3 100 41017 2 (2) 2 (2) 0.12
Flagellin E OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaE PE=3 SV=1
FLAC_VIBCH 100 39881 2 (2) 2 (2) 0.12
Flagellin C OS=Vibrio cholerae GN=flaC PE=3 SV=1
FLAD_VIBCH 100 39880 2 (2) 2 (2) 0.12
Flagellin D OS=Vibrio cholerae GN=flaD PE=1 SV=1
FLAC_VIBC3 100 39881 2 (2) 2 (2) 0.12
Flagellin C OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaC PE=3 SV=1
FLAD_VIBC3 100 39880 2 (2) 2 (2) 0.12
Flagellin D OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaD PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1399   580.2899 1158.5652 1158.5629 2.03 0 65 0.00025 +1Score > 41 indicates identity U K.DDAAGLQISNR.L
2522   558.2933 1671.8581 1671.8540 2.45 1 73 7.5e-006 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.INSAKDDAAGLQISNR.L
2904   944.5114 1887.0082 1887.0200 -6.24 1 12 1.2 +2Score > 38 indicates identity
Score > 25 indicates homology		 U K.QQILQQVSTSILAQAKK.Q + 4 Deamidated (NQ)

1 subset or intersection (4 subset proteins in total)

Score Mass Subset of
FLAB_VIBAN 65 39509 56.1
Flagellin B OS=Vibrio anguillarum GN=flaB PE=1 SV=3
3 samesets of FLAB_VIBAN
FLAB_VIBCH 65 39492
Flagellin B OS=Vibrio cholerae GN=flaB PE=3 SV=1
FLAB_VIBC3 65 39492
Flagellin B OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaB PE=3 SV=1
FLAC_VIBAN 65 40076
Flagellin C OS=Vibrio anguillarum GN=flaC PE=1 SV=3

+57

Accession Score Description
1 ASPQ_PSEPK 100 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+58

Accession Score Description
1 SYA_PSEPG 98 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+59

Accession Score Description
Family member distances as a dendrogram 1 EFTS_PSEPG 96 Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1
2 CLPX_AZOSB 84 ATP-dependent Clp protease ATP-binding subunit clpX OS=Azoarcus sp. (strain BH72) GN=clpX PE=3 SV=1

+60

Accession Score Description
1 CH10_PSEP1 96 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1
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