MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 107 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 51.1 | CLPB_PSEPK |
107 | 94917 | 11 (4) | 9 (4) | 0.10 | |
| Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 | |||||||
11 peptide matches (10 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 155 |
544.3492 | 543.3419 | 543.3380 | 7.14 | 0 | 27 | 2.5 | 5Score > 44 indicates identity |
R.QGLVK.E | ||
| 686 |
422.2570 | 842.4994 | 842.4974 | 2.42 | 0 | 34 | 0.34 | 1Score > 42 indicates identity Score > 41 indicates homology |
R.TVQVLQR.R | ||
| 1120 |
509.8040 | 1017.5934 | 1017.5859 | 7.44 | 0 | 15 | 0.22 | 1Score > 36 indicates identity Score > 21 indicates homology |
U | K.FLPGTAITAK.V | |
| 1139 |
515.2904 | 1028.5662 | 1028.5614 | 4.68 | 1 | 63 | 0.00036 | 1Score > 41 indicates identity |
U | K.TAIAEGLAQR.I | |
| 1387 |
577.8266 | 1153.6386 | 1153.6343 | 3.79 | 1 | 43 | 0.0053 | 1Score > 38 indicates identity Score > 33 indicates homology |
U | R.IINGEVPDGLK.G | |
| 1439 |
587.3124 | 1172.6102 | 1172.6037 | 5.60 | 1 | 29 | 0.11 | 1Score > 41 indicates identity Score > 32 indicates homology |
K.AIDLIDEAASR.I | ||
| 1835 |
678.9076 | 1355.8006 | 1355.7846 | 11.8 | 0 | 50 | 0.00086 | 1Score > 34 indicates identity Score > 31 indicates homology |
U | R.LLALDMGALIAGAK.Y | |
| 2264 |
1 |
783.4681 | 1564.9216 | 1564.8977 | 15.3 | 1 | 45 | 0.00016 | 1Score > 31 indicates identity Score > 20 indicates homology |
U | R.WIENPLAQLILAGK.F |
| 2318 |
529.2911 | 1584.8515 | 1584.8471 | 2.74 | 1 | 6 | 1.2 | 3Score > 39 indicates identity Score > 19 indicates homology |
U | R.VIGQSEAVTAVANAVR.R + Deamidated (NQ) | |
| 2319 |
793.4339 | 1584.8532 | 1584.8471 | 3.87 | 1 | 29 | 0.057 | 1Score > 39 indicates identity Score > 29 indicates homology |
U | R.VIGQSEAVTAVANAVR.R + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| CLPB_PSEAE |
81 | 95061 | 51.1 | ||
| Chaperone protein clpB OS=Pseudomonas aeruginosa GN=clpB PE=3 SV=1 | |||||
| CLPB1_SYNE7 |
77 | 98723 | 51.1 | ||
| Chaperone protein clpB 1 OS=Synechococcus elongatus (strain PCC 7942) GN=clpB1 PE=2 SV=3 | |||||
| 1 sameset of CLPB1_SYNE7 | |||||
| CLPB_PLEBO |
77 | 99301 | |||
| Chaperone protein clpB OS=Plectonema boryanum GN=clpB PE=2 SV=2 | |||||
| CLPAA_SOLLC |
63 | 102892 | 51.1 | ||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4A, chloroplastic OS=Solanum lycopersicum GN=CD4A PE=3 SV=1 | |||||
| 17 samesets of CLPAA_SOLLC | |||||
| CLPB_PSESM |
63 | 95243 | |||
| Chaperone protein clpB OS=Pseudomonas syringae pv. tomato GN=clpB PE=3 SV=1 | |||||
| CLPC_GUITH |
63 | 90960 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Guillardia theta GN=clpC PE=3 SV=1 | |||||
| CLPB2_SYNE7 |
63 | 99931 | |||
| Chaperone protein clpB 2 OS=Synechococcus elongatus (strain PCC 7942) GN=clpB2 PE=3 SV=1 | |||||
| CLPAB_SOLLC |
63 | 102463 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic OS=Solanum lycopersicum GN=CD4B PE=3 SV=1 | |||||
| CLPA_BRANA |
63 | 97430 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog, chloroplastic (Fragment) OS=Brassica napus GN=CLPA PE=2 SV=1 | |||||
| CLPB_GLOVI |
63 | 98196 | |||
| Chaperone protein clpB OS=Gloeobacter violaceus GN=clpB PE=3 SV=1 | |||||
| CLPC_CYACA |
63 | 95688 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Cyanidium caldarium GN=clpC PE=3 SV=1 | |||||
| CLPC_PEA |
63 | 102818 | |||
| ATP-dependent Clp protease ATP-binding subunit clpC homolog, chloroplastic OS=Pisum sativum PE=2 SV=1 | |||||
| CLPC_PORPU |
63 | 91062 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Porphyra purpurea GN=clpC PE=3 SV=1 | |||||
| CLPC_PORYE |
63 | 91042 | |||
| ATP-dependent Clp protease ATP-binding subunit clpA homolog OS=Porphyra yezoensis GN=clpC PE=3 SV=1 | |||||
| CLP_TRYBB |
63 | 97187 | |||
| Heat shock protein 100 OS=Trypanosoma brucei brucei GN=HSP100 PE=3 SV=1 | |||||
| CLPB1_SYNY3 |
63 | 101443 | |||
| Chaperone protein clpB 1 OS=Synechocystis sp. (strain PCC 6803) GN=clpB1 PE=3 SV=1 | |||||
| CLPB1_THEEB |
63 | 98443 | |||
| Chaperone protein clpB 1 OS=Thermosynechococcus elongatus (strain BP-1) GN=clpB1 PE=3 SV=1 | |||||
| CLPB2_ANASP |
63 | 98620 | |||
| Chaperone protein clpB 2 OS=Anabaena sp. (strain PCC 7120) GN=clpB2 PE=3 SV=2 | |||||
| CLPB2_SYNY3 |
63 | 98119 | |||
| Chaperone protein clpB 2 OS=Synechocystis sp. (strain PCC 6803) GN=clpB2 PE=3 SV=1 | |||||
| CLPB2_THEEB |
63 | 99950 | |||
| Chaperone protein clpB 2 OS=Thermosynechococcus elongatus (strain BP-1) GN=clpB2 PE=3 SV=1 | |||||
| CLPB_PARUW |
63 | 98713 | |||
| Chaperone protein clpB OS=Protochlamydia amoebophila (strain UWE25) GN=clpB PE=3 SV=1 | |||||
| CLPB_COXBU |
50 | 96823 | 51.1 | ||
| Chaperone protein clpB OS=Coxiella burnetii GN=clpB PE=3 SV=1 | |||||
| 1 sameset of CLPB_COXBU | |||||
| CLPB_RHOPA |
50 | 96669 | |||
| Chaperone protein clpB OS=Rhodopseudomonas palustris GN=clpB PE=3 SV=1 | |||||
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSB_ECOLI | 103 | Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO1_AZOVI | 101 | 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_AZOVD | 101 | 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 100 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FLAE_VIBAN | 100 | Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ASPQ_PSEPK | 100 | Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 98 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTS_PSEPG | 96 | Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1 | |
| 2 | CLPX_AZOSB | 84 | ATP-dependent Clp protease ATP-binding subunit clpX OS=Azoarcus sp. (strain BH72) GN=clpX PE=3 SV=1 | |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH10_PSEP1 | 96 | 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1 |
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