MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 240)

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+41

Accession Score Description
1 HYDA_PSEPU 128 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2

+42

Accession Score Description
1 RS2_PSEP1 125 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+43

Accession Score Description
1 RS16_PSEP1 121 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+44

Accession Score Description
1 RL25_PSEPK 117 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+45

Accession Score Description
1 IDH_AZOVI 117 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+46

Accession Score Description
1 GLPK_PSEPG 115 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+47

Accession Score Description
1 ILVC_PSEPG 113 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+48

Accession Score Description
1 RL11_PSEE4 112 50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1

+49

Accession Score Description
1 TIG_PSEPG 112 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

-50

Accession Score Description
1 SUCD_PSEAE 111 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2
Score Mass Matches Sequences emPAI
50.1 SUCD_PSEAE 111 30646 9 (6) 6 (5) 0.55
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

-9 peptide matches (8 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
401   703.4023 702.3950 702.3912 5.46 0 50 0.01 +2Score > 43 indicates identity U R.SLADIGK.A
402   352.2049 702.3952 702.3912 5.78 0 23 4.1 +6Score > 43 indicates identity
Score > 42 indicates homology		 U R.SLADIGK.A
1121   510.2817 1018.5488 1018.5447 4.03 0 36 0.058 +1Score > 41 indicates identity
Score > 36 indicates homology		 U K.FAALQDAGVK.T
1211   534.7857 1067.5568 1067.5499 6.53 1 73 8e-006 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.SGTLTYEAVK.Q
1543   610.8373 1219.6600 1219.6448 12.5 1 39 0.0071 +1Score > 40 indicates identity
Score > 30 indicates homology		 U K.DSILEAAFGGIK.L
1857   343.2016 1368.7773 1368.7700 5.33 0 32 0.01 +1Score > 37 indicates identity
Score > 25 indicates homology		 U K.IGIMPGHIHLPGK.V
2208 +1 514.2928 1539.8566 1539.8410 10.1 0 27 0.02 +1Score > 37 indicates identity
Score > 22 indicates homology		 U K.GGTTHLGLPVFNTVK.E
2209   770.9365 1539.8584 1539.8410 11.4 0 40 0.002 +1Score > 37 indicates identity
Score > 25 indicates homology		 U K.GGTTHLGLPVFNTVK.E

2 subsets and intersections (24 subset proteins in total)

Score Mass Subset of
SUCD_ECO57 73 30044 50.1
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O157:H7 GN=sucD PE=3 SV=2
3 samesets of SUCD_ECO57
SUCD_ECOLI 73 30044
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli (strain K12) GN=sucD PE=1 SV=2
SUCD_HAEIN 73 30769
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Haemophilus influenzae GN=sucD PE=3 SV=1
SUCD_ECOL6 73 30044
Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Escherichia coli O6 GN=sucD PE=3 SV=2
DNAA_RICAE 50 0 50.1
description
19 samesets of DNAA_RICAE
S4A4_AMBTI 50 116888
Electrogenic sodium bicarbonate cotransporter 1 OS=Ambystoma tigrinum GN=SLC4A4 PE=2 SV=1
S4A4_BOVIN 50 122108
Electrogenic sodium bicarbonate cotransporter 1 OS=Bos taurus GN=SLC4A4 PE=1 SV=1
S4A4_HUMAN 50 122295
Electrogenic sodium bicarbonate cotransporter 1 OS=Homo sapiens GN=SLC4A4 PE=1 SV=1
S4A4_MOUSE 50 122318
Electrogenic sodium bicarbonate cotransporter 1 OS=Mus musculus GN=Slc4a4 PE=1 SV=2
S4A4_PIG 50 122187
Electrogenic sodium bicarbonate cotransporter 1 OS=Sus scrofa GN=SLC4A4 PE=1 SV=1
S4A4_RABIT 50 122204
Electrogenic sodium bicarbonate cotransporter 1 OS=Oryctolagus cuniculus GN=SLC4A4 PE=1 SV=1
S4A4_RAT 50 122178
Electrogenic sodium bicarbonate cotransporter 1 OS=Rattus norvegicus GN=Slc4a4 PE=1 SV=1
S4A5_HUMAN 50 127258
Electrogenic sodium bicarbonate cotransporter 4 OS=Homo sapiens GN=SLC4A5 PE=2 SV=2
S4A5_RAT 50 124721
Electrogenic sodium bicarbonate cotransporter 4 OS=Rattus norvegicus GN=Slc4a5 PE=1 SV=1
DNAA_RICAH 50 0
description
DNAA_RICCK 50 0
description
DNAA_RICCN 50 0
description
DNAA_RICFE 50 0
description
DNAA_RICPR 50 0
description
DNAA_RICPU 50 0
description
DNAA_RICRO 50 0
description
DNAA_RICRS 50 0
description
DNAA_RICTY 50 0
description
DNAA_RICM5 50 0
description
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