MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 240)


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+41

Accession Score Description
1 HYDA_PSEPU 128 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2

+42

Accession Score Description
1 RS2_PSEP1 125 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+43

Accession Score Description
1 RS16_PSEP1 121 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+44

Accession Score Description
1 RL25_PSEPK 117 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+45

Accession Score Description
1 IDH_AZOVI 117 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+46

Accession Score Description
1 GLPK_PSEPG 115 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+47

Accession Score Description
1 ILVC_PSEPG 113 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

-48

Accession Score Description
1 RL11_PSEE4 112 50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1
Score Mass Matches Sequences emPAI
48.1 RL11_PSEE4 112 14940 7 (6) 5 (5) 1.41
50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1
4 samesets of RL11_PSEE4
RL11_PSEP1 112 14914 7 (6) 5 (5) 1.43
50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
RL11_PSEPG 112 14914 7 (6) 5 (5) 1.43
50S ribosomal protein L11 OS=Pseudomonas putida (strain GB-1) GN=rplK PE=3 SV=1
RL11_PSEPK 112 14914 7 (6) 5 (5) 1.43
50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1
RL11_PSEPW 112 14914 7 (6) 5 (5) 1.43
50S ribosomal protein L11 OS=Pseudomonas putida (strain W619) GN=rplK PE=3 SV=1

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
727   430.2149 858.4152 858.4123 3.39 1 30 0.059 +1Score > 40 indicates identity
Score > 30 indicates homology
U R.SFTFETK.S
790   444.2473 886.4800 886.4760 4.60 1 46 0.025 +1Score > 42 indicates identity U R.AQLEDIAK.A
860   915.5551 914.5478 914.5437 4.54 0 46 0.012 +1Score > 39 indicates identity U K.STPASVLLK.K
861   458.2829 914.5512 914.5437 8.29 0 25 0.2 +1Score > 38 indicates identity
Score > 30 indicates homology
U K.STPASVLLK.K
1985   715.3714 1428.7282 1428.7209 5.17 0 86 4.1e-007 +1Score > 41 indicates identity
Score > 34 indicates homology
U K.QADLTAADLDAAVR.T
1987   477.2656 1428.7750 1428.7685 4.55 0 32 0.0066 +1Score > 40 indicates identity
Score > 23 indicates homology
U K.AAGLTSGSARPNTVK.V
1988   715.3955 1428.7764 1428.7685 5.58 0 44 0.014 +1Score > 40 indicates identity
Score > 38 indicates homology
U K.AAGLTSGSARPNTVK.V

10 subsets and intersections (56 subset proteins in total)

Score Mass Subset of
RL11_PSEMY 107 14959 48.1
50S ribosomal protein L11 OS=Pseudomonas mendocina (strain ymp) GN=rplK PE=3 SV=1
RL11_CHRVO 86 15002 48.1
50S ribosomal protein L11 OS=Chromobacterium violaceum GN=rplK PE=3 SV=1
RL11_PSEA7 84 14955 48.1
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain PA7) GN=rplK PE=3 SV=1
3 samesets of RL11_PSEA7
RL11_PSEA8 84 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplK PE=3 SV=1
RL11_PSEAB 84 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplK PE=3 SV=1
RL11_PSEAE 84 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa GN=rplK PE=3 SV=1
RL11_PSESM 60 14930 48.1
50S ribosomal protein L11 OS=Pseudomonas syringae pv. tomato GN=rplK PE=3 SV=1
1 sameset of RL11_PSESM
RL11_PSEU2 60 14930
50S ribosomal protein L11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplK PE=3 SV=1
RL11_PSEF5 55 14960 48.1
50S ribosomal protein L11 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplK PE=3 SV=1
2 samesets of RL11_PSEF5
RL11_PSEFS 55 14960
50S ribosomal protein L11 OS=Pseudomonas fluorescens (strain SBW25) GN=rplK PE=3 SV=1
RL11_PSEPF 55 14934
50S ribosomal protein L11 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplK PE=3 SV=1
RL11_PSE14 48 14911 48.1
50S ribosomal protein L11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplK PE=3 SV=1
RL11_XANAC 46 14888 48.1
50S ribosomal protein L11 OS=Xanthomonas axonopodis pv. citri GN=rplK PE=3 SV=1
7 samesets of RL11_XANAC
RL11_XANC5 46 14902
50S ribosomal protein L11 OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=rplK PE=3 SV=1
RL11_XANC8 46 14880
50S ribosomal protein L11 OS=Xanthomonas campestris pv. campestris (strain 8004) GN=rplK PE=3 SV=1
RL11_XANCB 46 14852
50S ribosomal protein L11 OS=Xanthomonas campestris pv. campestris (strain B100) GN=rplK PE=3 SV=1
RL11_XANCP 46 14880
50S ribosomal protein L11 OS=Xanthomonas campestris pv. campestris GN=rplK PE=3 SV=1
RL11_XANOM 46 15062
50S ribosomal protein L11 OS=Xanthomonas oryzae pv. oryzae (strain MAFF 311018) GN=rplK PE=3 SV=1
RL11_XANOP 46 15062
50S ribosomal protein L11 OS=Xanthomonas oryzae pv. oryzae (strain PXO99A) GN=rplK PE=3 SV=1
RL11_XANOR 46 15062
50S ribosomal protein L11 OS=Xanthomonas oryzae pv. oryzae GN=rplK PE=3 SV=1
RL11_ACIF2 46 15022 48.1
50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=rplK PE=3 SV=1
2 samesets of RL11_ACIF2
RL11_ACIF5 46 15022
50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=rplK PE=3 SV=1
RL11_PRB01 46 14968
50S ribosomal protein L11 OS=Gamma-proteobacterium EBAC31A08 GN=rplK PE=3 SV=1
RL11_AZOVD 42 15015 48.1
50S ribosomal protein L11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplK PE=3 SV=1
RL11_AERHH 30 15094 48.1
50S ribosomal protein L11 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplK PE=3 SV=1
+31 samesets of RL11_AERHH

+49

Accession Score Description
1 TIG_PSEPG 112 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+50

Accession Score Description
1 SUCD_PSEAE 111 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2
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