Rita5 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita5 sp
MS data file	:	PRT1270_T-BRSC_5_20250714121805.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:42:02 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita5 sp

MS data file

PRT1270_T-BRSC_5_20250714121805.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:42:02 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,997

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,997

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

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Protein Family Summary

Protein families 41–50 (out of 240)

Previous 1 2 3 4 5 6 7 8 9 10 … 24 Next

41

		Accession	Score	Description
	1	HYDA_PSEPU	128	D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2

42

		Accession	Score	Description
	1	RS2_PSEP1	125	30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

43

		Accession	Score	Description
	1	RS16_PSEP1	121	30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

44

		Accession	Score	Description
	1	RL25_PSEPK	117	50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

45

		Accession	Score	Description
	1	IDH_AZOVI	117	Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

46

		Accession	Score	Description
	1	GLPK_PSEPG	115	Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

47

		Accession	Score	Description
	1	ILVC_PSEPG	113	Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
47.1	ILVC_PSEPG	113	36575	4 (4)	4 (4)	0.28
	Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1
	3 samesets of ILVC_PSEPG
	ILVC_PSEPK	113	36575	4 (4)	4 (4)	0.28
	Ketol-acid reductoisomerase OS=Pseudomonas putida (strain KT2440) GN=ilvC PE=3 SV=1
	ILVC_PSEPW	113	36561	4 (4)	4 (4)	0.28
	Ketol-acid reductoisomerase OS=Pseudomonas putida (strain W619) GN=ilvC PE=3 SV=1
	ILVC_PSEP1	113	36575	4 (4)	4 (4)	0.28
	Ketol-acid reductoisomerase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ilvC PE=3 SV=1

4 peptide matches (4 non-duplicate, 0 duplicate)

Query	Observed	Mr(expt)	Mr(calc)	ppm	Score	Expect	Rank	U	Peptide
1466	593.3422	1184.6698	1184.6475	18.9	45	0.0053	1Score > 39 indicates identity Score > 35 indicates homology	U	R.ADLDVIMIAPK.A
1730	654.3401	1306.6656	1306.6630	2.05	53	0.00012	1Score > 41 indicates identity Score > 26 indicates homology	U	K.NVALSYASGVGGGR.T
2848	930.4897	1858.9648	1858.9425	12.0	59	5.1e-005	1Score > 39 indicates identity Score > 29 indicates homology	U	K.GGGIPDLIAIYQDASGNAK.N
3465	551.2900	2201.1309	2201.1018	13.2	36	0.00065	1Score > 38 indicates identity Score > 17 indicates homology	U	K.GATLAFSHGFSIHYNQVVPR.A + Deamidated (NQ)

5 subsets and intersections (55 subset proteins in total)

	Score	Mass	Subset of
ILVC_PSEE4	97	36674	47.1
Ketol-acid reductoisomerase OS=Pseudomonas entomophila (strain L48) GN=ilvC PE=3 SV=1
ILVC_PSEA7	72	36729	47.1
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain PA7) GN=ilvC PE=3 SV=1
6 samesets of ILVC_PSEA7
ILVC_PSEAB	72	36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=ilvC PE=3 SV=1
ILVC_PSEAE	72	36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa GN=ilvC PE=1 SV=1
ILVC_PSEFS	72	36416
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvC PE=3 SV=1
ILVC_PSEPF	72	36441
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ilvC PE=3 SV=1
ILVC_PSEF5	72	36441
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=ilvC PE=3 SV=1
ILVC_PSEA8	72	36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain LESB58) GN=ilvC PE=3 SV=1
ILVC_PSE14	67	36531	47.1
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=ilvC PE=3 SV=1
3 samesets of ILVC_PSE14
ILVC_PSEMY	67	36501
Ketol-acid reductoisomerase OS=Pseudomonas mendocina (strain ymp) GN=ilvC PE=3 SV=1
ILVC_PSESM	67	36589
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. tomato GN=ilvC PE=3 SV=1
ILVC_PSEU2	67	36531
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ilvC PE=3 SV=1
ILVC_ACIAD	53	37091	47.1
Ketol-acid reductoisomerase OS=Acinetobacter sp. (strain ADP1) GN=ilvC PE=3 SV=1
6 samesets of ILVC_ACIAD
ILVC_ACIBC	53	37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain ACICU) GN=ilvC PE=3 SV=1
ILVC_ACIBS	53	37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain SDF) GN=ilvC PE=3 SV=1
ILVC_ACIBT	53	37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=ilvC PE=3 SV=2
ILVC_ACIBY	53	37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AYE) GN=ilvC PE=3 SV=1
ILVC_ACIB3	53	37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AB307-0294) GN=ilvC PE=3 SV=1
ILVC_ACIB5	53	37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AB0057) GN=ilvC PE=3 SV=1
ILVC_ALCBS	45	36559	47.1
Ketol-acid reductoisomerase OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=ilvC PE=3 SV=2
35 samesets of ILVC_ALCBS

48

		Accession	Score	Description
	1	RL11_PSEE4	112	50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1

49

		Accession	Score	Description
	1	TIG_PSEPG	112	Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

50

		Accession	Score	Description
	1	SUCD_PSEAE	111	Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

Previous 1 2 3 4 5 6 7 8 9 10 … 24 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 41–50 (out of 240)

41

42

43

44

45

46

47

4 peptide matches (4 non-duplicate, 0 duplicate)

5 subsets and intersections (55 subset proteins in total)

48

49

50