MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HYDA_PSEPU | 128 | D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 125 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS16_PSEP1 | 121 | 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 117 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 44.1 | RL25_PSEPK |
117 | 21183 | 6 (4) | 6 (4) | 0.52 | |
| 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 | |||||||
6 peptide matches (6 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 355 |
343.7146 | 685.4146 | 685.4123 | 3.46 | 0 | 8 | 1.7 | 5Score > 42 indicates identity Score > 23 indicates homology |
U | K.QNVVVK.A | |
| 911 |
312.1792 | 933.5158 | 933.5144 | 1.43 | 0 | 43 | 0.00053 | 1Score > 40 indicates identity Score > 22 indicates homology |
U | K.AVANVHAPR.V | |
| 1382 |
575.7986 | 1149.5826 | 1149.5778 | 4.20 | 1 | 50 | 0.00051 | 1Score > 42 indicates identity Score > 29 indicates homology |
U | M.TEFTLNAQAR.T | |
| 1481 |
398.2050 | 1191.5932 | 1191.5859 | 6.10 | 0 | 25 | 0.074 | 1Score > 42 indicates identity Score > 26 indicates homology |
U | K.GFIMHADFVR.V | |
| 1837 |
453.2317 | 1356.6733 | 1356.6674 | 4.34 | 1 | 39 | 0.011 | 1Score > 40 indicates identity Score > 32 indicates homology |
U | K.GVEFVALAHGDDK.A | |
| 3856 |
823.4471 | 2467.3195 | 2467.2707 | 19.8 | 0 | 74 | 8.5e-006 | 1Score > 35 indicates identity |
U | R.HSANIPAVVYGGNQDAQSLTILAK.E + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL25_PSEE4 |
55 | 21144 | 44.1 | ||
| 50S ribosomal protein L25 OS=Pseudomonas entomophila (strain L48) GN=rplY PE=3 SV=1 | |||||
| 4 samesets of RL25_PSEE4 | |||||
| RL25_PSEPG |
55 | 21042 | |||
| 50S ribosomal protein L25 OS=Pseudomonas putida (strain GB-1) GN=rplY PE=3 SV=1 | |||||
| RL25_PSEPW |
55 | 21299 | |||
| 50S ribosomal protein L25 OS=Pseudomonas putida (strain W619) GN=rplY PE=3 SV=1 | |||||
| RL25_PSEP1 |
55 | 21342 | |||
| 50S ribosomal protein L25 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplY PE=3 SV=1 | |||||
| RL25_PSEPF |
55 | 21321 | |||
| 50S ribosomal protein L25 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplY PE=3 SV=1 | |||||
| RL25_PSEFS |
43 | 21559 | 44.1 | ||
| 50S ribosomal protein L25 OS=Pseudomonas fluorescens (strain SBW25) GN=rplY PE=3 SV=1 | |||||
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IDH_AZOVI | 117 | Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPK_PSEPG | 115 | Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 113 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEE4 | 112 | 50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPG | 112 | Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 111 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
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