MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 240)

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+41

Accession Score Description
1 HYDA_PSEPU 128 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2

-42

Accession Score Description
1 RS2_PSEP1 125 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
Score Mass Matches Sequences emPAI
42.1 RS2_PSEP1 125 27063 15 (7) 9 (6) 0.64
30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
2 samesets of RS2_PSEP1
RS2_PSEPK 125 27063 15 (7) 9 (6) 0.64
30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1
RS2_PSEPW 125 27095 10 (7) 7 (6) 0.64
30S ribosomal protein S2 OS=Pseudomonas putida (strain W619) GN=rpsB PE=3 SV=1

-15 peptide matches (13 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
441   363.7018 725.3890 725.3860 4.14 0 14 0.27 +1Score > 37 indicates identity
Score > 21 indicates homology		 K.YIFGAR.N
442   726.3969 725.3896 725.3860 4.93 0 18 0.38 +1Score > 36 indicates identity
Score > 27 indicates homology		 K.YIFGAR.N
551 +2 389.2491 776.4836 776.4796 5.17 0 28 0.17 +1Score > 33 indicates identity K.ILFVGTK.R
719   857.4863 856.4790 856.4766 2.78 1 41 0.046 +1Score > 40 indicates identity U K.IVAEQAAR.C
720   429.2480 856.4814 856.4766 5.61 1 38 0.091 +1Score > 40 indicates identity U K.IVAEQAAR.C
729   859.4907 858.4834 858.4810 2.77 1 52 0.006 +1Score > 43 indicates identity U R.IAITEANK.L
995   322.5319 964.5739 964.5705 3.45 1 21 0.88 +1Score > 34 indicates identity
Score > 33 indicates homology		 U K.IHIVNLEK.T
996   483.2949 964.5752 964.5705 4.88 1 12 0.68 +1Score > 34 indicates identity
Score > 23 indicates homology		 U K.IHIVNLEK.T
1284   370.5264 1108.5574 1108.5526 4.27 0 29 0.035 +1Score > 41 indicates identity
Score > 27 indicates homology		 U K.AGVHFGHQTR.Y
1459   591.8083 1181.6020 1181.5903 9.94 0 30 0.0075 +1Score > 40 indicates identity
Score > 21 indicates homology		 U R.WLGGMLTNYK.T
2450   820.4212 1638.8278 1638.8076 12.4 1 58 4.9e-005 +1Score > 40 indicates identity
Score > 27 indicates homology		 U K.TLPMFNDALSFVER.L
4433   1032.8811 3095.6215 3095.5663 17.8 1 53 0.00067 +1Score > 34 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4434   1032.8818 3095.6236 3095.5663 18.5 1 55 0.00044 +1Score > 34 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)

+14 subsets and intersections (335 subset proteins in total)

+43

Accession Score Description
1 RS16_PSEP1 121 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+44

Accession Score Description
1 RL25_PSEPK 117 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+45

Accession Score Description
1 IDH_AZOVI 117 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+46

Accession Score Description
1 GLPK_PSEPG 115 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+47

Accession Score Description
1 ILVC_PSEPG 113 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+48

Accession Score Description
1 RL11_PSEE4 112 50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1

+49

Accession Score Description
1 TIG_PSEPG 112 Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

+50

Accession Score Description
1 SUCD_PSEAE 111 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2
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