MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 240)

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+31

Accession Score Description
1 STHA_PSEPG 155 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+32

Accession Score Description
1 GUAA_CELJU 155 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+33

Accession Score Description
1 PNP_PSEPK 150 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1

+34

Accession Score Description
1 AMPA_PSEPG 149 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+35

Accession Score Description
1 ATPG_PSEPG 148 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+36

Accession Score Description
1 ODO2_PSEAE 136 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+37

Accession Score Description
1 RL5_PSEE4 134 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+38

Accession Score Description
1 CISY_PSEAE 132 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+39

Accession Score Description
1 RL21_PSEE4 130 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

-40

Accession Score Description
1 RL2_PSEP1 129 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
Score Mass Matches Sequences emPAI
40.1 RL2_PSEP1 129 29822 12 (5) 10 (5) 0.45
50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
4 samesets of RL2_PSEP1
RL2_PSEPK 129 29822 12 (5) 10 (5) 0.45
50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1
RL2_PSEPW 129 29822 12 (5) 10 (5) 0.45
50S ribosomal protein L2 OS=Pseudomonas putida (strain W619) GN=rplB PE=3 SV=1
RL2_PSEE4 129 29808 10 (5) 9 (5) 0.45
50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1
RL2_PSEPG 129 29794 9 (5) 8 (5) 0.45
50S ribosomal protein L2 OS=Pseudomonas putida (strain GB-1) GN=rplB PE=3 SV=1

-12 peptide matches (11 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
287   325.1882 648.3618 648.3595 3.60 0 20 0.1 +1Score > 40 indicates identity
Score > 22 indicates homology		 R.LVDFR.R
407   352.7220 703.4294 703.4268 3.72 0 21 0.86 +1Score > 35 indicates identity
Score > 33 indicates homology		 R.YIIAPK.G
562   392.7447 783.4748 783.4715 4.24 0 30 0.067 +1Score > 36 indicates identity
Score > 30 indicates homology		 R.GVRPTVR.G
723   429.7442 857.4738 857.4719 2.28 0 48 0.0087 +1Score > 43 indicates identity
Score > 40 indicates homology		 U K.AGNSLQLR.N
916   468.7621 935.5096 935.5076 2.16 1 11 0.96 +1Score > 40 indicates identity
Score > 23 indicates homology		 U R.EGVYVTLR.L
1149 +1 344.8672 1031.5798 1031.5764 3.31 1 30 0.022 +1Score > 40 indicates identity
Score > 26 indicates homology		 U K.GAPHAPLIEK.K
1174   522.7975 1043.5804 1043.5723 7.78 0 58 0.00039 +1Score > 41 indicates identity
Score > 36 indicates homology		 U R.SAGASAQLIAR.E
1628   626.8306 1251.6466 1251.6401 5.26 0 42 0.0045 +1Score > 40 indicates identity
Score > 31 indicates homology		 U R.HPVSPWGFPTK.G
2303   790.9623 1579.9100 1579.8933 10.6 0 85 4.1e-007 +1Score > 34 indicates identity U K.GVSAGDQLIAGALAPIK.A
2669   587.3413 1759.0021 1758.9992 1.63 1 6 0.49 +1Score > 33 indicates identity
Score > 15 indicates homology		 U R.NIPVGSTIHGIELKPGK.G
2670   440.7600 1759.0109 1758.9992 6.65 1 22 0.59 +1Score > 32 indicates identity U R.NIPVGSTIHGIELKPGK.G

5 subsets and intersections (16 subset proteins in total)

Score Mass Subset of
RL2_PSE14 68 29789 40.1
50S ribosomal protein L2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplB PE=3 SV=1
4 samesets of RL2_PSE14
RL2_PSEFS 68 29803
50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain SBW25) GN=rplB PE=3 SV=1
RL2_PSEPF 68 29773
50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplB PE=3 SV=1
RL2_PSESM 68 29833
50S ribosomal protein L2 OS=Pseudomonas syringae pv. tomato GN=rplB PE=3 SV=1
RL2_PSEU2 68 29789
50S ribosomal protein L2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplB PE=3 SV=1
RL2_PSEMY 67 29847 40.1
50S ribosomal protein L2 OS=Pseudomonas mendocina (strain ymp) GN=rplB PE=3 SV=1
RL2_PSEF5 43 29745 40.1
50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplB PE=3 SV=1
RL2_AZOVD 42 29689 40.1
50S ribosomal protein L2 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplB PE=3 SV=1
2 samesets of RL2_AZOVD
RL2_PSEU5 42 29761
50S ribosomal protein L2 OS=Pseudomonas stutzeri (strain A1501) GN=rplB PE=3 SV=1
RL2_NEOSM 42 30311
50S ribosomal protein L2 OS=Neorickettsia sennetsu (strain Miyayama) GN=rplB PE=3 SV=1
RL2_RALEH 30 30215 40.1
50S ribosomal protein L2 OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=rplB PE=3 SV=1
5 samesets of RL2_RALEH
RL2_SACD2 30 30282
50S ribosomal protein L2 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplB PE=3 SV=1
RL2_RALPJ 30 30251
50S ribosomal protein L2 OS=Ralstonia pickettii (strain 12J) GN=rplB PE=3 SV=1
RL2_RALSO 30 30198
50S ribosomal protein L2 OS=Ralstonia solanacearum GN=rplB PE=3 SV=1
RL2_RALEJ 30 30053
50S ribosomal protein L2 OS=Ralstonia eutropha (strain JMP134) GN=rplB PE=3 SV=1
RL2_RALME 30 30115
50S ribosomal protein L2 OS=Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) GN=rplB PE=3 SV=1
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