MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)

Page: 1 2 3 4 5 6 … 24 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1

-4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
ATPA_PSEPK 777 55489 51 (32) 24 (14) 1.15
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
1 sameset of ATPA_PSEPK
ATPA_PSEP1 777 55458 51 (32) 24 (14) 1.15
ATP synthase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpA PE=3 SV=1
ATPA_RICAH 168 56389 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
7 samesets of ATPA_RICAH
ATPA_RICCN 168 56128 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia conorii GN=atpA PE=3 SV=2
ATPA_RICFE 168 56172 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia felis GN=atpA PE=3 SV=1
ATPA_RICPU 168 56160 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia peacockii (strain Rustic) GN=atpA PE=3 SV=1
ATPA_RICRO 168 56195 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Iowa) GN=atpA PE=3 SV=2
ATPA_RICRS 168 56195 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Sheila Smith) GN=atpA PE=3 SV=1
ATPA_RICM5 168 56127 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia massiliae (strain Mtu5) GN=atpA PE=3 SV=2
ATPA_RICB8 168 56616 14 (11) 5 (4) 0.27
ATP synthase subunit alpha OS=Rickettsia bellii (strain OSU 85-389) GN=atpA PE=3 SV=1
ATPA_VEREI 155 57757 10 (5) 4 (2) 0.12
ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-56 peptide matches (37 non-duplicate, 19 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
201 +1 301.6858 601.3570 601.3548 3.82 0 32 0.18 +1Score > 43 indicates identity
Score > 37 indicates homology		 X K.LSGGIR.T
202   602.3646 601.3573 601.3548 4.28 0 16 1.6 +9Score > 43 indicates identity
Score > 31 indicates homology		 X K.LSGGIR.T
212   606.2911 605.2838 605.2809 4.78 0 12 0.25 +1Score > 41 indicates identity
Score > 18 indicates homology		 X K.ATQTW.-
263 +1 318.1810 634.3474 634.3438 5.68 0 36 0.033 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X E.LAQFR.E + Deamidated (NQ)
410   707.3874 706.3801 706.3762 5.55 0 29 0.43 +1Score > 39 indicates identity
Score > 38 indicates homology		 X X K.QAVAYR.Q
434   360.7029 719.3912 719.3887 3.48 1 29 0.14 +1Score > 41 indicates identity
Score > 33 indicates homology		 X R.VTELMK.Q
630   408.2345 814.4544 814.4548 -0.49 1 17 1.2 +10Score > 43 indicates identity
Score > 31 indicates homology		 X X X R.ELIIGDR.Q
631   815.4648 814.4575 814.4548 3.28 1 43 0.055 +1Score > 43 indicates identity X X X R.ELIIGDR.Q
638   411.7281 821.4416 821.4395 2.58 0 32 0.41 +1Score > 40 indicates identity X R.TALAQYR.E
639   822.4493 821.4420 821.4395 3.04 0 31 0.48 +1Score > 40 indicates identity X R.TALAQYR.E
682 +2 421.7792 841.5438 841.5385 6.34 0 43 0.015 +1Score > 37 indicates identity U X X R.QISLLLR.R
683   842.5513 841.5440 841.5385 6.55 0 59 0.00035 +1Score > 37 indicates identity U X X R.QISLLLR.R
709 +2 427.7721 853.5296 853.5273 2.75 1 42 0.0092 +1Score > 34 indicates identity U X R.ILEVPVGK.E
710   854.5381 853.5308 853.5273 4.13 1 28 0.15 +1Score > 33 indicates identity U X R.ILEVPVGK.E
731   859.5034 858.4961 858.4923 4.44 0 35 0.28 +1Score > 42 indicates identity U X R.STVANIVR.K
809   447.7400 893.4654 893.4607 5.32 0 41 0.012 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X K.FTNGAVTGK.T
811   448.2297 894.4448 894.4447 0.16 0 34 0.011 +1Score > 41 indicates identity
Score > 27 indicates homology		 U X K.FTNGAVTGK.T + Deamidated (NQ)
815 +2 449.2688 896.5230 896.5232 -0.20 0 46 0.006 +1Score > 36 indicates identity U X K.VAPGVIWR.K
816   897.5327 896.5254 896.5232 2.45 0 29 0.093 +1Score > 36 indicates identity
Score > 31 indicates homology		 U X K.VAPGVIWR.K
1173   522.7821 1043.5496 1043.5434 6.02 0 14 0.25 +1Score > 41 indicates identity
Score > 20 indicates homology		 U X K.SVDAMIPVGR.G
1279 +1 553.8134 1105.6122 1105.6019 9.33 1 41 0.00099 +1Score > 39 indicates identity
Score > 24 indicates homology		 U X R.GFLIDVEVSK.I
1488 +1 599.3302 1196.6458 1196.6401 4.80 0 51 0.0033 +1Score > 39 indicates identity U X X R.VVDALGNPIDGK.G
1534   609.3104 1216.6062 1216.6048 1.22 0 45 0.027 +1Score > 42 indicates identity U X R.IDNLDVSSQAR.N
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_5_20250714121805.raw

Score > 42 indicates identity

Score > 28 indicates homology
1546   611.3119 1220.6092 1220.6037 4.52 0 21 0.27 -1Score > 42 indicates identity
Score > 28 indicates homology		 U X K.SVDQPVQTGYK.S
4.53 0 21 0.27 1 SVNQPVQTGYK   + Deamidated (NQ)
-16.1 1 14 1.3 3 ADGKILDTFLE  
4.53 0 13 2.1 4 SVNQPVQTGYK   + Deamidated (NQ)
-16.0 1 10 3.9 5 KNINIDDFLK   + 2 Deamidated (NQ)
-16.0 1 10 3.9 5 QDLAYLQELK   + Deamidated (NQ)
4.57 1 7 6.6 7 ASAAPEIYSANK  
19.1 0 7 7.7 8 GADIFLGCSGPK  
1.80 1 7 8.2 9 AGDLKNMSQLK   + Deamidated (NQ); Oxidation (M)
1.80 1 7 8.2 9 AGDLKNMSQLK   + Deamidated (NQ); Oxidation (M)
1689   644.8590 1287.7034 1287.6856 13.8 0 15 0.86 +2Score > 40 indicates identity
Score > 27 indicates homology		 X K.TAMAIDAIINQK.D
1698 +1 647.3049 1292.5952 1292.5885 5.26 1 95 6.5e-008 +1Score > 40 indicates identity
Score > 36 indicates homology		 U X K.GDFNDEIDAGLK.A
1779 +1 665.3383 1328.6620 1328.6572 3.64 1 103 1.2e-008 +1Score > 41 indicates identity
Score > 37 indicates homology		 U X K.GPLGNTQTDAVEK.V
1816   675.3599 1348.7052 1348.6987 4.87 1 86 1.7e-007 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X R.KSVDQPVQTGYK.S
1991   477.5852 1429.7338 1429.7273 4.50 1 41 0.024 +1Score > 41 indicates identity
Score > 38 indicates homology		 U X K.GRIDNLDVSSQAR.N
1992   715.8749 1429.7352 1429.7273 5.53 1 76 5.5e-006 +1Score > 41 indicates identity
Score > 36 indicates homology		 U X K.GRIDNLDVSSQAR.N
1993 +1 716.3802 1430.7458 1430.7365 6.50 1 78 3e-006 +1Score > 41 indicates identity
Score > 35 indicates homology		 U X R.NEGTVVSVSDGIVR.I
2235 +1 777.3773 1552.7400 1552.7310 5.81 1 86 1.6e-006 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2237 +1 518.5879 1552.7419 1552.7310 6.98 1 55 0.0019 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2380 +1 538.2962 1611.8668 1611.8409 16.0 1 74 1.6e-005 +1Score > 39 indicates identity U X K.IGSFEQALIAFFNR.D
2381 +2 806.9411 1611.8676 1611.8409 16.6 1 79 4.3e-007 +1Score > 39 indicates identity
Score > 28 indicates homology		 U X K.IGSFEQALIAFFNR.D
2578   854.9265 1707.8384 1707.8315 4.05 1 81 4.2e-006 +1Score > 41 indicates identity
Score > 39 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
2579 +1 570.2919 1707.8539 1707.8315 13.1 1 28 0.099 +1Score > 41 indicates identity
Score > 31 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)

+35 subsets and intersections (853 subset proteins in total)

+5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
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