MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 240)

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+31

Accession Score Description
1 STHA_PSEPG 155 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+32

Accession Score Description
1 GUAA_CELJU 155 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+33

Accession Score Description
1 PNP_PSEPK 150 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1

+34

Accession Score Description
1 AMPA_PSEPG 149 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+35

Accession Score Description
1 ATPG_PSEPG 148 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+36

Accession Score Description
1 ODO2_PSEAE 136 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

-37

Accession Score Description
1 RL5_PSEE4 134 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
Score Mass Matches Sequences emPAI
37.1 RL5_PSEE4 134 20385 10 (6) 6 (3) 0.54
50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
4 samesets of RL5_PSEE4
RL5_PSEP1 134 20316 10 (6) 6 (3) 0.55
50S ribosomal protein L5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplE PE=3 SV=1
RL5_PSEPG 134 20316 10 (6) 6 (3) 0.55
50S ribosomal protein L5 OS=Pseudomonas putida (strain GB-1) GN=rplE PE=3 SV=1
RL5_PSEPK 134 20316 10 (6) 6 (3) 0.55
50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1
RL5_PSEPW 134 20399 10 (6) 6 (3) 0.54
50S ribosomal protein L5 OS=Pseudomonas putida (strain W619) GN=rplE PE=3 SV=1

-10 peptide matches (7 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
345   340.6808 679.3470 679.3442 4.20 1 13 3.9 +9Score > 40 indicates identity
Score > 31 indicates homology		 K.FPFRN.-
492   375.7308 749.4470 749.4436 4.66 1 24 0.3 +1Score > 36 indicates identity
Score > 31 indicates homology		 R.KSIAGFK.V
782 +1 441.7964 881.5782 881.5698 9.57 0 48 0.00057 +1Score > 28 indicates identity U R.LLAISLPR.V
786   443.2473 884.4800 884.4756 5.03 1 21 0.24 +1Score > 39 indicates identity
Score > 27 indicates homology		 R.EGWPIGVK.V
1409 +1 581.3318 1160.6490 1160.6401 7.70 0 48 0.0075 +1Score > 39 indicates identity U R.GLDITLTTTAR.S
1740   658.8909 1315.7672 1315.7612 4.57 0 52 0.00088 +1Score > 34 indicates identity U K.ITGQKPVVTFAR.K
1742 +1 439.5972 1315.7698 1315.7612 6.49 0 62 2e-005 +1Score > 34 indicates identity
Score > 27 indicates homology		 U K.ITGQKPVVTFAR.K

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
RL5_AZOVD 50 20433 37.1
50S ribosomal protein L5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplE PE=3 SV=1
6 samesets of RL5_AZOVD
RL5_PSEU5 50 20384
50S ribosomal protein L5 OS=Pseudomonas stutzeri (strain A1501) GN=rplE PE=3 SV=1
RL5_PSEA7 50 20393
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain PA7) GN=rplE PE=3 SV=1
RL5_PSEA8 50 20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplE PE=3 SV=1
RL5_PSEAB 50 20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplE PE=3 SV=1
RL5_PSEAE 50 20379
50S ribosomal protein L5 OS=Pseudomonas aeruginosa GN=rplE PE=3 SV=1
RL5_PSEMY 50 20437
50S ribosomal protein L5 OS=Pseudomonas mendocina (strain ymp) GN=rplE PE=3 SV=1

+38

Accession Score Description
1 CISY_PSEAE 132 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+39

Accession Score Description
1 RL21_PSEE4 130 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+40

Accession Score Description
1 RL2_PSEP1 129 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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