MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 240)

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+31

Accession Score Description
1 STHA_PSEPG 155 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+32

Accession Score Description
1 GUAA_CELJU 155 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+33

Accession Score Description
1 PNP_PSEPK 150 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1

-34

Accession Score Description
1 AMPA_PSEPG 149 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1
Score Mass Matches Sequences emPAI
34.1 AMPA_PSEPG 149 52694 7 (6) 6 (5) 0.29
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1
1 sameset of AMPA_PSEPG
AMPA_PSEPK 149 52857 7 (6) 6 (5) 0.29
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
466   732.4307 731.4234 731.4177 7.77 0 63 0.00029 +1Score > 40 indicates identity U K.SVAAASVK.T
501   380.2357 758.4568 758.4538 4.02 0 41 0.035 +1Score > 40 indicates identity
Score > 39 indicates homology		 U K.VTLLADK.A
537   386.7522 771.4898 771.4854 5.71 0 22 1.3 +2Score > 37 indicates identity
Score > 35 indicates homology		 R.VLLVGSGK.E
1499   401.2501 1200.7285 1200.7230 4.53 1 34 0.025 +1Score > 32 indicates identity
Score > 31 indicates homology		 U K.KADKPFVLVGK.G
1652   635.3656 1268.7166 1268.7088 6.15 1 38 0.05 +1Score > 38 indicates identity U K.TATLVIPVGENR.K
2894   942.5082 1883.0018 1882.9748 14.3 0 97 8.7e-009 +1Score > 38 indicates identity
Score > 29 indicates homology		 U K.GLNGADAVLALDDIAVSNR.D
2895   629.0060 1883.9962 1883.9588 19.8 0 41 0.00068 +1Score > 38 indicates identity
Score > 21 indicates homology		 U K.GLNGADAVLALDDIAVSNR.D + Deamidated (NQ)

3 subsets and intersections (5 subset proteins in total)

Score Mass Subset of
AMPA_PSEP1 65 52796 34.1
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pepA PE=3 SV=1
1 sameset of AMPA_PSEP1
AMPA_PSEPU 65 52835
Cytosol aminopeptidase OS=Pseudomonas putida GN=pepA PE=3 SV=1
AMPA_PSEPW 63 52844 34.1
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain W619) GN=pepA PE=3 SV=1
GLMM_COLP3 41 47777 34.1
Phosphoglucosamine mutase OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=glmM PE=3 SV=1
1 sameset of GLMM_COLP3
PTHB1_MOUSE 41 100192
Protein PTHB1 OS=Mus musculus GN=Bbs9 PE=2 SV=2

+35

Accession Score Description
1 ATPG_PSEPG 148 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+36

Accession Score Description
1 ODO2_PSEAE 136 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+37

Accession Score Description
1 RL5_PSEE4 134 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+38

Accession Score Description
1 CISY_PSEAE 132 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+39

Accession Score Description
1 RL21_PSEE4 130 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+40

Accession Score Description
1 RL2_PSEP1 129 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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