MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 240)


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+31

Accession Score Description
1 STHA_PSEPG 155 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1

+32

Accession Score Description
1 GUAA_CELJU 155 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

-33

Accession Score Description
1 PNP_PSEPK 150 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
Score Mass Matches Sequences emPAI
33.1 PNP_PSEPK 150 75192 8 (6) 7 (5) 0.20
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
2 samesets of PNP_PSEPK
PNP_PSEPU 150 75166 8 (6) 7 (5) 0.20
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida GN=pnp PE=3 SV=1
PNP_PSEP1 150 75139 8 (6) 7 (5) 0.20
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pnp PE=3 SV=1

-8 peptide matches (8 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1223   538.2999 1074.5852 1074.5743 10.2 0 18 0.17 +1Score > 40 indicates identity
Score > 23 indicates homology
K.GVTALQMDIK.I
1949   707.3741 1412.7336 1412.7082 18.0 0 19 1.3 +3Score > 40 indicates identity
Score > 33 indicates homology
U K.DGLVHISMLSDAR.V
2029   726.3715 1450.7284 1450.7245 2.72 1 23 0.031 +1Score > 41 indicates identity
Score > 20 indicates homology
U K.GFFPLSVHYQEK.T
2090   743.9214 1485.8282 1485.8151 8.84 1 91 2.2e-007 +1Score > 37 indicates identity U R.GETQALVVATLGTAR.D
2094   745.4352 1488.8558 1488.8341 14.6 0 44 0.00057 +1Score > 34 indicates identity
Score > 25 indicates homology
U R.IVDFGAFVNILPGK.D
2196   767.9763 1533.9380 1533.9243 8.99 1 24 0.032 +1Score > 25 indicates identity
Score > 21 indicates homology
U K.TVRPLNIEVGVLPK.T
2197   512.3205 1533.9397 1533.9243 10.1 1 29 0.021 +1Score > 25 indicates identity U K.TVRPLNIEVGVLPK.T
2520   835.9640 1669.9134 1669.8934 12.0 0 71 6.5e-006 +1Score > 37 indicates identity
Score > 31 indicates homology
U R.LNILGQMNQVIGQSR.T

6 subsets and intersections (33 subset proteins in total)

Score Mass Subset of
PNP_PSE14 109 75121 33.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=pnp PE=3 SV=1
8 samesets of PNP_PSE14
PNP_PSEE4 109 75140
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas entomophila (strain L48) GN=pnp PE=3 SV=1
PNP_PSEFS 109 75117
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain SBW25) GN=pnp PE=3 SV=1
PNP_PSEPF 109 75026
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf0-1) GN=pnp PE=3 SV=1
PNP_PSEPG 109 75207
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1
PNP_PSEPW 109 75250
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain W619) GN=pnp PE=3 SV=1
PNP_PSESM 109 75108
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. tomato GN=pnp PE=3 SV=1
PNP_PSEU2 109 75123
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=pnp PE=3 SV=1
PNP_PSEF5 109 75343
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=pnp PE=3 SV=1
PNP_PSEA7 105 75691 33.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain PA7) GN=pnp PE=3 SV=1
3 samesets of PNP_PSEA7
PNP_PSEAB 105 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=pnp PE=3 SV=1
PNP_PSEAE 105 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa GN=pnp PE=3 SV=1
PNP_PSEA8 105 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain LESB58) GN=pnp PE=3 SV=1
PNP_AZOVD 104 75912 33.1
Polyribonucleotide nucleotidyltransferase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=pnp PE=3 SV=1
2 samesets of PNP_AZOVD
PNP_PSEMY 104 75677
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas mendocina (strain ymp) GN=pnp PE=3 SV=2
PNP_PSEU5 104 75420
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=pnp PE=3 SV=1
PNP_POLNS 91 77289 33.1
Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter necessarius (strain STIR1) GN=pnp PE=3 SV=1
1 sameset of PNP_POLNS
PNP_POLSQ 91 77293
Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=pnp PE=3 SV=1
PNP_MARAV 44 78217 33.1
Polyribonucleotide nucleotidyltransferase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=pnp PE=3 SV=1
13 samesets of PNP_MARAV
PNP_PSEHT 44 75667
Polyribonucleotide nucleotidyltransferase OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=pnp PE=3 SV=1
PNP_SHEAM 44 75438
Polyribonucleotide nucleotidyltransferase OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=pnp PE=3 SV=1
PNP_SHEFN 44 75279
Polyribonucleotide nucleotidyltransferase OS=Shewanella frigidimarina (strain NCIMB 400) GN=pnp PE=3 SV=1
PNP_SHEON 44 75605
Polyribonucleotide nucleotidyltransferase OS=Shewanella oneidensis GN=pnp PE=3 SV=2
PNP_SHEPC 44 75520
Polyribonucleotide nucleotidyltransferase OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=pnp PE=3 SV=1
PNP_SHESA 44 75432
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain ANA-3) GN=pnp PE=3 SV=1
PNP_SHESM 44 75355
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain MR-4) GN=pnp PE=3 SV=1
PNP_SHESR 44 75341
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain MR-7) GN=pnp PE=3 SV=1
PNP_SHESW 44 75520
Polyribonucleotide nucleotidyltransferase OS=Shewanella sp. (strain W3-18-1) GN=pnp PE=3 SV=1
PNP_SHEB2 44 75322
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS223) GN=pnp PE=3 SV=1
PNP_SHEB5 44 75393
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=pnp PE=3 SV=2
PNP_SHEB8 44 75363
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS185) GN=pnp PE=3 SV=1
PNP_SHEB9 44 75393
Polyribonucleotide nucleotidyltransferase OS=Shewanella baltica (strain OS195) GN=pnp PE=3 SV=1
PNP_TERTT 24 76334 33.1
Polyribonucleotide nucleotidyltransferase OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=pnp PE=3 SV=1

+34

Accession Score Description
1 AMPA_PSEPG 149 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+35

Accession Score Description
1 ATPG_PSEPG 148 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+36

Accession Score Description
1 ODO2_PSEAE 136 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+37

Accession Score Description
1 RL5_PSEE4 134 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+38

Accession Score Description
1 CISY_PSEAE 132 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+39

Accession Score Description
1 RL21_PSEE4 130 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+40

Accession Score Description
1 RL2_PSEP1 129 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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