MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 240)

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-31

Accession Score Description
1 STHA_PSEPG 155 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1
Score Mass Matches Sequences emPAI
31.1 STHA_PSEPG 155 51202 10 (5) 8 (5) 0.24
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain GB-1) GN=sthA PE=3 SV=1
2 samesets of STHA_PSEPG
STHA_PSEPK 155 51159 10 (5) 8 (5) 0.24
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain KT2440) GN=sthA PE=3 SV=3
STHA_PSEP1 155 51159 10 (5) 8 (5) 0.24
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sthA PE=3 SV=1

-10 peptide matches (10 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
210   302.6880 603.3614 603.3592 3.79 0 13 1.2 +5Score > 40 indicates identity
Score > 27 indicates homology		 E.ISSIGK.N
1029   489.7560 977.4974 977.4930 4.52 0 35 0.0042 +1Score > 41 indicates identity
Score > 24 indicates homology		 U R.VAAYDGLNR.L
1351   569.8077 1137.6008 1137.5859 13.1 0 14 0.37 +1Score > 39 indicates identity
Score > 22 indicates homology		 U R.WFSFPDVLK.S
1609   621.8334 1241.6522 1241.6438 6.82 0 18 0.45 +1Score > 40 indicates identity
Score > 27 indicates homology		 U R.AQIAGEPQGMLK.I
1938   469.6086 1405.8040 1405.7929 7.87 1 34 0.0092 +1Score > 34 indicates identity
Score > 26 indicates homology		 U R.VEGLDNGVILHLK.S
2571   852.4326 1702.8506 1702.8526 -1.16 0 49 0.00034 +1Score > 40 indicates identity
Score > 26 indicates homology		 U R.VYDSDTILSLSHTPR.K
2572   568.6254 1702.8544 1702.8526 1.03 0 11 0.33 +3Score > 40 indicates identity
Score > 19 indicates homology		 U R.VYDSDTILSLSHTPR.K
3345   717.3893 2149.1461 2149.1307 7.16 1 4 0.54 +1Score > 37 indicates identity
Score > 14 indicates homology		 U R.FVNDVPTGIYTIPEISSIGK.N
3346   1076.0797 2150.1448 2150.1147 14.0 1 31 0.0019 +1Score > 37 indicates identity
Score > 16 indicates homology		 U R.FVNDVPTGIYTIPEISSIGK.N + Deamidated (NQ)
4121   918.8047 2753.3923 2753.3409 18.7 0 99 1.4e-009 +1Score > 37 indicates identity
Score > 23 indicates homology		 U R.TTVPNIYGAGDVIGWPSLASAAHDQGR.S + Deamidated (NQ)

7 subsets and intersections (17 subset proteins in total)

Score Mass Subset of
STHA_PSEPW 79 51152 31.1
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas putida (strain W619) GN=sthA PE=3 SV=1
STHA_PSEE4 69 51180 31.1
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas entomophila (strain L48) GN=sthA PE=3 SV=1
STHA_PSEA7 68 51343 31.1
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain PA7) GN=sthA PE=3 SV=1
3 samesets of STHA_PSEA7
STHA_PSEAB 68 51357
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sthA PE=3 SV=1
STHA_PSEAE 68 51357
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa GN=sthA PE=1 SV=2
STHA_PSEA8 68 51357
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain LESB58) GN=sthA PE=3 SV=1
STHA_PSEF5 46 51067 31.1
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sthA PE=3 SV=1
1 sameset of STHA_PSEF5
STHA_PSEPF 46 51201
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens (strain Pf0-1) GN=sthA PE=3 SV=1
STHA_AZOVD 44 51527 31.1
Soluble pyridine nucleotide transhydrogenase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=sthA PE=3 SV=1
5 samesets of STHA_AZOVD
STHA_PSEFL 44 51318
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens GN=sthA PE=1 SV=3
STHA_PSEFS 44 51305
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas fluorescens (strain SBW25) GN=sthA PE=3 SV=1
STHA_PSEU2 44 51036
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sthA PE=3 SV=2
STHA_PSE14 44 51049
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sthA PE=3 SV=1
STHA_AZOVI 44 51527
Soluble pyridine nucleotide transhydrogenase OS=Azotobacter vinelandii GN=sthA PE=1 SV=1
STHA_PSESM 35 50959 31.1
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas syringae pv. tomato GN=sthA PE=3 SV=3
STHA_PSEMY 29 51268 31.1
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas mendocina (strain ymp) GN=sthA PE=3 SV=1
1 sameset of STHA_PSEMY
STHA_PSEU5 29 51637
Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas stutzeri (strain A1501) GN=sthA PE=3 SV=1

+32

Accession Score Description
1 GUAA_CELJU 155 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+33

Accession Score Description
1 PNP_PSEPK 150 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1

+34

Accession Score Description
1 AMPA_PSEPG 149 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1

+35

Accession Score Description
1 ATPG_PSEPG 148 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+36

Accession Score Description
1 ODO2_PSEAE 136 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+37

Accession Score Description
1 RL5_PSEE4 134 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+38

Accession Score Description
1 CISY_PSEAE 132 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+39

Accession Score Description
1 RL21_PSEE4 130 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+40

Accession Score Description
1 RL2_PSEP1 129 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1
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