MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)

Page: 1 2 3 4 5 6 … 24 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
CH60_PSEPK 849 56765 52 (30) 21 (11) 1.12
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
CH602_SORC5 95 58067 7 (4) 3 (2) 0.12
60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
1 sameset of CH602_SORC5
CH60_GEOLS 95 58655 10 (4) 5 (2) 0.12
60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

-53 peptide matches (37 non-duplicate, 16 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
127   487.3270 486.3197 486.3166 6.48 0 24 1.5 +4Score > 46 indicates identity
Score > 39 indicates homology		 X K.VAAVK.A
165   559.3839 558.3766 558.3741 4.55 0 32 0.086 +1Score > 34 indicates identity X R.VILSK.E
178   586.3575 585.3502 585.3486 2.77 0 39 0.059 +1Score > 39 indicates identity X K.ATLGPK.G
201 +1 301.6858 601.3570 601.3547 3.84 0 32 0.18 +1Score > 43 indicates identity
Score > 37 indicates homology		 X K.ISNIR.E
273   322.2117 642.4088 642.4064 3.74 0 9 0.89 +1Score > 41 indicates identity
Score > 21 indicates homology		 U X R.NVVLAK.S
274   643.4162 642.4089 642.4064 3.85 0 35 0.26 +4Score > 41 indicates identity U X R.NVVLAK.S
279   322.7089 643.4032 643.4017 2.42 1 7 1.2 +5Score > 44 indicates identity
Score > 20 indicates homology		 X R.VKQIR.A + Deamidated (NQ)
293   652.3083 651.3010 651.2976 5.20 0 19 1 +3Score > 40 indicates identity
Score > 32 indicates homology		 X K.FGDSAR.K
294   326.6584 651.3022 651.2976 7.08 0 19 0.68 +1Score > 40 indicates identity
Score > 30 indicates homology		 X K.FGDSAR.K
341   338.1873 674.3600 674.3599 0.21 0 29 0.24 +1Score > 43 indicates identity
Score > 36 indicates homology		 X K.DGVSVAK.E
342   675.3700 674.3627 674.3599 4.17 0 42 0.063 +1Score > 43 indicates identity X K.DGVSVAK.E
430 +1 360.1782 718.3418 718.3398 2.79 0 25 2.3 +4Score > 43 indicates identity
Score > 41 indicates homology		 X X K.APGFGDR.R
431   719.3493 718.3420 718.3398 3.03 0 32 0.2 +1Score > 43 indicates identity
Score > 37 indicates homology		 X X K.APGFGDR.R
531   386.2220 770.4294 770.4286 1.06 1 30 0.17 +1Score > 39 indicates identity
Score > 35 indicates homology		 U X R.AVESPLR.Q
532   771.4387 770.4314 770.4286 3.62 1 31 0.34 +1Score > 39 indicates identity U X R.AVESPLR.Q
652   827.5385 826.5312 826.5276 4.34 0 43 0.011 +2Score > 36 indicates identity U X K.LAGGVAVIK.V
653 +2 414.2732 826.5318 826.5276 5.10 0 53 0.0012 +1Score > 36 indicates identity U X K.LAGGVAVIK.V
877 +2 461.2575 920.5004 920.4967 4.03 0 34 0.14 +1Score > 40 indicates identity
Score > 38 indicates homology		 U X K.SFGAPTITK.D
879   921.5087 920.5014 920.4967 5.09 0 53 0.0028 +1Score > 40 indicates identity U X K.SFGAPTITK.D
898   927.5946 926.5873 926.5800 7.87 0 65 4.5e-005 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
901 +2 464.3025 926.5904 926.5800 11.2 0 54 0.00058 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
1011   972.5755 971.5682 971.5651 3.19 1 76 1.5e-005 +1Score > 40 indicates identity U X K.ATAAVVAELK.N
1013 +2 486.7926 971.5706 971.5651 5.68 1 65 0.00011 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X K.ATAAVVAELK.N
1096   506.2663 1010.5180 1010.5145 3.52 1 64 0.00021 +1Score > 40 indicates identity U X X R.VEDALHATR.A
1098 +1 337.8468 1010.5186 1010.5145 4.03 1 61 4.3e-005 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X X R.VEDALHATR.A
1762   661.8298 1321.6450 1321.6336 8.65 1 56 0.00015 +1Score > 41 indicates identity
Score > 30 indicates homology		 U X K.DAFENMGAQLVK.E
1777 +2 664.8926 1327.7706 1327.7534 13.0 0 69 3e-005 +1Score > 36 indicates identity U X K.MLVGVNVLADAVK.A
1948   707.3129 1412.6112 1412.6055 4.04 1 42 0.025 +1Score > 38 indicates identity U X R.AQIENTTSDYDR.E + Deamidated (NQ)
2148 +2 757.3894 1512.7642 1512.7532 7.29 1 94 2.1e-007 +1Score > 40 indicates identity
Score > 40 indicates homology		 U X K.GDNEDQNVGIALLR.R
2150   505.2624 1512.7654 1512.7532 8.03 1 37 0.049 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X K.GDNEDQNVGIALLR.R
2392   807.8984 1613.7822 1613.7897 -4.60 0 58 2.8e-005 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X R.QITANAGDEPSVVADK.V
2401   808.4061 1614.7976 1614.7737 14.8 0 71 1.4e-006 +1Score > 41 indicates identity
Score > 25 indicates homology		 U X R.QITANAGDEPSVVADK.V + Deamidated (NQ)
2818   614.6612 1840.9618 1840.9530 4.74 1 55 1.8e-005 +1Score > 39 indicates identity
Score > 20 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q
2819   921.4943 1840.9740 1840.9530 11.4 1 84 5.4e-008 +1Score > 38 indicates identity
Score > 24 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_5_20250714121805.raw

Score > 39 indicates identity

Score > 22 indicates homology
2820   614.9958 1841.9656 1841.9371 15.5 1 52 5.1e-005 -1Score > 39 indicates identity
Score > 22 indicates homology		 U X R.QITANAGDEPSVVADKVK.Q + Deamidated (NQ)
15.5 1 52 5.1e-005 1 QITANAGDEPSVVANKVK   + 2 Deamidated (NQ)
15.5 1 42 0.00047 3 X R.QITANAGDEPSVVADKVK.Q + Deamidated (NQ)
15.5 1 42 0.00047 3 QITANAGDEPSVVANKVK   + 2 Deamidated (NQ)
11.5 1 6 1.9 5 MPLGTVVIDEATGKPVAE   + Oxidation (M)
15.5 1 6 1.9 6 NSTAGLLDGLELGSAEPAK  
3780   801.4190 2401.2352 2401.1973 15.8 1 56 0.00081 +1Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)
3782 +1 801.4221 2401.2445 2401.1973 19.7 1 81 2.3e-006 +1Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)

+34 subsets and intersections (595 subset proteins in total)

+4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
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