MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 240)

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+21

Accession Score Description
1 DLDH2_PSEPU 211 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+22

Accession Score Description
1 RL18_PSEP1 196 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+23

Accession Score Description
1 ATPF_PSEPK 187 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+24

Accession Score Description
1 ODP2_PSEAE 183 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+25

Accession Score Description
1 KATG_PSEPK 181 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEP1 177 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

-27

Accession Score Description
1 RPOA_PSEPK 167 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
Score Mass Matches Sequences emPAI
27.1 RPOA_PSEPK 167 36721 11 (8) 8 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
2 samesets of RPOA_PSEPK
RPOA_PSEP1 167 36721 11 (8) 8 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoA PE=3 SV=1
RPOA_PSEE4 167 36721 11 (8) 8 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas entomophila (strain L48) GN=rpoA PE=3 SV=1

-11 peptide matches (10 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1003 +1 485.7848 969.5550 969.5495 5.75 1 38 0.066 +1Score > 39 indicates identity U K.ITLEPLER.G
1152   517.7874 1033.5602 1033.5556 4.48 1 45 0.0039 +1Score > 41 indicates identity
Score > 34 indicates homology		 U R.IAYVVENAR.V
1353   570.8094 1139.6042 1139.5975 5.93 0 35 0.053 +1Score > 39 indicates identity
Score > 34 indicates homology		 U R.LDNWPPASLK.K
1356   571.8088 1141.6030 1141.5992 3.33 0 44 0.00086 +1Score > 40 indicates identity
Score > 25 indicates homology		 U R.GFGHTLGNALR.R
1357   381.5417 1141.6033 1141.5992 3.53 0 40 0.0053 +1Score > 40 indicates identity
Score > 30 indicates homology		 U R.GFGHTLGNALR.R
1574   616.8415 1231.6684 1231.6561 10.0 0 57 0.00027 +1Score > 40 indicates identity
Score > 34 indicates homology		 U R.LQLDASFSPVR.R
1625   417.5682 1249.6828 1249.6779 3.90 0 35 0.011 +1Score > 39 indicates identity
Score > 28 indicates homology		 U R.HIDVQVVSPTR.A
1626   625.8493 1249.6840 1249.6779 4.92 0 65 2.1e-005 +1Score > 39 indicates identity
Score > 31 indicates homology		 U R.HIDVQVVSPTR.A
1892   463.5957 1387.7653 1387.7572 5.83 1 20 0.077 +1Score > 38 indicates identity
Score > 21 indicates homology		 U R.LQLDASFSPVRR.I
1998   717.8813 1433.7480 1433.7337 10.0 1 69 5.3e-005 +1Score > 41 indicates identity
Score > 39 indicates homology		 U -.MQISVNEFLTPR.H

7 subsets and intersections (133 subset proteins in total)

Score Mass Subset of
RPOA_PSE14 144 36765 27.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoA PE=3 SV=1
4 samesets of RPOA_PSE14
RPOA_PSEPF 144 36723
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoA PE=3 SV=1
RPOA_PSESM 144 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. tomato GN=rpoA PE=3 SV=1
RPOA_PSEU2 144 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoA PE=3 SV=1
RPOA_PSEF5 144 36721
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoA PE=3 SV=1
RPOA_PSEMY 115 36661 27.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas mendocina (strain ymp) GN=rpoA PE=3 SV=1
RPOA_PSEPU 112 36695 27.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida GN=rpoA PE=3 SV=1
RPOA_PSEU5 100 36620 27.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas stutzeri (strain A1501) GN=rpoA PE=3 SV=1
RPOA_PSEA7 83 36741 27.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain PA7) GN=rpoA PE=3 SV=1
2 samesets of RPOA_PSEA7
RPOA_PSEAB 83 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoA PE=3 SV=1
RPOA_PSEAE 83 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa GN=rpoA PE=3 SV=2
RPOA_SACD2 81 36391 27.1
DNA-directed RNA polymerase subunit alpha OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoA PE=3 SV=1
RPOA1_PSYIN 56 36427 27.1
DNA-directed RNA polymerase subunit alpha 1 OS=Psychromonas ingrahamii (strain 37) GN=rpoA1 PE=3 SV=1
+120 samesets of RPOA1_PSYIN

+28

Accession Score Description
1 RL4_PSE14 163 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+29

Accession Score Description
1 GLNA_AZOVI 158 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+30

Accession Score Description
1 ACCA_PSEPK 158 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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