MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 240)


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+21

Accession Score Description
1 DLDH2_PSEPU 211 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+22

Accession Score Description
1 RL18_PSEP1 196 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+23

Accession Score Description
1 ATPF_PSEPK 187 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+24

Accession Score Description
1 ODP2_PSEAE 183 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+25

Accession Score Description
1 KATG_PSEPK 181 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

-26

Accession Score Description
1 RL3_PSEP1 177 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1
Score Mass Matches Sequences emPAI
26.1 RL3_PSEP1 177 22703 10 (7) 7 (5) 0.98
50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1
4 samesets of RL3_PSEP1
RL3_PSEPG 177 22703 10 (7) 7 (5) 0.98
50S ribosomal protein L3 OS=Pseudomonas putida (strain GB-1) GN=rplC PE=3 SV=1
RL3_PSEPK 177 22703 10 (7) 7 (5) 0.98
50S ribosomal protein L3 OS=Pseudomonas putida (strain KT2440) GN=rplC PE=3 SV=2
RL3_PSEE4 177 22600 9 (7) 6 (5) 0.99
50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
RL3_PSEPW 177 22663 9 (7) 6 (5) 0.99
50S ribosomal protein L3 OS=Pseudomonas putida (strain W619) GN=rplC PE=3 SV=1

+10 peptide matches (10 non-duplicate, 0 duplicate)

5 subsets and intersections (24 subset proteins in total)

Score Mass Subset of
RL3_PSEF5 54 22646 26.1
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplC PE=3 SV=1
4 samesets of RL3_PSEF5
RL3_PSEFS 54 22664
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain SBW25) GN=rplC PE=3 SV=1
RL3_PSEPF 54 22603
50S ribosomal protein L3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplC PE=3 SV=1
RL3_PSESM 54 22707
50S ribosomal protein L3 OS=Pseudomonas syringae pv. tomato GN=rplC PE=3 SV=1
RL3_PSEU2 54 22693
50S ribosomal protein L3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplC PE=3 SV=1
RL3_PSEMY 53 22536 26.1
50S ribosomal protein L3 OS=Pseudomonas mendocina (strain ymp) GN=rplC PE=3 SV=1
RL3_ACTP2 51 22369 26.1
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplC PE=3 SV=1
15 samesets of RL3_ACTP2
RL3_ACTP7 51 22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 7 (strain AP76) GN=rplC PE=3 SV=1
RL3_ACTPJ 51 22369
50S ribosomal protein L3 OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=rplC PE=3 SV=1
RL3_ACTSZ 51 22312
50S ribosomal protein L3 OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=rplC PE=3 SV=1
RL3_AGGAC 51 12929
50S ribosomal protein L3 (Fragment) OS=Aggregatibacter actinomycetemcomitans GN=rplC PE=3 SV=1
RL3_BUCA5 51 22903
50S ribosomal protein L3 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A) GN=rplC PE=3 SV=1
RL3_BUCAI 51 22884
50S ribosomal protein L3 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rplC PE=3 SV=1
RL3_BUCAT 51 22903
50S ribosomal protein L3 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7) GN=rplC PE=3 SV=1
RL3_HAEDU 51 22295
50S ribosomal protein L3 OS=Haemophilus ducreyi GN=rplC PE=3 SV=1
RL3_HAEI8 51 22350
50S ribosomal protein L3 OS=Haemophilus influenzae (strain 86-028NP) GN=rplC PE=3 SV=1
RL3_HAEIG 51 22349
50S ribosomal protein L3 OS=Haemophilus influenzae (strain PittGG) GN=rplC PE=3 SV=1
RL3_HAEIN 51 22350
50S ribosomal protein L3 OS=Haemophilus influenzae GN=rplC PE=3 SV=1
RL3_HAEPS 51 22385
50S ribosomal protein L3 OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=rplC PE=3 SV=1
RL3_MANSM 51 22326
50S ribosomal protein L3 OS=Mannheimia succiniciproducens (strain MBEL55E) GN=rplC PE=3 SV=1
RL3_PASMU 51 22369
50S ribosomal protein L3 OS=Pasteurella multocida GN=rplC PE=3 SV=1
RL3_TOLAT 51 22710
50S ribosomal protein L3 OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=rplC PE=3 SV=1
RL3_PSEU5 41 22835 26.1
50S ribosomal protein L3 OS=Pseudomonas stutzeri (strain A1501) GN=rplC PE=3 SV=1
RL3_PSE14 41 22707 26.1
50S ribosomal protein L3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RPOA_PSEPK 167 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+28

Accession Score Description
1 RL4_PSE14 163 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+29

Accession Score Description
1 GLNA_AZOVI 158 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+30

Accession Score Description
1 ACCA_PSEPK 158 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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