MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 240)

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+21

Accession Score Description
1 DLDH2_PSEPU 211 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+22

Accession Score Description
1 RL18_PSEP1 196 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+23

Accession Score Description
1 ATPF_PSEPK 187 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+24

Accession Score Description
1 ODP2_PSEAE 183 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

-25

Accession Score Description
1 KATG_PSEPK 181 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1
Score Mass Matches Sequences emPAI
25.1 KATG_PSEPK 181 82068 10 (7) 7 (7) 0.21
Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

-10 peptide matches (8 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1802   671.8388 1341.6630 1341.6466 12.3 0 22 0.053 +1Score > 41 indicates identity
Score > 22 indicates homology		 U R.DWWPDQLNLR.I
2327   794.9104 1587.8062 1587.7992 4.46 1 66 4.4e-006 +1Score > 41 indicates identity
Score > 25 indicates homology		 U K.ADPSTISEQDIATLK.S
2513 +1 557.3367 1668.9883 1668.9662 13.2 1 23 0.14 +1Score > 30 indicates identity
Score > 26 indicates homology		 U K.VSLADLIVLAGTAAVEK.A
2515 +1 835.5033 1668.9920 1668.9662 15.5 1 42 0.003 +1Score > 30 indicates identity U K.VSLADLIVLAGTAAVEK.A
2569   851.9339 1701.8532 1701.8362 10.0 0 53 0.00011 +1Score > 40 indicates identity
Score > 26 indicates homology		 U R.FAPLNSWPDNVSLDK.A
2942   639.0038 1913.9896 1913.9523 19.4 0 43 0.00079 +1Score > 39 indicates identity
Score > 25 indicates homology		 U K.LGVFTDKPGTLSNDFFR.N + Deamidated (NQ)
3037   990.1177 1978.2208 1978.1826 19.3 1 48 0.0001 +1Score > 20 indicates identity U K.AQLLTLTAPELTVLIGGLR.V
4393   1018.4906 3052.4500 3052.4066 14.2 0 51 4.8e-005 +1Score > 37 indicates identity
Score > 20 indicates homology		 U K.DLTALMTDSQDWWPADFGHYGPLFIR.M + Deamidated (NQ)

9 subsets and intersections (93 subset proteins in total)

Score Mass Subset of
KATG_PSEP1 163 82012 25.1
Catalase-peroxidase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=katG PE=3 SV=1
KATG_PSEPG 99 81707 25.1
Catalase-peroxidase OS=Pseudomonas putida (strain GB-1) GN=katG PE=3 SV=1
KATG_PSEE4 81 81089 25.1
Catalase-peroxidase OS=Pseudomonas entomophila (strain L48) GN=katG PE=3 SV=1
KATG_PSEF5 77 79577 25.1
Catalase-peroxidase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=katG PE=3 SV=1
KATG_PSEPW 77 82111 25.1
Catalase-peroxidase OS=Pseudomonas putida (strain W619) GN=katG PE=3 SV=1
KATG1_BURCA 53 79222 25.1
Catalase-peroxidase 1 OS=Burkholderia cenocepacia (strain AU 1054) GN=katG1 PE=3 SV=1
+81 samesets of KATG1_BURCA
KATG_AZOC5 48 81908 25.1
Catalase-peroxidase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=katG PE=3 SV=1
2 samesets of KATG_AZOC5
KATG_FLAPJ 48 81244
Catalase-peroxidase OS=Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) GN=katG PE=3 SV=1
KATG_RALPJ 48 78627
Catalase-peroxidase OS=Ralstonia pickettii (strain 12J) GN=katG PE=3 SV=1
KATG_PYRTR 42 84619 25.1
Catalase-peroxidase OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) GN=katG PE=3 SV=1
KATG_AGRRK 22 80549 25.1
Catalase-peroxidase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) GN=katG PE=3 SV=1
1 sameset of KATG_AGRRK
KATG_SYMTH 22 82020
Catalase-peroxidase OS=Symbiobacterium thermophilum GN=katG PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEP1 177 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RPOA_PSEPK 167 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+28

Accession Score Description
1 RL4_PSE14 163 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+29

Accession Score Description
1 GLNA_AZOVI 158 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+30

Accession Score Description
1 ACCA_PSEPK 158 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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