MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 240)


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+21

Accession Score Description
1 DLDH2_PSEPU 211 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+22

Accession Score Description
1 RL18_PSEP1 196 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+23

Accession Score Description
1 ATPF_PSEPK 187 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

-24

Accession Score Description
1 ODP2_PSEAE 183 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2
Score Mass Matches Sequences emPAI
24.1 ODP2_PSEAE 183 56788 10 (6) 8 (5) 0.22
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

-10 peptide matches (8 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
119 +1 473.3112 472.3039 472.3009 6.33 0 22 1.4 +3Score > 45 indicates identity
Score > 36 indicates homology
K.AGVVK.S
231   309.1857 616.3568 616.3544 3.97 1 30 0.067 +1Score > 44 indicates identity
Score > 31 indicates homology
M.SELIR.V
619   403.7368 805.4590 805.4559 3.94 0 19 0.48 +1Score > 40 indicates identity
Score > 29 indicates homology
U K.VHAGPAVR.M
681   421.7477 841.4808 841.4770 4.60 0 56 0.0011 +1Score > 39 indicates identity U R.VINGAAAAR.F
1093 +1 505.3610 1008.7074 1008.6947 12.7 0 51 9.2e-006 +1Score > 13 indicates identity U K.LTVLPILLK.A
1512   403.8902 1208.6488 1208.6448 3.29 0 53 0.00049 +1Score > 39 indicates identity
Score > 32 indicates homology
U R.LMQVGAANLHR.S
2018   482.2637 1443.7693 1443.7544 10.3 0 59 7.2e-005 +1Score > 40 indicates identity
Score > 30 indicates homology
U R.LMLPLSLSYDHR.V
2045   731.3691 1460.7236 1460.7147 6.12 1 67 3e-005 +1Score > 41 indicates identity
Score > 34 indicates homology
U K.ELPDFNSSLAPSGK.A

2 subsets and intersections (2 subset proteins in total)

Score Mass Subset of
ODP2_AZOVI 126 65119 24.1
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Azotobacter vinelandii PE=1 SV=3
ODP2_BUCBP 59 46148 24.1
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=aceF PE=3 SV=1

+25

Accession Score Description
1 KATG_PSEPK 181 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEP1 177 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RPOA_PSEPK 167 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+28

Accession Score Description
1 RL4_PSE14 163 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+29

Accession Score Description
1 GLNA_AZOVI 158 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+30

Accession Score Description
1 ACCA_PSEPK 158 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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