MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 231–240 (out of 240)


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+231

Accession Score Description
1 RPA34_SCHPO 21 DNA-directed RNA polymerase I subunit rpa34 OS=Schizosaccharomyces pombe GN=rpa34 PE=1 SV=1

+232

Accession Score Description
1 GATC_SYNPW 21 Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C OS=Synechococcus sp. (strain WH7803) GN=gatC PE=3 SV=1

+233

Accession Score Description
1 LRG1_YEAST 20 Rho-GTPase-activating protein LRG1 OS=Saccharomyces cerevisiae GN=LRG1 PE=1 SV=2

+234

Accession Score Description
1 DNAK_PHOLL 19 Chaperone protein dnaK OS=Photorhabdus luminescens subsp. laumondii GN=dnaK PE=2 SV=1

+235

Accession Score Description
1 T2R38_GORGO 18 Taste receptor type 2 member 38 OS=Gorilla gorilla gorilla GN=TAS2R38 PE=3 SV=1

+236

Accession Score Description
1 5N3LA_XENLA 18 Cytosolic 5'-nucleotidase III-like protein A OS=Xenopus laevis GN=nt5c3l-A PE=2 SV=2

-237

Accession Score Description
1 DRL17_ARATH 18 Probable disease resistance protein At1g61300 OS=Arabidopsis thaliana GN=At1g61300 PE=2 SV=2
Score Mass Matches Sequences emPAI
237.1 DRL17_ARATH 18 88103 3 (1) 2 (1) 0.03
Probable disease resistance protein At1g61300 OS=Arabidopsis thaliana GN=At1g61300 PE=2 SV=2

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2348   533.9421 1598.8045 1598.8021 1.46 1 8 1.1 +2Score > 40 indicates identity
Score > 21 indicates homology
U E.DRVGIMGLHGMGGVGK.T + Oxidation (M)
3573   1133.1187 2264.2228 2264.2012 9.58 1 12 0.29 +1Score > 36 indicates identity
Score > 19 indicates homology
U K.LQNLQHLAITLSAELSLNQR.L + 3 Deamidated (NQ)
3574   755.7503 2264.2291 2264.2012 12.3 1 18 0.033 +1Score > 35 indicates identity
Score > 15 indicates homology
U K.LQNLQHLAITLSAELSLNQR.L + 3 Deamidated (NQ)

+238

Accession Score Description
1 PDXH2_THICR 17 Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Thiomicrospira crunogena (strain XCL-2) GN=pdxH2 PE=3 SV=1

+239

Accession Score Description
1 NU3M_SIGOC 16 NADH-ubiquinone oxidoreductase chain 3 OS=Sigmodon ochrognathus GN=MT-ND3 PE=3 SV=1

+240

Accession Score Description
1 PYRD_DESAD 15 Dihydroorotate dehydrogenase OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763) GN=pyrD PE=3 SV=1
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