MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 221–230 (out of 240)


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+221

Accession Score Description
1 ACEA_ECOLI 25 Isocitrate lyase OS=Escherichia coli (strain K12) GN=aceA PE=1 SV=1

+222

Accession Score Description
1 MUTL_ECOBW 24 DNA mismatch repair protein mutL OS=Escherichia coli (strain K12 / BW2952) GN=mutL PE=3 SV=1

+223

Accession Score Description
1 TNP7_ECOLX 24 Transposase for transposon Tn3926 OS=Escherichia coli GN=tnpA PE=3 SV=1

+224

Accession Score Description
1 CTAQ_THEAQ 24 Thermostable carboxypeptidase 1 OS=Thermus aquaticus PE=1 SV=2

+225

Accession Score Description
1 PGK_AERHH 23 Phosphoglycerate kinase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=pgk PE=3 SV=1

+226

Accession Score Description
1 PYRF_THERP 23 Orotidine 5'-phosphate decarboxylase OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) GN=pyrF PE=3 SV=1

+227

Accession Score Description
1 RL18_GLOVI 23 50S ribosomal protein L18 OS=Gloeobacter violaceus GN=rplR PE=3 SV=1

+228

Accession Score Description
1 UGPI6_ARATH 23 Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana GN=At1g61900 PE=1 SV=1

+229

Accession Score Description
1 TRMN6_BACTN 22 tRNA (adenine-N(6)-)-methyltransferase OS=Bacteroides thetaiotaomicron GN=BT_0838 PE=3 SV=2

-230

Accession Score Description
1 RS6_AZOVD 22 30S ribosomal protein S6 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsF PE=3 SV=1
Score Mass Matches Sequences emPAI
230.1 RS6_AZOVD 22 15904 4 (1) 4 (1) 0.15
30S ribosomal protein S6 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsF PE=3 SV=1
17 samesets of RS6_AZOVD
RS6_PSE14 22 16390 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsF PE=3 SV=1
RS6_PSEE4 22 16386 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas entomophila (strain L48) GN=rpsF PE=3 SV=1
RS6_PSEF5 22 16404 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsF PE=3 SV=1
RS6_PSEFS 22 16333 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsF PE=3 SV=1
RS6_PSEMY 22 16353 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas mendocina (strain ymp) GN=rpsF PE=3 SV=1
RS6_PSEP1 22 16420 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsF PE=3 SV=1
RS6_PSEPF 22 16507 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsF PE=3 SV=1
RS6_PSEPG 22 16420 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas putida (strain GB-1) GN=rpsF PE=3 SV=1
RS6_PSEPK 22 16420 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas putida (strain KT2440) GN=rpsF PE=3 SV=1
RS6_PSEPW 22 16420 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas putida (strain W619) GN=rpsF PE=3 SV=1
RS6_PSESM 22 16390 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas syringae pv. tomato GN=rpsF PE=3 SV=1
RS6_PSEU2 22 16390 4 (1) 4 (1) 0.14
30S ribosomal protein S6 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsF PE=3 SV=1
RS6_PSEA7 22 16212 3 (1) 3 (1) 0.15
30S ribosomal protein S6 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsF PE=3 SV=1
RS6_PSEA8 22 16212 3 (1) 3 (1) 0.15
30S ribosomal protein S6 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsF PE=3 SV=1
RS6_PSEAB 22 16212 3 (1) 3 (1) 0.15
30S ribosomal protein S6 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsF PE=3 SV=1
RS6_PSEAE 22 16212 3 (1) 3 (1) 0.15
30S ribosomal protein S6 OS=Pseudomonas aeruginosa GN=rpsF PE=3 SV=1
RS6_PSEU5 22 16224 3 (1) 3 (1) 0.15
30S ribosomal protein S6 OS=Pseudomonas stutzeri (strain A1501) GN=rpsF PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
219   307.7041 613.3936 613.3911 4.11 0 22 0.53 +1Score > 40 indicates identity
Score > 32 indicates homology
R.NLVIR.R
540   388.1920 774.3694 774.3660 4.40 1 16 3 +6Score > 42 indicates identity
Score > 33 indicates homology
R.LEDWGR.R
697   425.7251 849.4356 849.4344 1.41 0 32 0.076 +1Score > 42 indicates identity
Score > 34 indicates homology
R.YNDAVIR.N
1655   424.2372 1269.6898 1269.6829 5.38 0 22 0.027 +1Score > 39 indicates identity
Score > 19 indicates homology
U R.QLAYAINNVHK.A

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