MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 240)


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+21

Accession Score Description
1 DLDH2_PSEPU 211 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

-22

Accession Score Description
1 RL18_PSEP1 196 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
Score Mass Matches Sequences emPAI
22.1 RL18_PSEP1 196 12677 5 (4) 4 (3) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
3 samesets of RL18_PSEP1
RL18_PSEPG 196 12677 5 (4) 4 (3) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain GB-1) GN=rplR PE=3 SV=1
RL18_PSEPK 196 12677 5 (4) 4 (3) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1
RL18_PSEPW 196 12665 5 (4) 4 (3) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain W619) GN=rplR PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1083   502.7782 1003.5418 1003.5550 -13.1 0 22 0.93 +1Score > 41 indicates identity
Score > 34 indicates homology
K.VLASASTLDK.D
1301   560.7947 1119.5748 1119.5673 6.76 0 61 0.00017 +1Score > 42 indicates identity
Score > 35 indicates homology
U K.AAGVSQVAFDR.S
1335   567.2786 1132.5426 1132.5360 5.85 0 53 0.00037 +1Score > 41 indicates identity
Score > 31 indicates homology
U R.DGATGNIDAATK.V
1893   463.5982 1387.7728 1387.7671 4.11 1 91 1.3e-007 +1Score > 38 indicates identity
Score > 35 indicates homology
U K.VLASASTLDKDLR.D
1894   694.8939 1387.7732 1387.7671 4.45 1 96 8.7e-008 +1Score > 38 indicates identity U K.VLASASTLDKDLR.D

2 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
RL18_PSEA7 151 12711 22.1
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain PA7) GN=rplR PE=3 SV=1
4 samesets of RL18_PSEA7
RL18_TERTT 151 12760
50S ribosomal protein L18 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rplR PE=3 SV=1
RL18_PSEA8 151 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplR PE=3 SV=1
RL18_PSEAB 151 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplR PE=3 SV=1
RL18_PSEAE 151 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa GN=rplR PE=3 SV=1
RL18_PSE14 80 12653 22.1
50S ribosomal protein L18 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplR PE=3 SV=1
6 samesets of RL18_PSE14
RL18_PSEE4 80 12651
50S ribosomal protein L18 OS=Pseudomonas entomophila (strain L48) GN=rplR PE=3 SV=1
RL18_PSEF5 80 12692
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplR PE=3 SV=1
RL18_PSEFS 80 12708
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain SBW25) GN=rplR PE=3 SV=1
RL18_PSEPF 80 12692
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplR PE=3 SV=1
RL18_PSESM 80 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. tomato GN=rplR PE=3 SV=1
RL18_PSEU2 80 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplR PE=3 SV=1

+23

Accession Score Description
1 ATPF_PSEPK 187 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+24

Accession Score Description
1 ODP2_PSEAE 183 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+25

Accession Score Description
1 KATG_PSEPK 181 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEP1 177 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RPOA_PSEPK 167 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+28

Accession Score Description
1 RL4_PSE14 163 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+29

Accession Score Description
1 GLNA_AZOVI 158 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+30

Accession Score Description
1 ACCA_PSEPK 158 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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