MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 211–220 (out of 240)


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+211

Accession Score Description
1 RS5_DELAS 28 30S ribosomal protein S5 OS=Delftia acidovorans (strain DSM 14801 / SPH-1) GN=rpsE PE=3 SV=1

+212

Accession Score Description
1 PROA_PSEPG 28 Gamma-glutamyl phosphate reductase OS=Pseudomonas putida (strain GB-1) GN=proA PE=3 SV=1

+213

Accession Score Description
1 GPMI_PSEPG 28 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain GB-1) GN=gpmI PE=3 SV=1

+214

Accession Score Description
1 ARCC_PSEAE 27 Carbamate kinase OS=Pseudomonas aeruginosa GN=arcC PE=3 SV=1

+215

Accession Score Description
1 PO210_HUMAN 27 Nuclear pore membrane glycoprotein 210 OS=Homo sapiens GN=NUP210 PE=1 SV=3

+216

Accession Score Description
1 PNG1_ASHGO 26 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Ashbya gossypii GN=PNG1 PE=3 SV=1

-217

Accession Score Description
1 LPXC_AZOVD 26 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=lpxC PE=3 SV=1
Score Mass Matches Sequences emPAI
217.1 LPXC_AZOVD 26 33655 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=lpxC PE=3 SV=1
7 samesets of LPXC_AZOVD
LPXC_PSEMY 26 33573 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas mendocina (strain ymp) GN=lpxC PE=3 SV=1
LPXC_PSEPF 26 33224 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas fluorescens (strain Pf0-1) GN=lpxC PE=3 SV=1
LPXC_PSEPG 26 33264 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas putida (strain GB-1) GN=lpxC PE=3 SV=1
LPXC_PSEPK 26 33248 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas putida (strain KT2440) GN=lpxC PE=3 SV=1
LPXC_PSEPW 26 33101 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas putida (strain W619) GN=lpxC PE=3 SV=1
LPXC_PSEP1 26 33248 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=lpxC PE=3 SV=1
LPXC_PSEE4 26 33121 1 (1) 1 (1) 0.07
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Pseudomonas entomophila (strain L48) GN=lpxC PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2907   630.3743 1888.1011 1888.0822 9.98 0 26 0.013 +1Score > 29 indicates identity
Score > 20 indicates homology
U K.VYLTLKPAPVDTGIVFR.R

+218

Accession Score Description
1 APT_METJA 26 Adenine phosphoribosyltransferase OS=Methanocaldococcus jannaschii GN=apt PE=3 SV=1

+219

Accession Score Description
1 ATPF2_PROA2 25 ATP synthase subunit b 2 OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) GN=atpF2 PE=3 SV=1

+220

Accession Score Description
1 AROC_PSEAB 25 Chorismate synthase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=aroC PE=3 SV=1
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