MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 201–210 (out of 240)


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+201

Accession Score Description
1 HUTI_KLEP3 30 Imidazolonepropionase OS=Klebsiella pneumoniae (strain 342) GN=hutI PE=3 SV=1

+202

Accession Score Description
1 DNLJ_THETN 30 DNA ligase OS=Thermoanaerobacter tengcongensis GN=ligA PE=3 SV=1

+203

Accession Score Description
1 RS15_PSE14 30 30S ribosomal protein S15 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsO PE=3 SV=1

+204

Accession Score Description
1 ARGD_PSEPK 29 Acetylornithine aminotransferase OS=Pseudomonas putida (strain KT2440) GN=argD PE=3 SV=1

+205

Accession Score Description
1 BIKB_CYAP8 29 Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase OS=Cyanothece sp. (strain PCC 8801) GN=PCC8801_0067 PE=3 SV=1

+206

Accession Score Description
1 MURC_UNCTG 29 UDP-N-acetylmuramate--L-alanine ligase OS=Uncultured termite group 1 bacterium phylotype Rs-D17 GN=murC PE=3 SV=1

+207

Accession Score Description
1 ARPB_PSEPU 29 Antibiotic efflux pump membrane transporter arpB OS=Pseudomonas putida GN=arpB PE=2 SV=1

+208

Accession Score Description
1 HEAT1_DICDI 29 HEAT repeat-containing protein 1 homolog OS=Dictyostelium discoideum GN=heatr1 PE=3 SV=1

+209

Accession Score Description
1 Y146_NPVAC 28 Uncharacterized 22.9 kDa protein in IE0-IE1 intergenic region OS=Autographa californica nuclear polyhedrosis virus PE=4 SV=1

-210

Accession Score Description
1 SMC5_HUMAN 28 description
Score Mass Matches Sequences emPAI
210.1 SMC5_HUMAN 28 0 2 (1) 2 (1) 0.02
description
+76 samesets of SMC5_HUMAN

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
374   348.1724 694.3302 694.3286 2.40 0 28 0.051 +1Score > 41 indicates identity
Score > 28 indicates homology
U E.LDQYR.E + Deamidated (NQ)
1684   643.3636 1284.7126 1284.6925 15.7 1 26 1.1 +7Score > 39 indicates identity U E.LQQALIVKQNE.E + 2 Deamidated (NQ)

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