MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 21–30 (out of 240)


Page: Previous 1 2 3 4 5 6 7 8  24 Next 

-21

Accession Score Description
1 DLDH2_PSEPU 211 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
Score Mass Matches Sequences emPAI
21.1 DLDH2_PSEPU 211 50093 24 (11) 16 (9) 0.64
Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

-24 peptide matches (22 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
127   487.3270 486.3197 486.3166 6.48 0 26 0.99 +2Score > 46 indicates identity
Score > 39 indicates homology
K.AGIVK.N
136   501.3413 500.3340 500.3322 3.62 0 34 0.085 +1Score > 42 indicates identity
Score > 36 indicates homology
K.LLAGK.K
227   308.6887 615.3628 615.3592 5.99 0 19 3.1 +7Score > 45 indicates identity
Score > 36 indicates homology
K.VIADAK.T
228   616.3703 615.3630 615.3592 6.28 0 37 0.18 +1Score > 45 indicates identity
Score > 42 indicates homology
K.VIADAK.T
236   312.1739 622.3332 622.3326 1.00 0 18 0.094 +1Score > 41 indicates identity
Score > 20 indicates homology
K.ITFDK.L
328   673.3965 672.3892 672.3806 12.8 0 26 1.5 +5Score > 42 indicates identity
Score > 41 indicates homology
U K.QGLDIK.L
381   700.4383 699.4310 699.4279 4.46 0 50 0.0068 +1Score > 41 indicates identity U K.AAQLGLK.T
382   350.7230 699.4314 699.4279 5.07 0 21 1.4 +6Score > 41 indicates identity
Score > 34 indicates homology
U K.AAQLGLK.T
445   727.4852 726.4779 726.4752 3.74 0 35 0.065 +1Score > 36 indicates identity U K.LIVAVGR.R
447 +1 364.2465 726.4784 726.4752 4.47 0 35 0.043 +1Score > 36 indicates identity
Score > 34 indicates homology
U K.LIVAVGR.R
867   919.4888 918.4815 918.4811 0.50 0 65 0.00029 +1Score > 42 indicates identity U K.ALLDSSWK.Y
869   460.2503 918.4860 918.4811 5.43 0 35 0.058 +1Score > 42 indicates identity
Score > 35 indicates homology
U K.ALLDSSWK.Y
953   475.2355 948.4564 948.4521 4.58 0 24 0.63 +1Score > 42 indicates identity
Score > 35 indicates homology
U K.MDVAAMVGR.K
1305 +1 560.8265 1119.6384 1119.6288 8.60 0 52 0.0004 +1Score > 37 indicates identity
Score > 31 indicates homology
U K.NLTGGVATLFK.A
1308   561.3213 1120.6280 1120.6128 13.6 0 25 1.2 +2Score > 38 indicates identity U K.NLTGGVATLFK.A + Deamidated (NQ)
1420   390.2088 1167.6046 1167.5996 4.22 0 34 0.0037 +1Score > 40 indicates identity
Score > 22 indicates homology
U K.ANGVTSIQGHGK.L
1421   584.8101 1167.6056 1167.5996 5.15 0 65 7.5e-006 +1Score > 40 indicates identity
Score > 26 indicates homology
U K.ANGVTSIQGHGK.L
1452   591.2870 1180.5594 1180.5547 4.06 0 66 2.5e-005 +1Score > 41 indicates identity
Score > 32 indicates homology
U R.AMAANDTGGFVK.V
1785   444.6113 1330.8121 1330.7973 11.1 1 50 0.0004 +1Score > 28 indicates identity U K.ITFDKLIVAVGR.R
2364   804.8959 1607.7772 1607.7944 -10.7 1 2 2.2 +6Score > 41 indicates identity
Score > 18 indicates homology
U E.GVEVNVGTFPFAASGR.A + Deamidated (NQ)
3212   1046.0928 2090.1710 2090.1412 14.3 1 50 0.0011 +1Score > 32 indicates identity U M.TQKFDVVVIGAGPGGYVAAIK.A + Deamidated (NQ)
4022   882.7982 2645.3728 2645.3199 20.0 1 37 0.05 +1Score > 36 indicates identity U K.AQMNYDLIPSVIYTHPEIAWVGK.T + Deamidated (NQ)

3 subsets and intersections (15 subset proteins in total)

Score Mass Subset of
DLDH2_ARATH 50 54237 21.1
Dihydrolipoyl dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=LPD2 PE=1 SV=1
3 samesets of DLDH2_ARATH
DLDH_SOLTU 50 3910
Dihydrolipoyl dehydrogenase (Fragment) OS=Solanum tuberosum PE=1 SV=1
DLDH_TRYBB 50 50815
Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei brucei PE=3 SV=1
DLDH2_BACSU 50 50632
Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=bfmBC PE=3 SV=1
DLDH2_PSEAE 48 50362 21.1
Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1
1 sameset of DLDH2_PSEAE
DLDH_PSEFL 48 50348
Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3
DFX_METTH 35 14264 21.1
Desulfoferrodoxin homolog OS=Methanobacterium thermoautotrophicum GN=MTH_757 PE=3 SV=1
8 samesets of DFX_METTH
DLDH_AZOVI 35 49707
Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1
GPMI_MAGSM 35 56808
2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Magnetococcus sp. (strain MC-1) GN=gpmI PE=3 SV=1
DLDH_THESC 35 48589
Dihydrolipoyl dehydrogenase OS=Thermus scotoductus GN=lpd PE=1 SV=2
GLMU_DECAR 35 48067
Bifunctional protein glmU OS=Dechloromonas aromatica (strain RCB) GN=glmU PE=3 SV=1
RPPH_ACICJ 35 18342
RNA pyrophosphohydrolase OS=Acidiphilium cryptum (strain JF-5) GN=rppH PE=3 SV=1
YNF1_RHOCA 35 27985
Uncharacterized 27.7 kDa protein in nifB 3'region OS=Rhodobacter capsulatus PE=4 SV=1
DLDH3_HALMA 35 50375
Dihydrolipoyl dehydrogenase 3 OS=Haloarcula marismortui GN=lpdA3 PE=3 SV=1
HSE1_SCHPO 35 42699
Class E vacuolar protein-sorting machinery protein hse1 OS=Schizosaccharomyces pombe GN=hse1 PE=2 SV=1

+22

Accession Score Description
1 RL18_PSEP1 196 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+23

Accession Score Description
1 ATPF_PSEPK 187 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+24

Accession Score Description
1 ODP2_PSEAE 183 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+25

Accession Score Description
1 KATG_PSEPK 181 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+26

Accession Score Description
1 RL3_PSEP1 177 50S ribosomal protein L3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplC PE=3 SV=1

+27

Accession Score Description
1 RPOA_PSEPK 167 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+28

Accession Score Description
1 RL4_PSE14 163 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+29

Accession Score Description
1 GLNA_AZOVI 158 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+30

Accession Score Description
1 ACCA_PSEPK 158 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
Page: Previous 1 2 3 4 5 6 7 8  24 Next 

Not what you expected? Try the select summary.