MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 191–200 (out of 240)


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+191

Accession Score Description
1 YLP3_PSEPU 32 Uncharacterized methyltransferase in lpd-3 5'region OS=Pseudomonas putida PE=4 SV=1

+192

Accession Score Description
1 MREB_BACSU 32 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+193

Accession Score Description
1 RNH3_STRP1 32 Ribonuclease HIII OS=Streptococcus pyogenes serotype M1 GN=rnhC PE=3 SV=1

+194

Accession Score Description
1 ASTB_PSEFS 31 N-succinylarginine dihydrolase OS=Pseudomonas fluorescens (strain SBW25) GN=astB PE=3 SV=1

+195

Accession Score Description
1 SSB_PSEPK 31 Single-stranded DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=ssb PE=3 SV=1

+196

Accession Score Description
1 LCP5_YEAST 31 U3 small nucleolar ribonucleoprotein protein LCP5 OS=Saccharomyces cerevisiae GN=LCP5 PE=1 SV=1

+197

Accession Score Description
1 PURT_PSESM 31 Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas syringae pv. tomato GN=purT PE=3 SV=1

+198

Accession Score Description
1 DPO3_LACC3 30 DNA polymerase III polC-type OS=Lactobacillus casei (strain ATCC 334) GN=polC PE=3 SV=1

+199

Accession Score Description
1 ACSA_ECOLI 30 Acetyl-coenzyme A synthetase OS=Escherichia coli (strain K12) GN=acs PE=1 SV=2

-200

Accession Score Description
1 ILVC_HELMI 30 Ketol-acid reductoisomerase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=ilvC PE=3 SV=1
Score Mass Matches Sequences emPAI
200.1 ILVC_HELMI 30 36747 1 (1) 1 (1) 0.06
Ketol-acid reductoisomerase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=ilvC PE=3 SV=1
6 samesets of ILVC_HELMI
MRAW_AZOSB 30 33516 1 (1) 1 (1) 0.07
S-adenosyl-L-methionine-dependent methyltransferase mraW OS=Azoarcus sp. (strain BH72) GN=mraW PE=3 SV=1
MRAW_VIBFM 30 35236 1 (1) 1 (1) 0.07
S-adenosyl-L-methionine-dependent methyltransferase mraW OS=Vibrio fischeri (strain MJ11) GN=mraW PE=3 SV=1
SECA_JANMA 30 103915 1 (1) 1 (1) 0.02
Protein translocase subunit secA OS=Janthinobacterium sp. (strain Marseille) GN=secA PE=3 SV=1
CHEB1_SYNWW 30 38556 1 (1) 1 (1) 0.06
Chemotaxis response regulator protein-glutamate methylesterase 1 OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=cheB1 PE=3 SV=1
SAS6_CAEBR 30 55928 1 (1) 1 (1) 0.04
Spindle assembly abnormal protein 6 OS=Caenorhabditis briggsae GN=sas-6 PE=3 SV=3
Y146A_HAEIN 30 38721 1 (1) 1 (1) 0.06
Uncharacterized tonB-dependent receptor HI1466.1 OS=Haemophilus influenzae GN=HI1466.1 PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
299   329.7105 657.4064 657.4061 0.51 1 30 0.041 +1Score > 41 indicates identity
Score > 29 indicates homology
U E.IEIVGK.K

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