MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 191–200 (out of 240)

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+191

Accession Score Description
1 YLP3_PSEPU 32 Uncharacterized methyltransferase in lpd-3 5'region OS=Pseudomonas putida PE=4 SV=1

+192

Accession Score Description
1 MREB_BACSU 32 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+193

Accession Score Description
1 RNH3_STRP1 32 Ribonuclease HIII OS=Streptococcus pyogenes serotype M1 GN=rnhC PE=3 SV=1

+194

Accession Score Description
1 ASTB_PSEFS 31 N-succinylarginine dihydrolase OS=Pseudomonas fluorescens (strain SBW25) GN=astB PE=3 SV=1

+195

Accession Score Description
1 SSB_PSEPK 31 Single-stranded DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=ssb PE=3 SV=1

+196

Accession Score Description
1 LCP5_YEAST 31 U3 small nucleolar ribonucleoprotein protein LCP5 OS=Saccharomyces cerevisiae GN=LCP5 PE=1 SV=1

-197

Accession Score Description
1 PURT_PSESM 31 Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas syringae pv. tomato GN=purT PE=3 SV=1
Score Mass Matches Sequences emPAI
197.1 PURT_PSESM 31 42474 2 (1) 2 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas syringae pv. tomato GN=purT PE=3 SV=1
8 samesets of PURT_PSESM
PURT_PSEFS 31 42555 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas fluorescens (strain SBW25) GN=purT PE=3 SV=1
PURT_PSEPF 31 42436 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=purT PE=3 SV=1
PURT_PSEPG 31 42760 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas putida (strain GB-1) GN=purT PE=3 SV=1
PURT_PSEPK 31 42777 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas putida (strain KT2440) GN=purT PE=3 SV=1
PURT_PSEPW 31 42759 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas putida (strain W619) GN=purT PE=3 SV=1
PURT_PSEU2 31 42488 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=purT PE=3 SV=1
PURT_PSEF5 31 42539 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=purT PE=3 SV=1
PURT_PSE14 31 42464 1 (1) 1 (1) 0.05
Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=purT PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
131   489.2804 488.2731 488.2707 4.98 0 3 5.7 +8Score > 44 indicates identity
Score > 24 indicates homology		 E.SLGGR.G
4759   762.3992 3806.9596 3806.9017 15.2 1 31 0.0039 +1Score > 33 indicates identity
Score > 19 indicates homology		 U K.GDQVWFSEVSPRPHDTGLVTLISQDLSQFALHAR.A + Deamidated (NQ)

+198

Accession Score Description
1 DPO3_LACC3 30 DNA polymerase III polC-type OS=Lactobacillus casei (strain ATCC 334) GN=polC PE=3 SV=1

+199

Accession Score Description
1 ACSA_ECOLI 30 Acetyl-coenzyme A synthetase OS=Escherichia coli (strain K12) GN=acs PE=1 SV=2

+200

Accession Score Description
1 ILVC_HELMI 30 Ketol-acid reductoisomerase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=ilvC PE=3 SV=1
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