MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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191| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YLP3_PSEPU | 32 | Uncharacterized methyltransferase in lpd-3 5'region OS=Pseudomonas putida PE=4 SV=1 |
192| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MREB_BACSU | 32 | Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3 |
193| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RNH3_STRP1 | 32 | Ribonuclease HIII OS=Streptococcus pyogenes serotype M1 GN=rnhC PE=3 SV=1 |
194| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ASTB_PSEFS | 31 | N-succinylarginine dihydrolase OS=Pseudomonas fluorescens (strain SBW25) GN=astB PE=3 SV=1 |
195| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SSB_PSEPK | 31 | Single-stranded DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=ssb PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 195.1 | SSB_PSEPK |
31 | 20161 | 2 (1) | 2 (1) | 0.12 | |
| Single-stranded DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=ssb PE=3 SV=1 | |||||||
| 7 samesets of SSB_PSEPK | |||||||
| GTPBA_DROPS |
31 | 42162 | 1 (1) | 1 (1) | 0.05 | ||
| GTP-binding protein 10 homolog OS=Drosophila pseudoobscura pseudoobscura GN=GA10450 PE=3 SV=1 | |||||||
| SSBD_PSEPU |
31 | 18357 | 1 (1) | 1 (1) | 0.13 | ||
| Single-stranded DNA-binding protein OS=Pseudomonas putida GN=ssb PE=3 SV=1 | |||||||
| SSBW_PSEPU |
31 | 20547 | 1 (1) | 1 (1) | 0.11 | ||
| Single-stranded DNA-binding protein OS=Pseudomonas putida GN=ssb PE=3 SV=1 | |||||||
| CP135_HUMAN |
31 | 133878 | 1 (1) | 1 (1) | 0.02 | ||
| Centrosomal protein of 135 kDa OS=Homo sapiens GN=CEP135 PE=1 SV=1 | |||||||
| NDC80_HUMAN |
31 | 74380 | 1 (1) | 1 (1) | 0.03 | ||
| Kinetochore protein NDC80 homolog OS=Homo sapiens GN=NDC80 PE=1 SV=1 | |||||||
| NDC80_MACFA |
31 | 74335 | 1 (1) | 1 (1) | 0.03 | ||
| Kinetochore protein NDC80 homolog OS=Macaca fascicularis GN=NDC80 PE=2 SV=1 | |||||||
| NDC80_MOUSE |
31 | 74372 | 1 (1) | 1 (1) | 0.03 | ||
| Kinetochore protein NDC80 homolog OS=Mus musculus GN=Ndc80 PE=2 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 288 |
325.6982 | 649.3818 | 649.3799 | 2.98 | 0 | 31 | 0.047 | 1Score > 40 indicates identity Score > 31 indicates homology |
U | R.VSLFGK.V | |
| 4784 |
976.9772 | 3903.8797 | 3903.8089 | 18.1 | 0 | 5 | 1 | 1Score > 34 indicates identity Score > 17 indicates homology |
U | R.QQAQRPQQAPQRPAPQQPAPQPAADFDSFDDDIPF.- + 2 Deamidated (NQ) |
196| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LCP5_YEAST | 31 | U3 small nucleolar ribonucleoprotein protein LCP5 OS=Saccharomyces cerevisiae GN=LCP5 PE=1 SV=1 |
197| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURT_PSESM | 31 | Phosphoribosylglycinamide formyltransferase 2 OS=Pseudomonas syringae pv. tomato GN=purT PE=3 SV=1 |
198| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DPO3_LACC3 | 30 | DNA polymerase III polC-type OS=Lactobacillus casei (strain ATCC 334) GN=polC PE=3 SV=1 |
199| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACSA_ECOLI | 30 | Acetyl-coenzyme A synthetase OS=Escherichia coli (strain K12) GN=acs PE=1 SV=2 |
200| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_HELMI | 30 | Ketol-acid reductoisomerase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=ilvC PE=3 SV=1 |
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