Rita5 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita5 sp
MS data file	:	PRT1270_T-BRSC_5_20250714121805.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:42:02 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita5 sp

MS data file

PRT1270_T-BRSC_5_20250714121805.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:42:02 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,997

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,997

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (

) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Protein families 11–20 (out of 240)

Previous 1 2 3 4 5 6 7 … 24 Next

11

		Accession	Score	Description
	1	DNAK_PSEPK	321	Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

12

		Accession	Score	Description
	1	DBHB_PSEAE	296	DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

13

		Accession	Score	Description
	1	EFG1_PSEPK	288	Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

14

		Accession	Score	Description
	1	HTPG_PSEPK	268	Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

15

		Accession	Score	Description
	1	SUCC_PSEPK	266	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

16

		Accession	Score	Description
	1	DHSA_ECOLI	256	Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

17

		Accession	Score	Description
	1	RISB_PSEPG	254	6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

18

		Accession	Score	Description
	1	ACON2_PSEAE	253	Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

19

		Accession	Score	Description
	1	RS5_PSEE4	226	30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
19.1	RS5_PSEE4	226	17700	11 (7)	6 (4)	0.87
	30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1
	4 samesets of RS5_PSEE4
	RS5_PSEP1	226	17712	11 (7)	6 (4)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsE PE=3 SV=1
	RS5_PSEPG	226	17712	11 (7)	6 (4)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain GB-1) GN=rpsE PE=3 SV=1
	RS5_PSEPK	226	17712	11 (7)	6 (4)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1
	RS5_PSEPW	226	17712	11 (7)	6 (4)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain W619) GN=rpsE PE=3 SV=1

11 peptide matches (8 non-duplicate, 3 duplicate)

Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
449	1	364.7257	727.4368	727.4341	3.82	0	27	0.093	1Score > 39 indicates identity Score > 29 indicates homology		K.LVQVNR.V
714		855.4961	854.4888	854.4861	3.14	1	49	0.0036	1Score > 37 indicates identity	U	R.EVPAAIQK.A
715		428.2517	854.4888	854.4861	3.16	1	17	1.3	2Score > 37 indicates identity Score > 31 indicates homology	U	R.EVPAAIQK.A
1034		491.7656	981.5166	981.5131	3.61	0	41	0.033	1Score > 39 indicates identity Score > 39 indicates homology	U	K.GTTLQYATK.A
1035		982.5247	981.5174	981.5131	4.40	0	67	9.9e-005	1Score > 39 indicates identity	U	K.GTTLQYATK.A
1332		566.7919	1131.5692	1131.5594	8.73	1	20	0.22	1Score > 42 indicates identity Score > 26 indicates homology	U	K.AMQSPESIAAK.R
1944	1	705.9254	1409.8362	1409.8242	8.54	1	65	1.4e-005	1Score > 33 indicates identity Score > 29 indicates homology	U	R.AVLEVAGVQNVLAK.C
2056	1	734.9158	1467.8170	1467.7974	13.4	0	98	1.8e-008	1Score > 37 indicates identity Score > 33 indicates homology	U	R.IFTFTALTVVGDGK.G

5 subsets and intersections (124 subset proteins in total)

	Score	Mass	Subset of
RS5_AZOVD	194	17763	19.1
30S ribosomal protein S5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsE PE=3 SV=1
7 samesets of RS5_AZOVD
RS5_PSEF5	194	17699
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsE PE=3 SV=1
RS5_PSEFS	194	17732
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsE PE=3 SV=1
RS5_PSEPF	194	17769
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsE PE=3 SV=1
RS5_PSE14	194	17881
30S ribosomal protein S5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsE PE=3 SV=1
RS5_PSEMY	194	17708
30S ribosomal protein S5 OS=Pseudomonas mendocina (strain ymp) GN=rpsE PE=3 SV=1
RS5_PSESM	194	17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. tomato GN=rpsE PE=3 SV=1
RS5_PSEU2	194	17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsE PE=3 SV=1
RS5_PSEU5	147	17649	19.1
30S ribosomal protein S5 OS=Pseudomonas stutzeri (strain A1501) GN=rpsE PE=3 SV=1
RS5_ALCBS	92	17601	19.1
30S ribosomal protein S5 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsE PE=3 SV=1
4 samesets of RS5_ALCBS
RS5_PSEA7	92	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsE PE=3 SV=1
RS5_PSEA8	92	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsE PE=3 SV=1
RS5_PSEAB	92	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsE PE=3 SV=1
RS5_PSEAE	92	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa GN=rpsE PE=3 SV=1
RS5_HAHCH	76	17639	19.1
30S ribosomal protein S5 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsE PE=3 SV=1
RS5_ACTP2	49	17463	19.1
30S ribosomal protein S5 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rpsE PE=3 SV=1
108 samesets of RS5_ACTP2

20

	Accession	Score	Description
1	PORF_PSEAE	211	Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1
2	PORF_PSESY	177	Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1

Previous 1 2 3 4 5 6 7 … 24 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 11–20 (out of 240)

11

12

13

14

15

16

17

18

19

11 peptide matches (8 non-duplicate, 3 duplicate)

5 subsets and intersections (124 subset proteins in total)

20