MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 181–190 (out of 240)

Page: Previous 1 …14 15 16 17 18 19 20 21 22 23 24 Next 

+181

Accession Score Description
1 RS10_PSEF5 35 30S ribosomal protein S10 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsJ PE=3 SV=1

+182

Accession Score Description
1 MASZ_NOCFA 35 Malate synthase G OS=Nocardia farcinica GN=glcB PE=3 SV=1

+183

Accession Score Description
1 METK_PSEPG 34 S-adenosylmethionine synthetase OS=Pseudomonas putida (strain GB-1) GN=metK PE=3 SV=1

+184

Accession Score Description
1 MFD_STAEQ 34 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1

+185

Accession Score Description
1 ECHH_RHIME 34 Probable enoyl-CoA hydratase OS=Rhizobium meliloti GN=fadB1 PE=3 SV=2

+186

Accession Score Description
1 HCYA_APHCL 34 Hemocyanin A chain OS=Aphonopelma californicum GN=HCA PE=1 SV=3

+187

Accession Score Description
1 SYL_SHEDO 33 Leucyl-tRNA synthetase OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=leuS PE=3 SV=2

+188

Accession Score Description
1 Y396_PSEPK 33 UPF0229 protein PP_0396 OS=Pseudomonas putida (strain KT2440) GN=PP_0396 PE=3 SV=1

-189

Accession Score Description
1 PUR5_PSEPF 33 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain Pf0-1) GN=purM PE=3 SV=1
Score Mass Matches Sequences emPAI
189.1 PUR5_PSEPF 33 37293 4 (1) 3 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain Pf0-1) GN=purM PE=3 SV=1
14 samesets of PUR5_PSEPF
PUR5_PSEMY 33 37182 3 (1) 2 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1
PUR5_PSEP1 33 37375 3 (1) 2 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purM PE=3 SV=1
PUR5_PSEPG 33 37375 3 (1) 2 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain GB-1) GN=purM PE=3 SV=1
PUR5_PSEPK 33 37375 3 (1) 2 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain KT2440) GN=purM PE=3 SV=1
PUR5_PSEPW 33 37493 3 (1) 2 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain W619) GN=purM PE=3 SV=1
PUR5_PSE14 33 37357 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=purM PE=3 SV=1
PUR5_PSEA8 33 37670 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas aeruginosa (strain LESB58) GN=purM PE=3 SV=1
PUR5_PSEAB 33 37670 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=purM PE=3 SV=1
PUR5_PSEAE 33 37612 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas aeruginosa GN=purM PE=3 SV=1
PUR5_PSEE4 33 37486 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas entomophila (strain L48) GN=purM PE=3 SV=1
PUR5_PSEF5 33 37377 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=purM PE=3 SV=1
PUR5_PSEFS 33 37406 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain SBW25) GN=purM PE=3 SV=1
PUR5_PSESM 33 37414 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. tomato GN=purM PE=3 SV=1
PUR5_PSEU2 33 37355 1 (1) 1 (1) 0.06
Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=purM PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
567   393.7466 785.4786 785.4759 3.47 0 13 4.4 +8Score > 41 indicates identity
Score > 32 indicates homology		 U E.VALNTLR.D
1774   443.2997 1326.8773 1326.9002 -17.3 1 33 0.00074 +1Score > 14 indicates identity U R.IYVKPLLKLIK.D
3775 +1 799.7661 2396.2765 2396.2336 17.9 0 12 0.32 +1Score > 36 indicates identity
Score > 20 indicates homology		 U K.VATGDALIALPSSGPHSNGYSLIR.K + Deamidated (NQ)

+190

Accession Score Description
1 PIFA_ECOLI 33 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2
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