MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 181–190 (out of 240)

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+181

Accession Score Description
1 RS10_PSEF5 35 30S ribosomal protein S10 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsJ PE=3 SV=1

+182

Accession Score Description
1 MASZ_NOCFA 35 Malate synthase G OS=Nocardia farcinica GN=glcB PE=3 SV=1

+183

Accession Score Description
1 METK_PSEPG 34 S-adenosylmethionine synthetase OS=Pseudomonas putida (strain GB-1) GN=metK PE=3 SV=1

+184

Accession Score Description
1 MFD_STAEQ 34 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1

+185

Accession Score Description
1 ECHH_RHIME 34 Probable enoyl-CoA hydratase OS=Rhizobium meliloti GN=fadB1 PE=3 SV=2

-186

Accession Score Description
1 HCYA_APHCL 34 Hemocyanin A chain OS=Aphonopelma californicum GN=HCA PE=1 SV=3
Score Mass Matches Sequences emPAI
186.1 HCYA_APHCL 34 72843 1 (1) 1 (1) 0.03
Hemocyanin A chain OS=Aphonopelma californicum GN=HCA PE=1 SV=3
1 sameset of HCYA_APHCL
FA26D_MOUSE 34 36375 1 (1) 1 (1) 0.06
Protein FAM26D OS=Mus musculus GN=Fam26d PE=2 SV=2

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
412   354.7307 707.4468 707.4442 3.70 0 34 0.022 +1Score > 30 indicates identity U E.VALLHR.E

+187

Accession Score Description
1 SYL_SHEDO 33 Leucyl-tRNA synthetase OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=leuS PE=3 SV=2

+188

Accession Score Description
1 Y396_PSEPK 33 UPF0229 protein PP_0396 OS=Pseudomonas putida (strain KT2440) GN=PP_0396 PE=3 SV=1

+189

Accession Score Description
1 PUR5_PSEPF 33 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain Pf0-1) GN=purM PE=3 SV=1

+190

Accession Score Description
1 PIFA_ECOLI 33 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2
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