MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 161–170 (out of 240)


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+161

Accession Score Description
1 Y041_SYNY3 41 Putative methyl-accepting chemotaxis protein sll0041 OS=Synechocystis sp. (strain PCC 6803) GN=sll0041 PE=3 SV=2

+162

Accession Score Description
1 MURC_MACCJ 40 UDP-N-acetylmuramate--L-alanine ligase OS=Macrococcus caseolyticus (strain JCSC5402) GN=murC PE=3 SV=1

+163

Accession Score Description
1 MNME_SHEON 40 tRNA modification GTPase mnmE OS=Shewanella oneidensis GN=mnmE PE=3 SV=2

+164

Accession Score Description
1 ARLY_CALS8 40 Argininosuccinate lyase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=argH PE=3 SV=1

+165

Accession Score Description
1 GLYA1_PSEF5 39 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

+166

Accession Score Description
1 MRAW_OENOB 38 S-adenosyl-L-methionine-dependent methyltransferase mraW OS=Oenococcus oeni (strain BAA-331 / PSU-1) GN=mraW PE=3 SV=1

+167

Accession Score Description
1 HEM1_DESPS 38 Glutamyl-tRNA reductase OS=Desulfotalea psychrophila GN=hemA PE=3 SV=1

-168

Accession Score Description
1 PSAB_PORPU 38 Photosystem I P700 chlorophyll a apoprotein A2 OS=Porphyra purpurea GN=psaB PE=3 SV=1
Score Mass Matches Sequences emPAI
168.1 PSAB_PORPU 38 82244 1 (1) 1 (1) 0.03
Photosystem I P700 chlorophyll a apoprotein A2 OS=Porphyra purpurea GN=psaB PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1099   337.8673 1010.5801 1010.5913 -11.1 0 38 0.019 +1Score > 36 indicates identity
Score > 33 indicates homology
U E.QWILNPLK.V

+169

Accession Score Description
1 FAK2_HUMAN 37 Protein-tyrosine kinase 2-beta OS=Homo sapiens GN=PTK2B PE=1 SV=2

+170

Accession Score Description
1 C4AD1_DROME 37 Probable cytochrome P450 4ad1 OS=Drosophila melanogaster GN=Cyp4ad1 PE=2 SV=1
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