MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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161| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Y041_SYNY3 | 41 | Putative methyl-accepting chemotaxis protein sll0041 OS=Synechocystis sp. (strain PCC 6803) GN=sll0041 PE=3 SV=2 |
162| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MURC_MACCJ | 40 | UDP-N-acetylmuramate--L-alanine ligase OS=Macrococcus caseolyticus (strain JCSC5402) GN=murC PE=3 SV=1 |
163| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MNME_SHEON | 40 | tRNA modification GTPase mnmE OS=Shewanella oneidensis GN=mnmE PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 163.1 | MNME_SHEON |
40 | 49386 | 2 (1) | 2 (1) | 0.05 | |
| tRNA modification GTPase mnmE OS=Shewanella oneidensis GN=mnmE PE=3 SV=2 | |||||||
| 5 samesets of MNME_SHEON | |||||||
| MNME_SHEPC |
40 | 49291 | 2 (1) | 2 (1) | 0.05 | ||
| tRNA modification GTPase mnmE OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=mnmE PE=3 SV=1 | |||||||
| MNME_SHESA |
40 | 49251 | 2 (1) | 2 (1) | 0.05 | ||
| tRNA modification GTPase mnmE OS=Shewanella sp. (strain ANA-3) GN=mnmE PE=3 SV=1 | |||||||
| MNME_SHESM |
40 | 49280 | 2 (1) | 2 (1) | 0.05 | ||
| tRNA modification GTPase mnmE OS=Shewanella sp. (strain MR-4) GN=mnmE PE=3 SV=1 | |||||||
| MNME_SHESR |
40 | 49309 | 2 (1) | 2 (1) | 0.05 | ||
| tRNA modification GTPase mnmE OS=Shewanella sp. (strain MR-7) GN=mnmE PE=3 SV=1 | |||||||
| MNME_SHESW |
40 | 49291 | 2 (1) | 2 (1) | 0.05 | ||
| tRNA modification GTPase mnmE OS=Shewanella sp. (strain W3-18-1) GN=mnmE PE=3 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1334 |
1132.6215 | 1131.6142 | 1131.5957 | 16.3 | 1 | 40 | 0.015 | 1Score > 41 indicates identity Score > 35 indicates homology |
U | E.LRMAQLALSE.I + Deamidated (NQ) | |
| 1725 |
653.8407 | 1305.6668 | 1305.6598 | 5.38 | 1 | 10 | 1.9 | 6Score > 42 indicates identity Score > 25 indicates homology |
U | R.MAQLALSEITGR.F + Deamidated (NQ); Oxidation (M) |
164| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARLY_CALS8 | 40 | Argininosuccinate lyase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=argH PE=3 SV=1 |
165| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLYA1_PSEF5 | 39 | Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1 |
166| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MRAW_OENOB | 38 | S-adenosyl-L-methionine-dependent methyltransferase mraW OS=Oenococcus oeni (strain BAA-331 / PSU-1) GN=mraW PE=3 SV=1 |
167| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEM1_DESPS | 38 | Glutamyl-tRNA reductase OS=Desulfotalea psychrophila GN=hemA PE=3 SV=1 |
168| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PSAB_PORPU | 38 | Photosystem I P700 chlorophyll a apoprotein A2 OS=Porphyra purpurea GN=psaB PE=3 SV=1 |
169| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FAK2_HUMAN | 37 | Protein-tyrosine kinase 2-beta OS=Homo sapiens GN=PTK2B PE=1 SV=2 |
170| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | C4AD1_DROME | 37 | Probable cytochrome P450 4ad1 OS=Drosophila melanogaster GN=Cyp4ad1 PE=2 SV=1 |
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