MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 151–160 (out of 240)

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+151

Accession Score Description
1 PHS_PSEAB 42 Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1

+152

Accession Score Description
1 ARGD_XYLFT 42 Acetylornithine aminotransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=argD PE=3 SV=1

+153

Accession Score Description
1 SYGB_SHEHH 42 Glycyl-tRNA synthetase beta subunit OS=Shewanella halifaxensis (strain HAW-EB4) GN=glyS PE=3 SV=1

+154

Accession Score Description
1 EFR3_CHAGB 42 Protein EFR3 OS=Chaetomium globosum GN=EFR3 PE=3 SV=1

+155

Accession Score Description
1 ATMB_SALTY 42 Magnesium-transporting ATPase, P-type 1 OS=Salmonella typhimurium GN=mgtB PE=2 SV=3

+156

Accession Score Description
1 THIG_AZOVD 42 Thiazole synthase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=thiG PE=3 SV=1

-157

Accession Score Description
1 SYD_METM5 42 Aspartyl-tRNA synthetase OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=aspS PE=3 SV=1
Score Mass Matches Sequences emPAI
157.1 SYD_METM5 42 50296 3 (1) 3 (1) 0.05
Aspartyl-tRNA synthetase OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=aspS PE=3 SV=1
19 samesets of SYD_METM5
SYD_METM6 42 50388 3 (1) 3 (1) 0.05
Aspartyl-tRNA synthetase OS=Methanococcus maripaludis (strain C6 / ATCC BAA-1332) GN=aspS PE=3 SV=1
SYD_METM7 42 50431 3 (1) 3 (1) 0.05
Aspartyl-tRNA synthetase OS=Methanococcus maripaludis (strain C7 / ATCC BAA-1331) GN=aspS PE=3 SV=1
RPOA_PROAC 42 36997 2 (1) 2 (1) 0.06
DNA-directed RNA polymerase subunit alpha OS=Propionibacterium acnes GN=rpoA PE=3 SV=1
BIOD_XYLFA 42 24124 1 (1) 1 (1) 0.10
Dethiobiotin synthetase OS=Xylella fastidiosa GN=bioD PE=3 SV=1
BIOD_XYLFM 42 24257 1 (1) 1 (1) 0.10
Dethiobiotin synthetase OS=Xylella fastidiosa (strain M12) GN=bioD PE=3 SV=1
BIOD_XYLFT 42 24236 1 (1) 1 (1) 0.10
Dethiobiotin synthetase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=bioD PE=3 SV=1
PSTB_BURSP 42 29824 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB OS=Burkholderia sp. GN=pstB PE=3 SV=1
PSTB_RALEJ 42 29899 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB OS=Ralstonia eutropha (strain JMP134) GN=pstB PE=3 SV=1
ASGR1_MOUSE 42 33153 1 (1) 1 (1) 0.07
Asialoglycoprotein receptor 1 OS=Mus musculus GN=Asgr1 PE=2 SV=3
CUX1_CANFA 42 105764 1 (1) 1 (1) 0.02
Homeobox protein cut-like 1 (Fragment) OS=Canis familiaris GN=CUX1 PE=2 SV=1
CUX1_HUMAN 42 164629 1 (1) 1 (1) 0.01
Homeobox protein cut-like 1 OS=Homo sapiens GN=CUX1 PE=1 SV=2
CUX1_MOUSE 42 165951 1 (1) 1 (1) 0.01
Homeobox protein cut-like 1 OS=Mus musculus GN=Cux1 PE=1 SV=3
PSTB2_ERWCT 42 29327 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB 2 OS=Erwinia carotovora subsp. atroseptica GN=pstB2 PE=3 SV=1
PSTB2_YERPA 42 29250 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=pstB2 PE=3 SV=1
PSTB2_YERPE 42 29250 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pestis GN=pstB2 PE=3 SV=1
PSTB2_YERPN 42 29250 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=pstB2 PE=3 SV=1
PSTB2_YERPS 42 29250 1 (1) 1 (1) 0.08
Phosphate import ATP-binding protein pstB 2 OS=Yersinia pseudotuberculosis GN=pstB2 PE=3 SV=1
RP54_RHOSH 42 48126 1 (1) 1 (1) 0.05
RNA polymerase sigma-54 factor OS=Rhodobacter sphaeroides GN=rpoN PE=3 SV=1
NANE_STAHJ 42 24809 1 (1) 1 (1) 0.09
Putative N-acetylmannosamine-6-phosphate 2-epimerase OS=Staphylococcus haemolyticus (strain JCSC1435) GN=nanE PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
308   332.1959 662.3772 662.3752 3.16 0 18 0.11 +1Score > 39 indicates identity
Score > 21 indicates homology		 R.FLDIR.R
485   374.2305 746.4464 746.4439 3.42 0 24 1.4 +4Score > 38 indicates identity E.IAQIFR.A
658   415.2449 828.4752 828.4705 5.74 1 42 0.046 +1Score > 42 indicates identity
Score > 41 indicates homology		 U K.EDIIAIR.G

+158

Accession Score Description
1 G3P_PSEAE 42 Glyceraldehyde-3-phosphate dehydrogenase OS=Pseudomonas aeruginosa GN=gap PE=3 SV=3

+159

Accession Score Description
1 RS13_CELJU 42 30S ribosomal protein S13 OS=Cellvibrio japonicus (strain Ueda107) GN=rpsM PE=3 SV=1

+160

Accession Score Description
1 APT_CAMJD 41 Adenine phosphoribosyltransferase OS=Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97) GN=apt PE=3 SV=1
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