MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 321 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 296 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 288 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 268 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 266 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 15.1 | SUCC_PSEPK |
266 | 41499 | 15 (11) | 11 (8) | 0.54 | |
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 | |||||||
| 3 samesets of SUCC_PSEPK | |||||||
| SUCC_PSEP1 |
266 | 41499 | 15 (11) | 11 (8) | 0.54 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sucC PE=3 SV=1 | |||||||
| SUCC_PSEPW |
266 | 41472 | 14 (11) | 10 (8) | 0.54 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain W619) GN=sucC PE=3 SV=1 | |||||||
| SUCC_PSEE4 |
266 | 41472 | 14 (11) | 10 (8) | 0.54 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas entomophila (strain L48) GN=sucC PE=3 SV=1 | |||||||
15 peptide matches (11 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 312 |
334.7277 | 667.4408 | 667.4381 | 4.10 | 0 | 25 | 0.12 | 1Score > 28 indicates identity |
K.VPVVVR.L | ||
| 486 |
374.2437 | 746.4728 | 746.4691 | 5.08 | 0 | 29 | 0.083 | 1Score > 32 indicates identity Score > 30 indicates homology |
K.IFVGLAK.L | ||
| 693 |
424.7363 | 847.4580 | 847.4552 | 3.36 | 0 | 43 | 0.035 | 1Score > 41 indicates identity |
U | K.QVQQFAK.I | |
| 1048 |
1 |
496.2734 | 990.5322 | 990.5287 | 3.60 | 0 | 39 | 0.0033 | 1Score > 41 indicates identity Score > 27 indicates homology |
U | K.AFAAQWLGK.N |
| 1076 |
501.2768 | 1000.5390 | 1000.5342 | 4.87 | 1 | 20 | 0.6 | 1Score > 41 indicates identity Score > 30 indicates homology |
U | K.IGGNEWVVK.A | |
| 1342 |
1 |
567.8187 | 1133.6228 | 1133.6081 | 13.0 | 1 | 31 | 0.041 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | K.ELYLGAVVDR.S |
| 1451 |
590.7960 | 1179.5774 | 1179.5706 | 5.80 | 0 | 58 | 0.00051 | 1Score > 41 indicates identity Score > 37 indicates homology |
U | K.INIDANAMYR.Q | |
| 1687 |
430.1918 | 1287.5536 | 1287.5480 | 4.32 | 0 | 23 | 0.056 | 1Score > 37 indicates identity Score > 23 indicates homology |
U | K.TFHDPSQDDAR.E | |
| 1821 |
1 |
676.3748 | 1350.7350 | 1350.7183 | 12.4 | 1 | 69 | 2.9e-006 | 1Score > 38 indicates identity Score > 26 indicates homology |
U | K.QLFAEYGLPVSK.G |
| 2274 |
1 |
785.4302 | 1568.8458 | 1568.8311 | 9.40 | 0 | 84 | 4.3e-007 | 1Score > 38 indicates identity Score > 33 indicates homology |
U | K.ATIDPLVGAQPFQGR.E |
| 2642 |
867.9395 | 1733.8644 | 1733.8584 | 3.47 | 0 | 81 | 2.6e-007 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | K.NLVTYQTDANGQPVSK.I |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| SUCC_PSEPG |
209 | 41413 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain GB-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSE14 |
188 | 41494 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sucC PE=3 SV=1 | |||||
| 4 samesets of SUCC_PSE14 | |||||
| SUCC_PSEFS |
188 | 41547 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEF5 |
188 | 41599 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSESM |
188 | 41472 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. tomato GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEU2 |
188 | 41442 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEA7 |
146 | 41772 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain PA7) GN=sucC PE=3 SV=1 | |||||
| 3 samesets of SUCC_PSEA7 | |||||
| SUCC_PSEAB |
146 | 41830 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEAE |
146 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa GN=sucC PE=3 SV=2 | |||||
| SUCC_PSEA8 |
146 | 41802 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain LESB58) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEPF |
125 | 41527 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf0-1) GN=sucC PE=3 SV=1 | |||||
| SUCC_PSEMY |
92 | 41759 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas mendocina (strain ymp) GN=sucC PE=3 SV=1 | |||||
| 1 sameset of SUCC_PSEMY | |||||
| SUCC_PSEU5 |
92 | 41761 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=sucC PE=3 SV=1 | |||||
| SUCC_MARAV |
89 | 41495 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_MARAV | |||||
| SUCC_SACD2 |
89 | 41524 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=sucC PE=3 SV=1 | |||||
| SUCC_TERTT |
89 | 41703 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBC3 |
81 | 41658 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sucC PE=3 SV=1 | |||||
| 2 samesets of SUCC_VIBC3 | |||||
| SUCC_VIBCH |
81 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae GN=sucC PE=3 SV=1 | |||||
| SUCC_VIBCM |
81 | 41658 | |||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain M66-2) GN=sucC PE=3 SV=1 | |||||
| SUCC_ACIAD |
31 | 41521 | 15.1 | ||
| Succinyl-CoA ligase [ADP-forming] subunit beta OS=Acinetobacter sp. (strain ADP1) GN=sucC PE=3 SV=1 | |||||
| 69 samesets of SUCC_ACIAD |
|||||
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 256 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 254 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 253 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 226 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 211 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
| 2 | PORF_PSESY | 177 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
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