MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 240)


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+141

Accession Score Description
1 RAVA_ECOBW 45 ATPase ravA OS=Escherichia coli (strain K12 / BW2952) GN=ravA PE=3 SV=1

+142

Accession Score Description
1 TILS_ACTPJ 44 tRNA(Ile)-lysidine synthase OS=Actinobacillus pleuropneumoniae serotype 3 (strain JL03) GN=tilS PE=3 SV=1

+143

Accession Score Description
1 SYFB_METLZ 44 Phenylalanyl-tRNA synthetase beta chain OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=pheT PE=3 SV=1

-144

Accession Score Description
1 HFQ_PSEPK 44 Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1
Score Mass Matches Sequences emPAI
144.1 HFQ_PSEPK 44 9456 3 (2) 3 (2) 0.58
Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1
1 sameset of HFQ_PSEPK
HFQ_PSEP1 44 9456 3 (2) 3 (2) 0.58
Protein hfq OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=hfq PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1928   468.2826 1401.8260 1401.8205 3.93 0 43 0.005 +1Score > 33 indicates identity
Score > 32 indicates homology
U K.HAISTVVPARPVR.L
2152   505.2641 1512.7705 1512.7685 1.32 0 21 0.5 +1Score > 40 indicates identity
Score > 30 indicates homology
K.GHSLQDPYLNTLR.K
2585   855.3690 1708.7234 1708.7176 3.40 1 31 0.026 +1Score > 36 indicates identity
Score > 28 indicates homology
U R.LPSPTDSEHGDSEPGNA.-

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
HFQ_PSEE4 43 9467 144.1
Protein hfq OS=Pseudomonas entomophila (strain L48) GN=hfq PE=3 SV=1
2 samesets of HFQ_PSEE4
HFQ_PSEPG 43 9426
Protein hfq OS=Pseudomonas putida (strain GB-1) GN=hfq PE=3 SV=1
HFQ_PSEPW 43 9442
Protein hfq OS=Pseudomonas putida (strain W619) GN=hfq PE=3 SV=1

+145

Accession Score Description
1 GCH1_PORG3 44 GTP cyclohydrolase 1 OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) GN=folE PE=3 SV=1

+146

Accession Score Description
1 EFP_PSEPG 43 Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1

+147

Accession Score Description
1 YLF8_SCHPO 43 UPF0662 protein C30C2.08 OS=Schizosaccharomyces pombe GN=SPAC30C2.08 PE=2 SV=1

+148

Accession Score Description
1 SUCC_COXBN 43 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+149

Accession Score Description
1 ETFA_PSEAE 43 Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1

+150

Accession Score Description
1 PYRD_SACKL 43 Dihydroorotate dehydrogenase, mitochondrial OS=Saccharomyces kluyveri GN=URA9 PE=1 SV=1
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